ABSTRACT
Lymphoid enhancer-binding factor 1 (LEF1) has been regarded as an important gene for carcinogenesis in many malignancies, however, the role of LEF1 in the progression of human renal cell carcinoma (RCC) has not been well studied. In this study, we investigated the expression of LEF1 in human RCC and the effect on proliferative ability of RCC cells. RCC samples from 138 patients who underwent radical nephrectomy were used in this study, the expression of LEF1 protein was determined by immunohistochemistry and Western blot, mRNA expression was analyzed by RT-PCR and real-time PCR. To investigate the effect of LEF1 on the proliferation of RCC cells, a LEF1 vector was transfected into RCC cells and LEF1 expression was also decreased by using siRNA. Proliferative ability of RCC cells was examined by WST-1 assay and a xenograft study with BALB/C nude mice. Our results indicated that LEF1 expression was significantly increased in stage III, IV and grade 3 RCC than in normal kidney, however, decreased LEF1 expression was found in low-stage and grade RCC compared to that in normal kidney, the expression of LEF1 was correlated to tumor stages, histologic grade, and tumor sizes in RCC. The effect of LEF1 on the proliferation in RCC was also analyzed, our results suggested that RCC cells expressing high levels of LEF1 had significantly increased proliferative ability compared to control cell lines, in contrast, RCC cells with a low LEF1 expression had lower proliferative ability. Moreover, LEF1 promoted proliferation of RCC cells depending on suppressing G2/M cell-cycle arrest. Our study demonstrated that the expression of LEF1 is associated with the progression of RCC and that LEF1 maybe involved in the development of RCC, these suggested LEF1 play a key role and might serve as a therapeutic target in treating advanced RCC.
Subject(s)
Carcinoma, Renal Cell/pathology , Kidney Neoplasms/pathology , Lymphoid Enhancer-Binding Factor 1/metabolism , Adult , Aged , Aged, 80 and over , Animals , Carcinoma, Renal Cell/genetics , Cell Line, Tumor , Cell Proliferation , Female , Humans , Kidney/metabolism , Kidney/pathology , Kidney Neoplasms/genetics , Lymphoid Enhancer-Binding Factor 1/biosynthesis , Lymphoid Enhancer-Binding Factor 1/genetics , M Phase Cell Cycle Checkpoints/genetics , Male , Mice , Mice, Inbred BALB C , Mice, Nude , Middle Aged , Neoplasm Transplantation , RNA Interference , RNA, Messenger/biosynthesis , RNA, Small Interfering , Transplantation, HeterologousABSTRACT
OBJECTIVE: This study evaluated the long-term effects and clinical significance of latent abnormal pathology on elder living donor kidney graft function after renal transplantation in China. METHODS: One-hundred and thirty-eight living donor renal transplantations have been carried out at our hospital in recent years. Of these, 72 Time-Zero biopsies were performed and used in this analysis. Clinical data were retrospectively measured at 3, 6, 12, and 24 months after renal transplants. Relationships and effects from biopsy results taken from implanted donor kidney grafts were analyzed. RESULTS: Time-Zero biopsy pathology results from donor kidneys showed that 48.61% of donor kidneys had latent abnormal changes; arterial lesions of donor kidneys had significant effects on the renal function of grafts after 2 years' transplantation; correlations between donor age and arterial lesions were significant; and Time-Zero biopsy pathology results could help predict the long-term function of a renal graft. CONCLUSIONS: Existing latent pathological changes of an elder living donor kidney before transplantation could affect long-term renal function. Whether a senior donor is used should be very carefully considered.
Subject(s)
Allografts/pathology , Allografts/physiopathology , Kidney Transplantation/adverse effects , Adult , Age Factors , Biopsy , Female , Graft Rejection/drug therapy , Graft Rejection/prevention & control , Humans , Immunosuppressive Agents/therapeutic use , Kidney/pathology , Living Donors , Male , Middle Aged , Time Factors , Young AdultABSTRACT
BACKGROUND: To investigate the relationship between HLA-DR, DQ antigen, and intracranial aneurysm in the Han nationality. METHODS: We compared the occurrence of HLA-DR and HLA-DQ antigens in patients with intracranial saccular aneurysm and in the control group by PCR and agarose gel electrophoresis. RESULTS: No statistically significant correlation between HLA-DR, HLA-DQ antigen frequency, and the pathogenesis and clinical characteristics of intracranial aneurysm has been found. CONCLUSIONS: We have not found any relationship between HLA-DR and HLA-DQ antigen frequency, haplotype frequency, and pathogenesis and clinical characteristics of intracranial aneurysm, but the patients who presented with HLA-DR53, DR52, DQ7(3), and DQ5(1) seem to be more likely to bear multiple intracranial aneurysms.
