[Studies on the components of trace elements and macro elements in hemolymph of Anopheles anthropophagus].

The elements in hemolymph of An. anthropophagus were determined by ICAP. There were 18 kinds of trace elements such as Fe,Zn,Cu,Mn,Cr,Mo,Co,Ni,V,Sr,B,Al,Ba,Zr, Cd,Pb,Ga,Li and 6 kinds of macro elements such as Ca,Mg,K,Na,S,P in the hemolymph of the mosquito. The contents of the macro elements and Fe in hemolymph of newly emerged mosquitoes were significantly higher than those of mosquitoes after taking blood meal, whereas Zn and Al were lower. Comparing elements in hemolymph of An. anthropophagus and An. sinensis, there were 14 kinds of elements in newly emerged mosquitoes with striking significant difference, while there were 13 kinds of elements with striking significant difference in the mosquitoes after taking blood meal. Comparing elements in the hemolymph of An. anthropophagus and Ae. albopictus, there were 13 kinds of elements with striking significant difference in the hemolymph of newly emerged mosquitoes and the mosquitoes after taking blood meal. The results suggested that the components of elements in hemolymph were relevant to the nutritional metabolism and development of mosquitoes, and that mosquitoes of different species and with different sensibilities to malaria parasites also showed difference in the contents of elements in their hemolymph.

[Study on free amino acids and protein in hemolymph of Anopheles stephensi].

The changes in the contents of free amino acids in hemolymph of Anopheles stephensi were determined by automatic amino acid analyzer. The changes in hemolymph protein were determined by ultraviolet absorption method. Free amino acids in hemolymph of infected mosquitoes were compared with those in noninfected mosquitoes. At 4 days after blood meal, 6 kinds of amino acids decreased markedly, and 5 kinds of amino acids increased markedly; at 7 days after blood meal, 4 kinds of amino decreased markedly, while 7 kinds of amino acids increased markedly; at 11 days after acids blood meal, 9 kinds of amino acids decreased markedly, and 4 kinds of amino acids increased remarkably. The protein concentration of infected mosquitoes was higher than that of noninfected ones.

[Diagnosis of neurocysticercosis by immunoenzymatic staining technique (IEST) with frozen sections of Cysticercus cellulosae].

This article deals with the sensitivity and specificity of IEST in neurocysticercosis with frozen sections of Cysticerus cellulosae as antigens. 55 out of 56 cases (98.2%) of neurocysticercosis showed positive reaction. All serum specimens from healthy human controls (50 cases), clonorchiasis (23 cases) and paragonimiasis (26 cases) were negative, but 18% (4/22) of serum specimens from echinococcosis revealed positive reactions. The results indicate that IEST is specific, sensitive, economical and easy to perform for the diagnosis of neurocysticercosis.