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Appl Environ Microbiol ; 76(8): 2673-7, 2010 Apr.
Article in English | MEDLINE | ID: mdl-20154108

ABSTRACT

The viral metagenome within an activated sludge microbial assemblage was sampled using culture-dependent and culture-independent methods and compared to the diversity of activated sludge bacterial taxa. A total of 70 unique cultured bacterial isolates, 24 cultured bacteriophages, 829 bacterial metagenomic clones of 16S rRNA genes, and 1,161 viral metagenomic clones were subjected to a phylogenetic analysis.


Subject(s)
Bacteria/classification , Bacteria/genetics , Biodiversity , Metagenome , Sewage/virology , Viruses/classification , Viruses/genetics , Bacteria/isolation & purification , Bacteriophages/classification , Bacteriophages/genetics , Bacteriophages/isolation & purification , Cluster Analysis , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Viral/chemistry , DNA, Viral/genetics , Molecular Sequence Data , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Virus Cultivation , Viruses/isolation & purification
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