Using Anticipatory Behavior to Detect the Change in Interest in an Activity Repeated Several Times and Avoid Habituation in Bottlenose Dolphins (Tursiops Truncatus).

Environmental enrichment is an essential component of the management policies used by zoos and aquaria to promote animals' welfare. However, when enrichments are provided several times, they can cause habituation and lose their enrichment effects. A simple method to avoid it could be making a preventive assessment about the trend of animals' interest in a stimulus provided several times. Here, we hypothesized that anticipatory behavior could be used to assess the decrease in interest in playing with objects when the activity is repeated. Moreover, we also assumed that this could be done before providing objects to play with. Our results support this hypothesis. Specifically, we found a positive correlation between the time the 7 dolphins tested spent performing anticipatory behavior before the enrichment sessions and the time they spent playing with objects during those sessions. As a result, anticipatory behavior performed before enrichment sessions predicted dolphins' interest in the session and allowed us to assess whether the sessions had lost their enrichment effect.

Benefit of Cognitive Environmental Enrichments on Social Tolerance and Play Behavior in Bottlenose Dolphins (Tursiops Truncatus).

Social tolerance is an essential feature of social life that can determine the good functioning of a group of animals. Play behaviors, like social play and playing with objects, are frequently associated with positive emotional and welfare states. As a result, zoos use various strategies to promote both social tolerance and play with objects. Providing animals with cognitive environmental enrichment can be an effective tool to achieve these goals. Here we tested whether cognitive environmental enrichment can promote social tolerance and play with objects in bottlenose dolphins (Tursiops truncatus). To this end, we provided a group of five dolphins with two types of cognitive enrichment: one for individual use and one for cooperative use, both based on the rope-pulling task paradigm. Then we evaluated whether social tolerance and play with objects had increased after we provided dolphins with the two enrichments. Our results go in this direction, showing that after we provided dolphins with the enrichments, their intolerance behaviors decreased, both during feeding sessions and play sessions, while their play with objects increased. As a result, the two enrichments we used could be useful for improving dolphins' housing conditions.

Cooperation increases bottlenose dolphins' (Tursiops truncatus) social affiliation.

Dolphins live in a fission-fusion society, where strong social bonds and alliances can last for decades. However, the mechanism that allows dolphins to form such strong social bonds is still unclear. Here, we hypothesized the existence of a positive feedback mechanism in which social affiliation promotes dolphins' cooperation, which in turn promotes their social affiliation. To test it, we stimulated the cooperation of the 11 dolphins studied by providing a cooperative enrichment tool based on a rope-pulling task to access a resource. Then we measured the social affiliation [simple ratio index (SRI)] of each possible pair of dolphins and evaluated whether it increased after cooperation. We also evaluated whether, before cooperation, pairs that cooperated had a higher SRI than those that did not cooperate. Our findings showed that the 11 cooperating pairs had significantly stronger social affiliation before cooperation than the 15 non-cooperating pairs. Furthermore, cooperating pairs significantly increased their social affiliation after cooperation, while non-cooperating pairs did not. As a result, our findings provide support to our hypothesis, and suggest that the previous social affiliation between dolphins facilitates cooperation, which in turn promotes their social affiliation.

Using Anticipatory Behavior as Preference Test in The Bottlenose Dolphin (Tursiops truncatus).

The preference tests have made a great contribution to promoting animal welfare. However, they are not always easily applicable and have some criticalities. Recently the anticipatory behavior has been used to detect the animals' preference. In this study, we attempted to investigate the relationship between preference and anticipatory behavior. To this end, we tested on a group of dolphins (Tursiops truncatus) whether, with anticipatory behavior, it is possible to distinguish the animals' preference for different objects and then use it as a preference test. Anticipatory behavior is exhibited throughout the animal kingdom, it is easy to induce and measure, and does not have the criticalities of other preference tests. Therefore, its use as preference test could facilitate the study of animals' preferences and contribute to the promotion of their welfare. Our results support the hypothesis of use anticipatory behavior as a preference test. In fact, dolphins have anticipated the arrival of objects highly preferred with a frequency three times higher than shown toward objects less preferred, providing further evidence of the correlation between anticipatory behavior and the animals' preference.

A fast technique to induce and measure anticipatory behavior in bottlenose dolphins (Tursiops truncatus).

Anticipatory behavior describes the actions taken to prepare for an upcoming event. It is considered a promising tool for welfare assessment, but it is not fully applied in zoos and other facilities. This is probably due to the need to train animals, which can take a long time and make its use impractical. In this work, we tested, on a group of six dolphins (Tursiops truncatus), the effectiveness of a new technique to induce and measure anticipatory behavior without training animals. Our results show how this new methodology, which we called The Fast Technique (TFT), has been able to induce and measure anticipatory behavior toward the event studied, i.e., the play sessions with objects (PSO). Furthermore, it has produced the same result obtained with The Classical Technique (TCT), which requires a training phase. Therefore, our finding demonstrates the efficacy of a new technique that could facilitate the use of anticipatory behavior for both research and animal welfare assessment protocols.

How to Minimize the Impact of Pandemic Events: Lessons From the COVID-19 Crisis.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the current pandemic of coronavirus disease 2019 (COVID-19). This pandemic is characterized by a high variability in death rate (defined as the ratio between the number of deaths and the total number of infected people) across world countries. Several possible explanations have been proposed, but it is not clear whether this variability is due to a single predominant factor or instead to multiple causes. Here we addressed this issue using multivariable regression analysis to test the impact of the following factors: the hospital stress (defined as the ratio between the number of infected cases and the total number of hospital beds), the population median age, and the quality of the National Health System (NHS). For this analysis, we chose countries of the world with over 3000 infected cases as of April 1, 2020. Hospital stress was found to be the crucial factor in explaining the variability of death rate, while the others had negligible relevance. Different procedures for quantifying cases of infection and death for COVID-19 could affect the variability in death rate across countries. We therefore applied the same statistical approach to Italy, which is divided into 20 Regions that share the same protocol for counting the outcomes of this pandemic. Correlation between hospital stress and death rate was even stronger than that observed for countries of the world. Based on our findings and the historical trend for the availability of hospital beds, we propose guidelines for policy-makers to properly manage future pandemics.