ABSTRACT
From the leaves and stem bark of Campylospermum flavum (Ochnaceae), three compounds, namely 4â´-O-methylagathisflavone, flavumchalcone, and flavumindole have been isolated together with 10 known compounds, including three flavonoids, two biflavonoids, two alkaloids, two nitrile glucosides, and glucopyranosyl-ß-sistosterol. The structures of these compounds and their relative configurations were established by 1D and 2D NMR experiments. The methanolic crude extracts of leaves and stem bark of C. flavum and compounds displayed a significant cytotoxicity towards Artemia salina larvae.
Subject(s)
Alkaloids/isolation & purification , Chalcones/isolation & purification , Cytotoxins/isolation & purification , Flavonoids/isolation & purification , Glucosides/isolation & purification , Indole Alkaloids/isolation & purification , Ochnaceae/chemistry , Alkaloids/chemistry , Alkaloids/pharmacology , Animals , Artemia/drug effects , Bacillus subtilis/drug effects , Cameroon , Candida albicans/drug effects , Chalcones/chemistry , Chalcones/pharmacology , Cytotoxins/chemistry , Cytotoxins/pharmacology , Escherichia coli/drug effects , Flavonoids/chemistry , Flavonoids/pharmacology , Glucosides/chemistry , Glucosides/pharmacology , Indole Alkaloids/chemistry , Indole Alkaloids/pharmacology , Larva/drug effects , Microbial Sensitivity Tests , Molecular Structure , Plant Bark/chemistry , Plant Leaves/chemistry , Staphylococcus aureus/drug effectsABSTRACT
Two nitrile glucosides (1S,3S,4S,5R)-4-benzoyloxy-2-cyanomethylene-3,5-dihydroxycyclohexyl-1-O-beta-glucopyranoside (campyloside A) and (1S,3S,4S,5R)-5-benzoyloxy-2-cyanomethylene-3-hydroxy-4-(2-pyrrolcarboxyloxy)cyclohexyl-1-O-beta-glucopyranoside (campyloside B) were isolated from the stem roots of Campylospermum glaucum, whereas serotobenine was isolated from Ouratea turnarea. The structure elucidations were based on spectroscopic evidence. The biological assays of compounds and crude extract of plant species showed good antimicrobial activity of crude extracts against Gram-positive cocci.
