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Nucleic Acids Res ; 16(22): 10751-64, 1988 Nov 25.
Article in English | MEDLINE | ID: mdl-2462715

ABSTRACT

The processed gene L32', a member of the mouse gene family for ribosomal protein L32, could encode a 135 amino acid protein nearly identical to L32. The 5'-flanking region of the gene contains CAAT and TATA sites at positions commonly found in expressed genes. The L32' gene lies within highly methylated, DNase I-insensitive chromatin of mouse L1210 cells. Although S1 nuclease digestion studies suggested that an L32' transcript might be produced, an oligonucleotide probe specific for L32' mRNA, and RNase digestion of a cRNA probe to L32', indicated fewer than 0.1 L32' transcripts/cell. These results demonstrate that extreme caution is required when measuring transcription from related genes.


Subject(s)
Escherichia coli/genetics , Genes, Bacterial , Genes , Multigene Family , Ribosomal Proteins/genetics , Transcription, Genetic , Amino Acid Sequence , Animals , Base Sequence , DNA, Bacterial/genetics , DNA, Bacterial/isolation & purification , Leukemia L1210/genetics , Mice , Molecular Sequence Data , Poly A/genetics , RNA/genetics , RNA, Messenger , Restriction Mapping
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