[Sensitivity of Helicobacter pylori to antibacterial drug preparations in potential military recruits with duodenal peptic ulcer]. / Chutlyvist' Helicobacter pylori do antybakterial'nykh preparativ u pryzovnykiv, khvorykh na peptychnu vyrazku dvanadtsiatypaloï kyshky.

Results are submitted of bacteriological investigations in those persons with duodenal peptic ulcer who had been called up. The presence of regional strains of H. pylori is ascertained together with their sensitivity to certain antibacterial drug preparations. A rational antihelocobactertherapy schedule is proposed.

Specific identification of Mycobacterium bovis by monoclonal antibody-based enzyme immunoassay.

To improve identification of M. bovis, rabbit immune sera and mouse monoclonal antibodies against M. bovis-secreted protein antigens were used in the enzyme-linked assay (ELISA). In western blot analysis, M. bovis-specific epitopes within culture filtrate proteins were demonstrated to be mostly concentrated on the immunodominant 35-38 kDa antigen. Polyclonal and cross-reactive monoclonal antibodies were shown to be able to bind to all mycobacterial strains tested in ELISA (M. tuberculosis, M. bovis, M. kansasii, M. marinum, M. avium, M. smegmatis), but not to bacteria of the other genera. Among the monoclonal antibodies against M. bovis specific antigen, 2-6B was found to be the only one which could evidently react with whole cells of M. bovis in ELISA, but not with those of the other mycobacteria including M. tuberculosis.

[A chamber for microorganism microcultivation with microscopic monitoring]. / Kamera dlia mikrokul'tivirovaniia mikroorganizmov pod kontrolem mikroskopa.

A new chamber for microorganism microcultivation with microscopic monitoring has been developed and tried. It is not only fit for microcultivation of various microorganism species but is characterized by a number of advantages over the known chambers as well.

[A new method of cultivating Mycobacterium tuberculosis]. / Novyi metod kul'tivirovaniia mikobakterii tuberkuleza.

The possibility is shown of microcultivation of Mycobacteria tuberculosis on semipermeable cellophane membranes covering solid nutrients. The new method of cultivation may be used for enhanced microbiological diagnosis of tuberculosis, for determination of drug resistance of the tuberculous pathogen as well as for assessment of the biological properties of Mycobacteria tuberculosis in live unstained culture.

[Microscopic method of the determination of Mycobacterium tuberculosis sensitivity to chemotherapeutic agents]. / Mikroskopicheskii metod opredeleniia chuvstvitel'nosti mikobakterii tuberkuleza k khimioterapevticheskim preparatam.

A microscopic method of testing M. tuberculosis sensitivity to chemotherapeutic drugs was developed and tried out. According to it, M. tuberculosis are inoculated and cultivated on agar-agar gel disks, put on a Petri dish with an egg medium and chemotherapeutic drugs. Following the incubation lasting for 5-7 days at 37 degrees C, agar-agar gel disks with inoculated material are removed from the medium and subjected to microscopic examination as to confirm or deny the growth of mycobacteria in the form of microcolonies. The developed method is simple and easy to use, and makes it possible to shorten the period of testing M. tuberculosis sensitivity to chemotherapeutic drugs up to 5-7 days since the examination is started.

[Method of combined micro- and macrocultivation of Mycobacterium tuberculosis in egg medium using agar gel disks]. / Metodika sochetannogo mikro- i makrokul'tivirovaniia Mikobakterii tuberkuleza na iaichnykh sredakh s ispol'zovaniem diskov agarovogo gelia.

A procedure for combined micro- and macrocultivation of tubercle bacilli in the egg media by using agar gel disks was developed and experimentally tested. The essence of the procedure lies in plating mycobacteria on agar gel disks applied to the egg medium in Petri dishes followed by incubation at 37 degrees C for 5-7 days. Later on the agar gel disks are removed from the medium and examined microscopically for growth of tubercle bacilli in microcolonies (microcultivation). Then the gel disks are applied to the medium in the dishes from which they were removed and again incubated until macroscopically evident growth of tubercle bacilli is observed (macrocultivation).

[Experimental study of the antibiotic activity of cefuroxime]. / Eksperimental'noe izuchenie antibakterial'noi aktivnosti tsefuroksima.

The antimicrobial spectrum of cefuroxime, an antibiotic of the cephalosporin family was studied in vitro with respect to 11 species (16 strains) of gram-positive and gram-negative bacteria and in vivo on albino mice with experimental salmonellosis or pneumococcal infections. The bacteria were either test cultures or isolates from patients. The studies showed that cefuroxime had a wide antibacterial spectrum in vitro. It inhibited the growth of Staph. aureus, Str. pneumoniae, E. coli, Salm. typhimurium, Kl. pneumoniae, Bac. subtilis and had no effect on Ps. aeruginosa, Pr. vulgaris, M. tuberculosis and M. fortuitum. Cefuroxime had also a high bacteriostatic effect with respect to the experimental pneumococcal infection and a lower bacteriostatic effect with respect to the experimental salmonellosis infection.