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1.
Neurourol Urodyn ; 36(4): 1061-1068, 2017 04.
Article in English | MEDLINE | ID: mdl-27490041

ABSTRACT

BACKGROUND & AIM: Alpha lipoic acid (LA) was shown to exert neuroprotection in trauma-induced spinal cord injury (SCI), which is frequently associated with urinary bladder complaints in patients with SCI. Accordingly, the protective effects of LA on biochemical and histological changes in bladder as well as functional studies were assessed. METHODS: Wistar albino rats were divided as control, SCI, and LA (50 mg/kg/day, ip) treated SCI groups (SCI+LA). The standard weight-drop (100 g/cm force at T10) method was used to induce a moderately severe SCI. One week after the injury, neurological examination was performed and the rats were decapitated. Bladder samples were taken for histological examination, functional (isolated tissue bath) studies, and for the measurement of biochemical parameters (malondialdehyde, MDA; gluthathione, GSH; nerve growth factor, NGF; caspase-3, luminol and lucigenin chemiluminescences). RESULTS: SCI caused a significant (P < 0.001) increase in the detrusor muscle thickness. It increased the contractility responses to carbachol and relaxation responses to papaverine (P < 0.05-0.001). There were also significant alterations in MDA, caspase-3, luminol, and lucigenin chemiluminescences with concomitant decreases in NGF and GSH (P < 0.05). LA treatment reversed histological and functional (contraction and relaxation responses) changes induced by SCI (P < 0.05-0.001), but no significant recovery was observed in the impaired neurological functions. CONCLUSION: These results indicate that LA have a beneficial effect in improving the bladder tonus via its antioxidant and anti-inflammatory actions following SCI.


Subject(s)
Antioxidants/administration & dosage , Spinal Cord Injuries/complications , Thioctic Acid/administration & dosage , Urinary Bladder Diseases/drug therapy , Urinary Bladder/drug effects , Animals , Disease Models, Animal , Male , Rats , Rats, Wistar , Urinary Bladder/innervation , Urinary Bladder/pathology , Urinary Bladder/physiopathology , Urinary Bladder Diseases/etiology
2.
Int J Neurosci ; 121(3): 142-8, 2011 Mar.
Article in English | MEDLINE | ID: mdl-21138398

ABSTRACT

Traumatic injury to the central nervous system results in the delayed dysfunction and neuronal death. Impaired mitochondrial function, generation of reactive oxygen species (ROS), and lipid peroxidation occur soon after traumatic spinal cord injury (SCI), while the activation of compensatory molecules that neutralize ROS occurs at later time points. The aim of the current study was to investigate the putative neuroprotective effect of the COX2 inhibitor meloxicam in a rat model of SCI. In order to induce SCI, a standard weight-drop method that induced a moderately severe injury (100 g/cm force) at T10, was used. Injured animals were given either 2 mg/kg meloxicam or saline 30 min postinjury by intraperitoneal injection. At seven days postinjury, neurological examination was performed and rats were decapitated. Spinal cord samples were taken for histological examination or determination of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and DNA fragmentation. Formation of ROS in spinal cord tissue samples was monitored by using a chemiluminescence (CL) technique. SCI caused a significant decrease in spinal cord GSH content, which was accompanied with significant increases in CL, MDA levels, MPO activity, and DNA damage. On the other hand, meloxicam treatment reversed all these biochemical parameters as well as SCI-induced histopathological alterations. Furthermore, impairment of the neurological functions due to SCI was improved by meloxicam treatment. The present study suggests that meloxicam, reduces SCI-induced oxidative stress and exerts neuroprotection by inhibiting lipid peroxidation, GSH depletion, and DNA fragmentation.


Subject(s)
Cyclooxygenase 2 Inhibitors/therapeutic use , Neuroprotective Agents/therapeutic use , Spinal Cord Injuries/drug therapy , Thiazines/therapeutic use , Thiazoles/therapeutic use , Animals , DNA Fragmentation/drug effects , Glutathione/analysis , Lipid Peroxidation/drug effects , Luminescent Measurements , Malondialdehyde/analysis , Meloxicam , Motor Activity , Peroxidase/analysis , Rats , Rats, Wistar , Reactive Oxygen Species/analysis , Spinal Cord/chemistry , Spinal Cord/pathology , Spinal Cord Injuries/metabolism , Spinal Cord Injuries/pathology
3.
J Spinal Cord Med ; 33(4): 401-9, 2010.
Article in English | MEDLINE | ID: mdl-21061900

ABSTRACT

BACKGROUND: Oxidative stress is a mediator of secondary injury to the spinal cord following trauma. OBJECTIVE: To investigate the putative neuroprotective effect of alpha-lipoic acid (LA), a powerful antioxidant, in a rat model of spinal cord injury (SCI). METHODS: Wistar albino rats were divided as control, vehicle-treated SCI, and LA-treated SCI groups. To induce SCI, a standard weight-drop method that induced a moderately severe injury (100 g/cm force) at T10 was used. Injured animals were given either 50 mg/kg LA or saline at 30 minutes postinjury by intraperitoneal injection. At 7 days postinjury, neurologic examination was performed, and rats were decapitated. Spinal cord samples were taken for histologic examination or determination of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity, and DNA fragmentation. Formation of reactive oxygen species in spinal cord tissue samples was monitored by using a chemiluminescence (CL) technique. RESULTS: SCI caused a significant decrease in spinal cord GSH content, which was accompanied with significant increases in luminol CL and MDA levels, MPO activity, and DNA damage. Furthermore, LA treatment reversed all these biochemical parameters as well as SCI-induced histopathologic alterations. Conversely, impairment of the neurologic function caused by SCI remained unchanged. CONCLUSION: The present study suggests that LA reduces SCI-induced oxidative stress and exerts neuroprotection by inhibiting lipid peroxidation, glutathione depletion, and DNA fragmentation.


Subject(s)
DNA Fragmentation/drug effects , Neuroprotective Agents/therapeutic use , Spinal Cord Injuries/drug therapy , Thioctic Acid/therapeutic use , Analysis of Variance , Animals , Disease Models, Animal , Glutathione/metabolism , Luminescent Measurements/methods , Male , Malondialdehyde/metabolism , Neurologic Examination , Neuroprotective Agents/pharmacology , Oxidative Stress/drug effects , Peroxidase/metabolism , Rats , Rats, Wistar , Reactive Oxygen Species/metabolism , Spinal Cord Injuries/metabolism , Spinal Cord Injuries/pathology , Spinal Cord Injuries/physiopathology , Thioctic Acid/pharmacology
4.
Neurol Res ; 32(6): 629-35, 2010 Jul.
Article in English | MEDLINE | ID: mdl-19660237

ABSTRACT

OBJECTIVE: The overproduction of reactive oxygen species and resultant damage to cellular proteins or lipids of cell membranes and DNA by free radicals are the underlying mechanisms of many neuropathologies. Cyclooxygenase-2 (COX-2) inhibitors have been suggested to be neuroprotective by reducing prostanoid and free radical synthesis, or by directing arachidonic acid metabolism through alternate pathways. This study investigated the putative neuroprotective effect of the COX-2 inhibitor, meloxicam, in a rat model of diffuse brain injury. METHODS: Sprague-Dawley rats were subjected to traumatic brain injury with a weight-drop device using 300 g(-1) m weight-height impact. The groups were: control, meloxicam (2 mg/kg, i.p.), trauma and trauma + meloxicam (2 mg/kg, i.p.). Forty-eight hours after the injury, neurological examination scores were measured, the animals were decapitated and brain tissues were taken. Brain edema and blood-brain barrier (BBB) permeability were evaluated by wet-dry weight method and Evans blue (EB) extravasation respectively. In brain tissues, malonedialdehyde, glutathione, myeloperoxidase and Na/K-ATPase levels were measured. RESULTS: The neurological examination scores mildly increased in trauma groups 48 hours after the induction of trauma. Meloxicam treatment improved the altered neurological status. The trauma caused a significant increase in brain water content that was partially reversed by meloxicam. Meloxicam also reduced the EB extravasation indicating the preservation of the BBB integrity. Meloxicam treatment also significantly reduced the increase in malondialdehyde and myeloperoxidase levels and restored glutathione content of the brains that had been significantly increased after trauma. CONCLUSION: Meloxicam exerts neuroprotective effect by preserving BBB permeability and by reducing brain edema (probably by its anti-inflammatory properties) in the diffuse brain injury model.


Subject(s)
Blood-Brain Barrier/drug effects , Brain Chemistry/drug effects , Brain Injuries , Cyclooxygenase 2 Inhibitors/therapeutic use , Thiazines/therapeutic use , Thiazoles/therapeutic use , Analysis of Variance , Animals , Brain Injuries/drug therapy , Brain Injuries/pathology , Brain Injuries/physiopathology , Cyclooxygenase 2 Inhibitors/pharmacology , Disease Models, Animal , Glutathione/metabolism , Male , Malondialdehyde/metabolism , Meloxicam , Neurochemistry/methods , Neurologic Examination/methods , Permeability/drug effects , Peroxidase/metabolism , Rats , Rats, Sprague-Dawley , Sodium-Potassium-Exchanging ATPase/metabolism
5.
Turk Neurosurg ; 19(4): 400-5, 2009 Oct.
Article in English | MEDLINE | ID: mdl-19847762

ABSTRACT

PURPOSE: Spinal dysraphisms are the most common congenital anomalies of the CNS. Spinal dysraphism (SD) of cervical and upper thoracic region are rare, demonstrating distinct clinical and structural configurations compared to lumbar counterparts. METHODS: In Haydarpasa Numune Hospital, a total of 7 cases (3 male and 4 female) ranging between 20 days to 9 years of age with cervicothoracic SD were operated on between 2002 and 2008. The sacs were located in the cervical and thoracic region in 4 and 3 of the cases, respectively. The associated anomalies were diagnosed in 3 cases, including SCM type 2, Chiari type 2, hyrocephalus and hydromyelia. All cases underwent surgical treatment that involves excision of the sac and intradural exploration providing untethering of the spinal cord. Postoperative follow up of these patients was uneventful and neither neurological deficits nor complications were observed. CONCLUSION: Cervicothoracic SD has more favorable outcome in respect to neurological, orthopaedic and urologic problems compared to lumbar counterparts. In order to prevent forthcoming neurological deterioration, surgical treatment consisting of intradural exploration of the lesion, untethering of the spinal cord and excision of potential adhesions should be performed in the early period.


Subject(s)
Cervical Vertebrae , Magnetic Resonance Imaging , Spinal Dysraphism/pathology , Spinal Dysraphism/surgery , Thoracic Vertebrae , Child , Child, Preschool , Female , Humans , Hydrocephalus/diagnostic imaging , Infant , Infant, Newborn , Male , Spinal Dysraphism/diagnostic imaging , Tomography, X-Ray Computed , Treatment Outcome
6.
Brain Inj ; 23(6): 577-84, 2009 Jun.
Article in English | MEDLINE | ID: mdl-19484631

ABSTRACT

INTRODUCTION: Traumatic brain injury is highly associated with the over-production of reactive oxygen species. The aim of this study was to investigate the putative neuroprotective effect of montelukast, a cysteinyl-leukotriene receptor antagonist, in a rat model of traumatic brain injury (TBI). METHODS: Sprague Dawley rats were subjected to TBI with a weight-drop device using 300 g-1 m weight-height impact. The groups were: control (saline), montelukast (10 mg kg(-1) per day, ip), trauma and trauma + montelukast. Two days post-trauma, neurological examination scores were measured and animals were decapitated and the brain tissues were taken for the histologic and biochemical [malondialdehyde (MDA)-an index for lipid peroxidation, reduced glutathione (GSH), myeloperoxidase (MPO)-an index for neutrophil infiltration and Na+/K+-ATPase activity] evaluations. Brain oedema and blood-brain barrier (BBB) permeability were also evaluated. RESULTS: The neurological examination scores mildly increased in trauma groups at 48 hours. Although the scores were decreased in the montelukast treated group, they were still significantly higher than the control. The trauma caused a significant increase in brain water content and Evans blue (EB) extravasation. Montelukast treatment reduced BBB permeability. It also decreased lipid peroxidation and MPO activity. CONCLUSION: The present study suggests that montelukast may have beneficial effects against TBI-induced oxidative stress of the brain.


Subject(s)
Acetates/pharmacology , Blood-Brain Barrier/drug effects , Brain Edema/prevention & control , Brain Injuries/complications , Immunologic Factors/pharmacology , Leukotriene Antagonists/pharmacology , Quinolines/pharmacology , Animals , Brain Edema/etiology , Cyclopropanes , Neuroprotective Agents/pharmacology , Oxidative Stress/drug effects , Permeability , Rats , Rats, Sprague-Dawley , Sulfides
7.
Free Radic Res ; 43(7): 658-67, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19468925

ABSTRACT

Traumatic brain injury (TBI) was induced by a weight-drop device using 300 g-1 m weight-height impact. The study groups were: control, alpha-lipoic acid (LA) (100 mg/kg, po), TBI, and TBI + LA (100 mg/kg, po). Forty-eight hours after the injury, neurological scores were measured and brain samples were taken for histological examination or determination of thiobarbituric acid reactive substances (TBARS) and glutathione (GSH) levels, myeloperoxidase (MPO) and Na(+)-K(+) ATPase activities, whereas cytokines (TNF-alpha, IL-1beta) were determined in blood. Brain oedema was evaluated by wet-dry weight method and blood-brain barrier (BBB) permeability was evaluated by Evans Blue (EB) extravasation. As a result, neurological scores mildly increased in trauma groups. Moreover, TBI caused a significant decrease in brain GSH and Na(+)-K(+) ATPase activity, which was accompanied with significant increases in TBARS level, MPO activity and plasma proinflammatory cytokines. LA treatment reversed all these biochemical indices as well as histopathological alterations. TBI also caused a significant increase in brain water content and EB extravasation which were partially reversed by LA treatment. These findings suggest that LA exerts neuroprotection by preserving BBB permeability and by reducing brain oedema probably by its anti-inflammatory and antioxidant properties in the TBI model.


Subject(s)
Antioxidants/pharmacology , Brain Edema/prevention & control , Brain Injuries/prevention & control , Neuroprotective Agents/pharmacology , Thioctic Acid/pharmacology , Animals , Blood-Brain Barrier , Brain Edema/metabolism , Brain Edema/pathology , Brain Injuries/metabolism , Brain Injuries/pathology , Glutathione/metabolism , Interleukin-1beta/metabolism , Oxidative Stress/drug effects , Peroxidase/metabolism , Rats , Rats, Wistar , Thiobarbituric Acid Reactive Substances/metabolism , Tumor Necrosis Factor-alpha/metabolism
8.
Turk Neurosurg ; 17(4): 273-6, 2007 Oct.
Article in English | MEDLINE | ID: mdl-18050072

ABSTRACT

We present a rare case of agenesis of the left internal carotid artery in a 43-year old woman, associated with an aneurysm of the anterior communicating artery and presenting with subarachnoid hemorrhage. The left internal carotid artery was not visualized on the left carotid angiogram. The left middle cerebral artery was perfused from the basilar artery via the dilated posterior communicating artery on vertebral angiogram. Absence of the left carotid canal was proven on temporal bone computed tomography. Absence of the left internal carotid artery was verified at operation. Absence of internal carotid artery is discussed in relation to aneurysm formation.


Subject(s)
Anterior Cerebral Artery/abnormalities , Arteriovenous Fistula/etiology , Carotid Artery, Internal/abnormalities , Adult , Anterior Cerebral Artery/diagnostic imaging , Anterior Cerebral Artery/physiopathology , Arteriovenous Fistula/diagnostic imaging , Arteriovenous Fistula/physiopathology , Carotid Artery, Internal/diagnostic imaging , Carotid Artery, Internal/physiopathology , Cerebrovascular Circulation/physiology , Female , Humans , Hydrocephalus/etiology , Hydrocephalus/pathology , Subarachnoid Hemorrhage/diagnostic imaging , Subarachnoid Hemorrhage/etiology , Subarachnoid Hemorrhage/physiopathology , Tomography, X-Ray Computed
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