ABSTRACT
alpha-l-Acetylmethadol is being currently evaluated as a substitute for methadone in the treatment of heroin addicts. The metabolites, isolated from urine, liver, and serum, were identified by gas-liquid chromatography. Methadone and its metabolites were found to be present in urine, liver and serum of rats treated with alpha-l-acetylmethadol. The presence of methadone was confirmed by gas-chromatography-mass spectometry. The purpose of this study was to determine whether alpha-l-acetylmethadol is metabolized to methadone, which might explain in part the longer duration of action of alpha-l-acetylmethadol.
Subject(s)
Methadone/analogs & derivatives , Methadyl Acetate/metabolism , Animals , Chromatography, Gas , Dealkylation , Liver/metabolism , Male , Methadone/metabolism , Methadyl Acetate/blood , Methadyl Acetate/urine , Oxidation-Reduction , RatsABSTRACT
Ketamine, (2-(o-chlorophenyl)-2-methylamine cyclohexanone) and its in vivo metabolite I (2-(o-chlorophenyl)-2-aminocyclohexanone) and metabolite II (2-(o-chlorophenyl)-2-amino-5-cyclohexene-1-one) were determined by thin-layer (TLC) and gas chromatography (GC). In vivo studies include intraperitoneal injection of ketamine in rats. Urine and blood samples were collected at regular intervals. The unreacted ketamine and its biotransformed products were extracted from urine and blood and subjected to TLC and GC analysis. 1% Carbowax-20M on Gas Chrom G-AW-DMCS and precoated LQ6D TLC plates were used in this study. In conclusion a rapid, simple, accurate, and sensitive thin-layer and gas chromatographic method for the identification of ketamine and its metabolites from biological fluids have been developed.
