Aggregation induced emission (AIE) materials for mitochondria imaging.

Mitochondria are energy producing organelle of the eukaryotic cells. The main activities of mitochondria monitored by various marker molecules are autophagy detection, estimation of Reactive Oxygen Species (ROS), mitochondrial death and Photodynamic therapy in cancer cells. Due to the advantages of specificity and sensitivity, aggregation induced emission (AIE) is now popular for the mitochondria labeling. In this chapter, we would like to discuss three major types of AIEgens probe used in mitochondrial staining. There are three different types of AIEgens available for mitochondrial detection and sensing based on their different structural motifs. The first type of AIEgens is tetraphenylethene (TPE) based molecules. Due to simple engineering architecture, TPE based AIEgens are widely employed in bioimaging applications. AIEgen such as triphenylphosphine (TPP), and triphenylamine (TPA) are also employed as a novel building block. These are successfully used as exceptional lipid droplet (LD)-specific bio probes in cell imaging, assurance of cell combination, and photodynamic cancer cell removal. The third group is the miscellaneous AIEgens probe involved in mitochondria imaging.

Aggregation induced emission materials for tissue imaging.

Aggregation Induced Emission (AIE) has ample opportunities in the sensing and biomedical field. Recently, AIE molecular architecture, AIE nanoparticles (NPs) and AIE conjugated polymer (CP) probes have shown outstanding performance in bioimaging applications. In this chapter, we are summarizing the updated advancement in AIE based molecular, NP and CP probes for tissue imaging. We are focusing in vivo and in vitro tissue imaging with in-depth morphological and molecular information using microscopic techniques. Also, we have summarized various infrared windows for fluorescence microscopic technique to achieve deep penetration and high resolution of tissue images. In addition, future difficulties and challenges of AIEgens in tissue imaging are shortly discussed.

Patented AIE materials for biomedical applications.

In recent years aggregation induced emission (AIE) concept has attracted researcher's interest worldwide. Several organic building blocks are developed as AIE materials. This chapter discusses the patented AIE material and their utilization related in biological, medicinal and biotechnology fields. It is demonstrated that AIE chromophores such as tetraphenylethylene (TPE) as well as other AIE building blocks became important fluorescent emissive bioactive materials. Such emissive materials are widely employed as bioprobes for the detection of mitochondria, cellular imaging and tracking, protein carrier detection of S-phase DNA, detection of d-glucose, visualization of cancer treatment, drug screening, image-guided therapy, bacterial imaging, photodynamic therapy and drug screening. Such AIE materials upon imaging in cellular environment displays significant enhancement of fluorescence emission. Such patented AIE chromophores has a great potential for bioimaging and biomedical applications. In this chapter we compile some patented representative examples to explore their bioimaging/medicinal imaging applications since lot of new inventions are reported every day.