ASSESSMENT OF LABORATORY ANIMAL FUNCTIONAL STATUS: MODERN METHODOLOGICAL APPROACHES FOR CONDUCTING BIOMEDICAL RESEARCH.

OBJECTIVE: Aim: to outline modern scientific approaches for evaluating the functional condition of small laboratory animals in experimental research. PATIENTS AND METHODS: Materials and Methods: Scientific publications on the rules of using laboratory animals in scientific experiments, testing, educational process have been studied. The bioethical norms and principles of animal care and use of material for medical, veterinary and biological profiles were also studied, for example modern methodological approaches for conducting biomedical research, since it is the responsibility of the scientist to achieve reproducible research results using the minimum number of laboratory animals. CONCLUSION: Conclusions: The main conditions for their implementation and effectiveness include: adherence to the "Ethical Code" published by the International Council for Medical Scientific Organizations in the early 1980s, the principles of the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (Strasbourg, 18 March 1986), and Directive 2010/63/EU adopted on 22 September 2010; the use of advanced and modern diagnostic equipment that enables low-invasive yet highly informative research on small laboratory animals; conducting comprehensive, multi-level studies on an optimal number of animals in laboratories that meet international standards.

FEATURES OF PRENATAL MORPHOGENESIS AND PECULARITIES OF THE UTRICULUS PROSTATICUS FETAL ANATOMY.

OBJECTIVE: Aim: To determine the peculiarities of the structural organization of the utriculus prostaticus (UP) in pre-fetuses and fetuses. PATIENTS AND METHODS: Materials and methods: The study of macroscopic features and microscopic peculiarities of the prostate gland and the prostatic part of the urethra was carried out on 46 sections of human pre-fetuses and fetuses aged from 9 weeks to birth (31,0-375,0 mm PCL). The work uses the method of microscopic study of serial histological and topographic-anatomical specimens of the prostate gland, as well as the method of the thin preparation of the prostate part of the urethra in fetuses of different ages and morphometry. RESULTS: Results: In 58,0-66,0 mm PCL fetuses the paramesonephric ducts are reduced, except of their connected caudal part, which is a morphological substrate for the development of the UP. At 72,0-79,0 mm PCL fetuses, cavity is replaced by cellular mass. At the 85,0-120,0 mm PCL fetuses, the UP connects with the lumen of the urethra. The cavity of the UP intensivelly proliferates with cells. In fetuses of 125,0-135,0 mm PCL is presente dense arrangement of glandular elements, which are surrounded by fibrous-muscular membrane. In fetuses of 150,0-160,0 mm PCL, in the caudal direction, the cavity of the UP gradually narrows, it forms invaginations, especially in the middle and lower parts, or is divided into separate, interconnected chambers. In fetuses of 170,0-185,0 mm PCL, UP has elongated-oval or rounded-oval shape. In the caudal direction, the UP is directed ventral to the colliculus seminalis and is located slightly anterior and superior to the ejaculatory ducts. In 8-month-old fetuses, the lumen of the UP is lined with a pseudostratified cubical epithelium, outside of which there is a tunica muscularis. Ejaculatory ducts lined with a two-layer cuboidal epithelium are placed on both sides of the UP. A 270,0 mm PCL fetus has no UP at the apex of the colliculus seminalis. In fetuses 315,0-335,0 mm PCL, the process of cavity formation spreads to new areas of glandular formations of the prostate gland and their final branches. Most of the glandular formations open into the prostatic part of the urethra directly below the UP and the distal parts of the ejaculatory ducts. Microscopic examination of frontal sections of the prostate gland of a fetus with a 360,0 mm PCL revealed a septum in the UP which divides the cavity of the UP into the right and left halves of a round-oval shape. CONCLUSION: Conclusions: The formation of utriculus prostaticus occurs from the paramesonephric ducts in the 11th week of fetal development. At the beginning of the 4th month of intrauterine development, it gradually decreases in size. From the middle of the 5th month of prenatal development, the utriculus prostaticus lengthens, and starting from the fetus of 7 months, both its length and width increase. At the end of the fetal period, the utriculus prostaticus acquires a round-oval shape, its length increases from 0,5 to 4,3 mm during prenatal ontogeny.

THE PECULIARITIES OF MORPOLOGICAL CHANGES OF RATS' OVARY AND BIOCHEMICAL STATE UNDER THE DAMAGE WITH DIFFERENT DOSES OF LEAD ACETATE.

OBJECTIVE: The aim of the study was to study the effect of low and high doses of lead acetate on biochemical parameters and morphological status of rat ovaries in the experiment. PATIENTS AND METHODS: Materials and methods: The study was performed on 36 nonlinear female rats weighing 180-210 g, aged 4 months, divided into 3 experimental groups: I - control (C), II - rats, which were given 30 days to drink a solution of lead acetate with at the rate of 0,05 mg / kg of animal weight, group III - rats, which were given for 30 days to drink a solution of lead acetate at the rate of 60 mg/kg of animal weight. Biochemical research methods were included determination of diene conjugate concentration in animals' blood, concentration of TBA-active products, study of oxidative modification of proteins in blood plasma, determination of superoxide dismutase and catalase activities. Endogenous intoxication was assessed by the definition of medium-mass molecules, the content was expressed in units of extinction. The material for light microscopy investigation from the ovary was performed according to the generally accepted method. RESULTS: Results: Lead acetate causes activation of peroxidation of lipids and proteins in the body of female rats, which is directly dependent on the dose of lead. In response to the activation of free radical oxidation there are changes in the antioxidant system, which depend on the dose of lead acetate: at a dose of 0.05 mg / kg superoxide dismutase and catalase activity increase, at a dose of 60 mg / kg superoxide dismutase and catalase activity. Small doses of lead do not cause endogenous intoxication. Lead acetate causes the development of endogenous intoxication in animals only in large doses: increases the formation of toxic compounds, cell apoptosis, decreased excretory function of the kidneys, which is associated with multiorgan disorders. As a result of the action of lead acetate, morphological changes of the ovaries were observed, which increased with increasing dose of lead acetate. There was a dose-dependent decrease in massometric parameters, the number of follicles and changes in the thickness of the surface structures of the ovary, which is more pronounced at 60 mg/kg. CONCLUSION: Conclusions: Under the influence of small and large doses of lead acetate on biochemical changes in blood and morphological changes in the ovaries in male rats the oxidative stress is developed. Under the influence of small doses, the changes are adaptive, and under the influence of large doses - damaging.

VARIATIONS OF THE STRUCTURE, TOPOGRAPHY, BLOOD SUPPLY AND INNERVATION OF THE BRACHIORADIALIS IN HUMAN FETUS.

OBJECTIVE: The aim of the research was to establish the features of age-related and individual anatomical variability of the brachioradialis, its blood supply, and innervation options. PATIENTS AND METHODS: Materials and methods: The study of the variant anatomy of the brachioradialis and its vascular and nervous structures was carried out on 25 preparations of human fetuses of 4-7 months, 81.0-270.0 mm parietal-coccygeal length (PCL) using macromicroscopic preparation, injection vessels, and morphometry. RESULTS: Results: Spindle-like (56%) and round (24%) shapes of the brachioradialis were found in most of the studied fetuses; its elongated flat (12%) and triangular (8%) forms occur less often. In a fetus of 185.0 mm TKD, the right brachioradialis consisted of two separate parts: upper and lower, which were connected at the level of the middle of the forearm into a common short muscle belly. In another human fetus of 220.0 mm TKD, the right brachioradialis also consisted of two separate parts - upper and lower, triangular in shape, but separated by a pronounced horizontal gap. CONCLUSION: Conclusions: The features of the fetal anatomy of the brachioradialis are established: its variability and bilateral asymmetry of shape and size, variability of the places of origin and attachment, etc. In individual human fetuses, the brachioradialis consists of two separate parts that have special topographical relationships. The area of the greatest concentration of both extra- and intramuscular nerves and arteries is the upper and middle third of the brachioradialis. In the area of the forearm, the course of radial vascular-nerve formations is determined by the brachioradialis.