ABSTRACT
PURPOSE: To report a series of patients with ocular complications associated with laser-assisted eyebrow hair removal. PATIENTS AND METHODS: Case reports of three patients with eye pain and photophobia following laser epilation of the eyebrow region. The eye examination included visual acuity, slit-lamp examination, tonometry and fundoscopy. The follow-up period was 3 months. RESULTS: Each patient had conjunctival hyperaemia in one or both eyes and anterior chamber pigmentary cells. One patient presented with posterior synechiae, which did not respond to treatment. CONCLUSIONS: Laser epilation of the eyebrows may result in anterior uveitis as well as irreversible damage to the iris.
Subject(s)
Eye Diseases/etiology , Eyebrows , Hair Removal/adverse effects , Laser Therapy/adverse effects , Administration, Topical , Adult , Eye Diseases/drug therapy , Female , Hair Removal/methods , Humans , Mydriatics/administration & dosage , Ophthalmic Solutions/administration & dosage , Pupil Disorders/drug therapy , Pupil Disorders/etiology , Steroids/therapeutic use , Treatment Outcome , Uveitis/drug therapy , Uveitis/etiology , Visual Acuity , Young AdultABSTRACT
Transforming Ha-ras enhances the mitogen-induced activation of both the Ca(2+)-influx and the furosemide-sensitive Na+/K+/2Cl-cotransporter. Both systems represent essential early steps of mitogenic signal transduction in NIH 3T3 fibroblasts.
Subject(s)
Calcium/metabolism , Mitogens/pharmacology , Oncogene Protein p21(ras)/pharmacology , Potassium/metabolism , 3T3 Cells , Animals , Biological Transport, Active/drug effects , Bombesin/pharmacology , Bradykinin/pharmacology , Cell Division/drug effects , Fibroblasts , Furosemide/pharmacology , Mice , Rubidium/metabolism , TransfectionABSTRACT
In NIH-3T3 fibroblasts expressing the ras oncogene (+ras) bradykinin (BK) elicits sustained oscillations (1/min) of cell membrane potential (PD) due to oscillations of intracellular calcium activity with subsequent activation of calcium sensitive K+ channels. In NIH-3T3 fibroblasts not expressing the oncogene (-ras), BK leads to a single transient hyperpolarization of the cell membrane, not followed by oscillations. The oscillations of cell membrane potential require the presence of extracellular calcium and are abolished by K+ channel blocker barium (1 mmol/l), as well as by calcium channel blockers cadmium (1 mmol/l), lanthanum (0.1 mmol/l) and nifedipine (10 mumol/l). However, the oscillations are not modified by 1 mumol/l nifedipine, or by other calcium channel blockers, such as verapamil (10 mumol/l) or diltiazem (10 mumol/l). Cell proliferation is inhibited by nifedipine (10 mumol/l) but not by verapamil or diltiazem, indicating that the oscillations of intracellular calcium are a prerequisite for the growth factor independent proliferation of ras oncogene expressing cells.
