ABSTRACT
OBJECTIVES: To evaluate the effect of demographic and occupational factors on the severity of the acute occupational hand injuries. METHODS: Patients with acute hand injuries presenting to the emergency department of the Ege university hospital between 01.08.2008 and 27.02.2009 were included. A questionnaire investigating demographic and occupational factors of the patients and their injuries was filled out for each patient. Modified Hand Injury Severity Score (MHISS) was used to assess the severity of the injury. RESULTS: A total of 144 subjects were included. Forty-three patients had occupational hand injuries. Age at injury, occupation, and main earning status did not alter the MHISS score significantly. Also, the mechanism of injury, occupational experience, timing of the injury, glove use, safety training did not have a significant effect on the injury severity. The injury pattern was found to have a statistically significant effect on the MHISS score. CONCLUSION: The potentially modifiable factors such as the working conditions, safety training and use of gloves are important in the etiology of occupational acute hand injury. Most probably, in this study the size of the sample was not large enough to be able to demonstrate any relation between these and the injury severity. However, attempts to modify these factors by various strategies may reduce the incidence of acute hand injury at work. Precautions and widespread educational programs can prevent occupational acute hand injury.
Subject(s)
Hand Injuries/etiology , Occupational Injuries/etiology , Adolescent , Adult , Aged , Child , Child, Preschool , Female , Gloves, Protective , Humans , Infant , Injury Severity Score , Male , Middle Aged , Risk Assessment , Risk Factors , Safety Management , Young AdultABSTRACT
The interaction of ammonium trioxovanadate(V) with cysteine in aqueous solution was studied by cyclic voltammetry and absorption spectroscopy techniques. In the absence of cysteine, the cyclic voltammogram (CV) of ammonium trioxovanadate(V) solution in 0.1 M phosphate buffer (pH 7) gave two peaks at -0.130 V (reversible) and -0.400 V (irreversible). These peaks (-0.130 V, -0.400 V) can be attributed to V(V)/V(IV) and V(IV)/V(III) redox processes, respectively. In the presence of cysteine at low scan rate (40 mV/s), the peak at -0.780 V, which is assigned to the irreversible reduction of free cystine, was observed. In addition, the reduction peak of the disulfidic anion S(2)(2-) was seen at -0.650 V. Under aerobic conditions, the peaks of the disulfidic anion S(2)(2-) and free cystine are well separated. From electronic spectra of ammonium trioxovanadate(V) and cysteine mixtures, LMCT transition associated with V(V)-cyteine complex was obtained at 743 nm. The stoichiometry (ML(2)) and stability constant (log beta(1:2)=6.67) of V(V)-cysteine complex were determined by means of mole ratio method.
Subject(s)
Cysteine/chemistry , Organometallic Compounds/chemistry , Electrodes , Organometallic Compounds/metabolism , Oxidation-Reduction , Oxygen/metabolism , Potentiometry , Quaternary Ammonium Compounds/chemistry , Spectrum Analysis , Sulfhydryl Compounds/chemistry , Sulfhydryl Compounds/metabolism , Vanadium/chemistryABSTRACT
The interaction of doxorubicin with Fe(III) ions and nicotinamide (NA) has been followed by square-wave voltammetry, cyclic voltammetry and UV-VIS. spectroscopy techniques at aerobic and anaerobic conditions. Fe(III)-doxorubicin complex gives a 1-electron reversible step at -0.494 V and a shoulder at 580 nm. Further, the Fe(III)-doxorubicin complex was found to be more stable at aerobic conditions. In the presence of NA, an intermediate (NA-Fe(III)-DQ) forms at -0.462 V under aerobic conditions. Because of the formation of this intermediate, nicotinamide may reduce the cardiotoxic effect of doxorubicin and cause to its detoxification.
Subject(s)
Doxorubicin/chemistry , Iron/chemistry , Niacinamide/chemistry , Oxygen/chemistry , Electrochemistry , Ions/chemistry , Molecular Structure , Spectrophotometry , Time FactorsABSTRACT
Circulating leptin levels are elevated during the later stages of pregnancy in mammals, suggesting that maternal leptin may play a role in maintenance of pregnancy and/or preparation for parturition and lactation. The regulation and source of circulating leptin during pregnancy remains undetermined, but leptin mRNA levels increase in adipose tissue during this time in some species. Considerable controversy exists whether placenta is also a leptin-secreting tissue during pregnancy. Here, we directly demonstrate that leptin secretion rates from mouse adipose tissue in vitro are decreased during early pregnancy and up-regulated during late pregnancy and lactation. Changes in leptin secretion rates in vitro paralleled those of circulating leptin in vivo during gestation. Subcutaneous implants of estradiol or corticosterone into lactating mice for 48 h stimulated adipose leptin secretion rates in vitro to the level of that in pregnant mice. However, corticosterone, but not estradiol, increased leptin secretion when added to isolated adipose tissue in vitro. Placentae obtained at two stages of pregnancy did not secrete leptin in vitro, either when acutely isolated or when dissociated into cells for long-term cultures. Placental tissue (or cells) secreted progesterone, however, demonstrating placental viability. We conclude that hyperleptinemia during late pregnancy in mice primarily results from corticosterone-dependent up-regulation of leptin secretion from adipose tissue, and that the placenta does not contribute to leptin secretion. The initial decrease in leptin secretory rates from adipose tissue during early pregnancy may facilitate energy storage for the subsequent, increased metabolic demands of later pregnancy and lactation.
Subject(s)
Adipose Tissue/metabolism , Leptin/metabolism , Pregnancy, Animal/metabolism , Steroids/metabolism , Adipose Tissue/drug effects , Animals , Corticosterone/metabolism , Corticosterone/pharmacology , Estradiol/metabolism , Estradiol/pharmacology , Female , In Vitro Techniques , Lactation , Mice , Placenta/metabolism , Pregnancy , Progesterone/metabolism , Steroids/pharmacology , Up-RegulationABSTRACT
We previously demonstrated high leptin levels during late pregnancy in little brown bats (Myotis lucifugus). We now extend these observations to a second species, the big brown bat (Eptesicus fuscus), and also report that leptin increases after the first trimester of pregnancy. Leptin decreased to baseline 1 week following parturition, with a half-time decay of 2 days. During lactation, leptin was significantly correlated with body mass in E. fuscus, but not in M. lucifugus. No circadian pattern of leptin was observed in M. lucifugus. The decrease in post-partum leptin in bats may be partly explained by loss of putative placental leptin. The continued decrease may reflect depletion of body fat during this energy demanding period, at least in Eptesicus. Changes in leptin during lactation appeared to be independent of circadian effects and time of sampling. Our study provides additional evidence that leptin increases during pregnancy and declines during lactation in a free-ranging mammal, supporting the hypothesis that leptin plays important but yet undetermined roles in reproduction.
Subject(s)
Chiroptera/blood , Lactation/blood , Proteins/metabolism , Animals , Circadian Rhythm , Female , Leptin , Pregnancy , Species SpecificityABSTRACT
The square-wave adsorptive stripping voltammetric behaviour of cysteine at the physiological pH (7.40) has been optimised with respect to accumulation time, accumulation potential, scan rate and drop size. This study describes the application of voltammetry to studies on the interaction of cysteine with monosaccharides.
