Spectrofluorimetric Analysis of Riboflavin Content during Kombucha Fermentation.

Kombucha is a traditional beverage obtained by the microbial fermentation of tea using a symbiotic culture of bacteria and yeasts. In addition to several documented functional properties, such as anti-inflammatory activity and antioxidant activity, kombucha is often credited with high levels of vitamins, including riboflavin. To our knowledge, the vitamin B2 content in traditionally prepared kombucha has been determined in only two studies, in which the concentration measured by the HPLC technique ranged from 2.2 × 10-7 to 2.1 × 10-4 mol dm-3. These unexplained differences of three orders of magnitude in the vitamin B2 content prompted us to determine its concentration during the cultivation of kombucha under very similar conditions by spectrofluorimetry. The B2 concentrations during the 10-day fermentation of black tea ranged from 7.6 × 10-8 to 3.3 × 10-7 mol dm-3.

Synthesis of Hydroxyapatite/Iron Oxide Composite and Comparison of Selected Structural, Surface, and Electrochemical Properties.

The paper presents the synthesis of a hydroxyapatite/iron oxide composite utilizing the wet chemical method, as well as the comparison of several selected material characteristics. As follows from the literature reports, hydroxyapatite is a common mineral possessing numerous significant properties. Nowadays, there is an increase in the amount of research on possible modifications of this compound. The promising way to improve hydroxyapatite features is its combination with iron oxide. Particularly, there can be two forms that are distinguished, namely Fe3O4 and γ-Fe2O3. These oxides exhibit valuable properties, particularly magnetism. A combination of the mentioned materials leads to multifunctional composite formation with many potential applications, as follows from several studies. However, this area of science is not fully developed. There are still many aspects to be examined. The synthesized composite and its components were analyzed by employing the following methods. The X-ray diffraction analysis revealed formation of hydroxyapatite and Fe2O3 crystalline phases. Moreover, porosimetry proved a larger specific area for the composite sample in comparison with other materials. The results obtained using the SEM method confirmed an external layer of hydroxyapatite and spherical shapes of internal Fe2O3 particles. Furthermore, the X-ray photoelectron spectroscopy data presented characteristic peaks of Fe, Ca, P, and O atoms in all samples. The Fourier Transform Infrared spectra displayed all the specific vibrations typical of the analyzed materials. What is more, the Vibrating Sample Magnetometer method confirmed the paramagnetic nature of the samples. It could be concluded that the synthesized composite has intermediate properties between the components used in the formation process. The results suggest that these composites are superparamagnetic. This type of material architecture would be well suited for biomedical applications.

Hydroxyapatite with magnetic core: Synthesis methods, properties, adsorption and medical applications.

This review presents the actual state of knowledge and recent research results on the magnetic composite synthesized from iron oxide (γ-Fe2O3 or Fe3O4) and hydroxyapatite. It can be obtained applying some methods, i.e. chemical precipitation, hydrothermal, sol-gel, and biomimetic or combined techniques which exhibit characteristic properties affecting the form of the prepared product. More specific details are discussed in this paper. A comparison of the discussed synthesis methods is presented. On the basis of selected publications, a comparison of the results of the analysis by XRD, FTIR, SEM and EDX methods for hydroxyapatite with a magnetic core was also presented. Moreover, the characteristics large adsorption capacity and specific area allow employing nanocomposites as adsorbents particularly in removal of toxic metal ions. Nowadays this issue is extremely vital due to large amounts of pollutants in the environment and greater ecological awareness of people. Moreover, magnetic hydroxyapatite can be also applied as a catalyst in various syntheses or oxidation reactions as well as in medicine in magnetic resonance imaging, hyperthermia treatment, drug delivery and release, bone regeneration or cell therapy.