Internal dynamics of human ubiquitin revealed by 13C-relaxation studies of randomly fractionally labeled protein.

The use of random, fractional 13C-enrichment combined with low pass filtration has allowed the determination of NMR relaxation parameters at an unprecedented number of sites within recombinant human ubiquitin. Essentially complete 1H, 13C, and 15N resonance assignments for the protein are reported. Carbon spin lattice and heteronuclear NOE relaxation data have been analyzed in the context of the Lipari-Szabo "model free" formalism. The generalized order parameters for 56 main chain alpha C-H vectors have been determined and are found to correspond to the highly restricted motion seen in previous studies of the motion of amide N-H vectors. In distinct contrast, the analysis presented here indicates an unexpected range of dynamics within the interior of the protein. The generalized order parameters of 45 methyl groups of human ubiquitin have been determined. The methyl groups of Thr and Ala residues show generalized order parameters ranging from the Woessner limit (0.111) to below 0.01. Generalized order parameters for all methyl groups of the seven isoleucine residues were determined. With one exception, the generalized order parameters of the gamma methyls were equal to or greater than the corresponding delta methyls, indicating higher mobility away from the main chain. Generalized order parameters for 11 methyl groups of leucine residues were also determined. In six of the seven cases where the generalized order parameters of both prochiral methyl groups were determined, the pro-R methyl consistently shows a higher value than the pro-S methyl group. Generalized order parameters for seven methyl groups of four valines were also determined. There is no apparent correlation of methyl group prochirality with the value of the generalized order parameter. These data have several implications and generally indicate that the interior of the protein is heterogeneously dynamic.

[Mortality differences in lung surgery]. / Letalitätsunterschiede bei Lungenoperationen.

The surgical mortality rate in a total of 1,100 patients with lung cancer was investigated. Since it was found that, in part, mortality extended to beyond 30 days, the differences to be seen on the 50th post-operative day are presented. A considerable negative bias was established for upper, as compared with lower, lobe resection, a negative bias for resection on the right side, and a special negative bias for right-sided pulmonectomy - which, till now, had also been considered to exist for bilobectomy of the lower and middle lobes. It is suggested that these differences be taken into account when assessing operability and preparing surgical statistics.

[Preoperative lung function data of patients dying within 50 days and of surgical patients surviving longer than 6 months]. / Präoperative Lungenfunktionsdaten von innerhalb 50 Tagen Verstorbenen und von Resezierten, die länger als sechs Monate überlebten.

A sequence of parameters is presented in the order of importance for functional operational ability diagnosis obtained from quotients of preoperative averages of findings in patients who dies postoperatively and in survivors. It appears that the following parameters are particularly important: pressure of a. pulmonalis under stress, perfusion scintigram and respiratory gas analyses, whereas e.g. in TGV%N, BGA under stress and FEV1 predicted via scintigram, no differences worth mentioning were seen. Reference is made to differences in respiratory and blood gas analysis before and after resection of the upper and lower lobes.

[Crux medicorum: Ondine's curse (author's transl)]. / Crux medicorum: Undine's Fluch.

All known cases of the Ondine's curse syndrome with lifethreatening complications in consequence of narcosis, sedatives or oxygen-administration are reported. The avoidance of accidents requires blood gas analysis and more attention to the less well-known phenomenon--possibly a cause of the sudden-death-syndrome.

[On respiratory stimulation by aminophenazol in global insufficiency due to chronic-obstructive diseases of the lungs (author's transl)]. / Zur Atemstimulation mit Amiphenazol bei Globalinsuffizienz infolge chronisch obstruktiver Lungenkrankheit

For evaluation of a respiratory analeptic as supplementary therapy in global insufficiency blood gas analyses were performed on 19 patients under normal room air conditions as well as under oxygen breathing. It could be proved that with 2,4-diamino-5-phenylthiazol (aminophenazol; Daptazile) respiratory depression associated associated with chronic obstructive pulmonary diseases can be influenced and that here is statistically significant improvement of respiration. Effects of mechanical breathing and hyperventilatin are discussed -- basing on the present evaluation results they need not be expected. Side effects were not registered. With simultaneous and exhaustive application of all therapeutic means for the remedy of pulmonary obstruction amiphenazol is recommended for use in cases of pulmonary hypoventilation and primary central respiratory depression.