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1.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 32(6): 1207-11, 2015 Dec.
Article in Chinese | MEDLINE | ID: mdl-27079088

ABSTRACT

Pulse waves contain abundant physiological and pathological information of human body. Research of the relationship between pulse wave and human cardiovascular physiological parameters can not only help clinical diagnosis and treatment of cardiovascular diseases, but also contribute to develop many new medical instruments. Based on the traditional double elastic cavity model, the human cardiovascular system was established by using the electric network model in this paper. The change of wall pressure and blood flow in artery was simulated. And the influence of the peripheral resistance and vessel compliance to the distribution of blood flow in artery was analyzed. The simulation results were compared with the clinical monitoring results to predict the physiological and pathological state of human body. The result showed that the simulation waveform of arterial wall pressure and blood flow was stabile after the second cardiac cycle. With the increasing of peripheral resistance, the systolic blood pressure of artery increased, the diastolic blood pressure had no significant change, and the pulse pressure of artery increased gradually. With the decreasing of vessel compliance, the vasoactivity became worse and the pulse pressure increased correspondingly. The simulation results were consistent with the clinical monitoring results. The increasing of peripheral resistance and decreasing of vascular compliance indicated that the incidence of hypertension and atherosclerosis was increased.


Subject(s)
Heart/physiology , Models, Cardiovascular , Vascular Resistance , Aorta , Arteries/physiology , Atherosclerosis , Blood Pressure , Cardiovascular Diseases , Electricity , Hemodynamics , Humans , Hypertension
2.
Appl Biochem Biotechnol ; 166(5): 1148-66, 2012 Mar.
Article in English | MEDLINE | ID: mdl-22198867

ABSTRACT

A bacterial strain was isolated and cultured from the oil excavation areas in tropical zone in northern China. The biochemical characteristics and partial sequenced 16S rRNA gene of isolate, WJ-1, was identical to those of cultured representatives of the species Pseudomonas aeruginosa. This bacterium was able to produce a type of biosurfactant. Compositional analysis revealed that the extracted biosurfactant was composed of high percentage lipid (∼74%, w/w) and carbohydrate (∼20%, w/w) in addition to a minor fraction of protein (∼6%, w/w). The best production of 50.2 g/l was obtained when the cells were grown on minimal salt medium containing 6.0% (w/v) glucose and 0.75% (w/v) sodium nitrate supplemented with 0.1% (v/v) element solution at 37 °C and 180 rpm after 96 h. The optimum biosurfactant production pH value was found to be 6.0-8.0. The biosurfactant of WJ-1, with the critical micelle concentration of 0.014 g/L, could reduce surface tension to 24.5 mN/m and emulsified kerosene up to EI(24) ≈95. The results obtained from time course study indicated that the surface tension reduction and emulsification potential was increased in the same way to cell growth. However, maximum biosurfactant production occurred and established in the stationary growth phase (after 90 h). Thin layer chromatography, Fourier transform infrared spectrum, and mass spectrum analysis indicate the extracted biosurfactant was affiliated with rhamnolipid. The core holder flooding experiments demonstrated that the oil recovery efficiency of strain and its biosurfactant was 23.02% residual oil.


Subject(s)
Glycolipids/chemistry , Glycolipids/isolation & purification , Industrial Waste , Plant Oils/chemistry , Plant Oils/metabolism , Pseudomonas aeruginosa/metabolism , Surface-Active Agents/metabolism , Extracellular Space/metabolism , Glycolipids/metabolism , Hydrogen-Ion Concentration , Metals/chemistry , Micelles , Pseudomonas aeruginosa/cytology , Pseudomonas aeruginosa/isolation & purification , Salinity , Surface-Active Agents/chemistry , Surface-Active Agents/isolation & purification , Temperature
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