ABSTRACT
One of the main issues in kidney transplantation is the optimal functional preservation of the organ until its transplantation into the appropriate recipient. Despite intensive efforts, the functional preservation period remains limited to hours. During this time, as a result of cellular injury, various proteins, peptides, and other molecules are released by the organ into the preservation medium. In this study, we used proteomic techniques to analyze the protein profiles of preservation solutions in which organs had been preserved prior to their transplantation. Samples were obtained from the preservation solutions of 25 deceased donor kidneys scheduled for transplantation. The protein profiles of the solutions were analyzed using 2D gel electrophoresis/MALDI-TOF and LC-MS/MS. We identified and quantified 206 proteins and peptides belonging to 139 different groups. Of these, 111 proteins groups were belonging to kidney tissues. This study used proteomic techniques to analyze the protein profiles of organ preservation solutions. These findings will contribute to the development of improved preservation solutions to effectively protect organs for transplantation.
Subject(s)
Kidney/metabolism , Organ Preservation Solutions/metabolism , Chromatography, Liquid/methods , Kidney Transplantation/methods , Organ Preservation/methods , Peptides/metabolism , Proteins/metabolism , Proteomics/methods , Tandem Mass Spectrometry/methodsABSTRACT
High Performance Liquid Chromatography LC-UV and LC-MS/MS methods were developed and validated for quantitative analyses of sodium benzoate and potassium sorbate in foods and beverages. HPLC-UV and LC-MS/MS methods were compared for quantitative analyses of sodium benzoate and potassium sorbate in a representative ketchup sample. Optimisation of the methods enabled the chromatographic separation of the analytes in less than 4 min. A correlation coefficient of 0.999 was achieved over the measured calibration range for both compounds and methods (HPLC and LC-MS/MS). The uncertainty values of sodium benzoate and potassium sorbate were found as 0.199 and 0.150 mg/L by HPLC and 0.072 and 0.044 mg/L by LC-MS/MS, respectively. Proficiency testing performance of Turkish accredited laboratories between the years 2005 and 2013 was evaluated and reported herein. The aim of the proficiency testing scheme was to evaluate the performance of the laboratories, analysing benzoate and sorbate in tomato ketchup.
Subject(s)
Beverages/analysis , Chromatography, High Pressure Liquid/methods , Food Preservatives/chemistry , Sodium Benzoate/analysis , Sorbic Acid/analysis , Tandem Mass Spectrometry/methods , Sodium Benzoate/chemistry , Sorbic Acid/chemistry , TurkeyABSTRACT
The compositions of colophony resins obtained from Pinus brutia Ten trees by three different methods (acid paste, carved hole and scraping) from Ayvacik, Gökova and Kemalpasa in Turkey were analyzed by capillary GC-MS. The main components were the monoterpenes alpha-pinene, beta-pinene, and delta3-carene, and the diterpenic resin acids palustric, abietic, kaur-9(11)-16-en-18-oic and neoabietic acid. The synthetic colophony resins exhibited similar contents to those of the natural resins obtained from the Gökova and Kemalpasa regions of Turkey. However, colophony resins from Ayvacik exhibited only half the diterpenic acid content as those of the Gökova and Kemalpasa resins. Out of the three techniques, the carved hole method caused rather different percentages in the constituents of the essential oils.
Subject(s)
Pinus/chemistry , Resins, Plant/chemistry , Abietanes/chemistry , Diterpenes/chemistry , Monoterpenes/chemistryABSTRACT
Atropine and obidoxime in a parenteral injection device are determined by simple HPLC method simultaneously without any pretreatment at 228 nm. The relative standard deviations (R.S.D.) were below 1.6% for the compounds. The correlation coefficient was greater than 0.999 for both compounds in the calibration range. The recoveries at 5 mg/L concentration averaged as 95% for atropine and 102% for obidoxime. The uncertainty of the measurements for atropine and obidoxime was 2.8% and 2.4%, respectively.
Subject(s)
Atropine/analysis , Chromatography, High Pressure Liquid/methods , Injections, Intraperitoneal/instrumentation , Obidoxime Chloride/analysis , Atropine/administration & dosage , Calibration , Obidoxime Chloride/administration & dosage , Reproducibility of Results , Sensitivity and Specificity , Spectrophotometry, UltravioletABSTRACT
The essential oil composition of Satureja thymbra was analyzed by direct thermal desorber and Headspace GC/MS analysis methods. Its constituents were determined to be mainly carvacrol (40.15%), gamma-terpinene (26.56%), p-cymene (16.39%), and thymol (13.16%). The other techniques, thermal desorber and Headspace GC/MS, were used for the plant leaves at three different temperature, which showed similar results. The thermal desorber GC/MS gave better and more sensitive results than Headspace GC/MS. The essential oil was found to be active against the bacteria Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Shigella sonnei, and Staphylococcus aureus and the yeast Candida albicans.
Subject(s)
Oils, Volatile/analysis , Oils, Volatile/pharmacology , Satureja/chemistry , Bacteria/drug effects , Candida albicans/drug effects , Gas Chromatography-Mass Spectrometry , Microbial Sensitivity Tests , Plant Leaves/chemistryABSTRACT
The water-distilled essential oil the leaves of Coridothymus capitatus were analyzed by GC/MS and also analyzed by direct thermal desorption GC/MS. Comparison was made between two analyses techniques. The essential oil consisted mainly of monoterpenes 98.9%, while oxygenated hydrocarbons were identified as 55.6 % and non-oxygenated hydrocarbons as 43.6%. As major components were found carvacrol (35.6%), p-cymene (21.0%), thymol (18.6%), gamma-terpinene (12.3%), alpha-terpinene (3.2%), beta-myrcene (3.0%) and alpha-thujene (1.3%) by hydrodistillation and by the GC/MS method. The direct thermal desorption GC/MS analysis also showed the same major components, namely carvacrol (51.6%), thymol (21.7%), p-cymene (9.7%) gamma-terpinene (8.2%), alpha-terpinene (1.64%). The essential oil of C. capitatus showed strong activity against S. aureus, P. vulgaris, P. aeruginosa, E. coli, K. pneumonia, B.
Subject(s)
Anti-Bacterial Agents/chemistry , Antifungal Agents/chemistry , Lamiaceae/chemistry , Oils, Volatile/chemistry , Plant Leaves/chemistry , Terpenes/chemistry , Anti-Bacterial Agents/pharmacology , Antifungal Agents/pharmacology , Bacteria/drug effects , Candida albicans/drug effects , Gas Chromatography-Mass Spectrometry , Microbial Sensitivity Tests , Oils, Volatile/isolation & purification , Oils, Volatile/pharmacology , Terpenes/isolation & purification , Terpenes/pharmacology , ThermodynamicsABSTRACT
The chemical composition of fatty acid methyl esters (FAMEs) from seeds of S. thymbra and S. cuneifolia were analyzed by GC/MS. 7 FAMEs were identified from the seeds of S. thymbra mainly as 9-octadecenoic acid methyl ester (43.9%), hexadecanoic acid methyl ester (11.4%), 9,12,15-octadecatrienoic acid methyl ester (Z,Z,Z) (30.2%), and octadecanoic acid methyl ester (14.1%), while from the seed of S. cuneifolia 10 FAMEs were obtained with the main components, similar to S. thymbra. These were identified as 9-octadecenoic acid methyl ester (10.1%), hexadecanoic acid methyl ester (methyl palmitate, 34.6%), 9,12,15-octadecatrienoic acid methyl ester (Z,Z,Z) (6.3%) and octadecanoic acid methyl ester (1.8%).
Subject(s)
Fatty Acids, Nonesterified/chemistry , Fatty Acids/analysis , Satureja/chemistry , Seeds/chemistry , Fatty Acids/chemistry , Fatty Acids, Nonesterified/isolation & purification , Gas Chromatography-Mass Spectrometry , Plant Leaves/chemistry , Satureja/classification , Species SpecificityABSTRACT
The composition of essential oil of the leaves of Lavandula stoechas ssp. stoechas, was analyzed by means of capillary GC-MS. The main components of L. stoechas ssp. stoechas oil were pulegone (40.4%), menthol (18.1%), menthone (12.6%). The essential oil of the plant was evaluated for antibacterial and a panel cytotoxic activities.
