1.
J Clin Microbiol
; 41(4): 1763-5, 2003 Apr.
Article
in English
| MEDLINE
| ID: mdl-12682181
ABSTRACT
DNase I pretreatment of 16S rRNA gene PCR reagents was tested. The DNase I requirement for the elimination of false-positive results varied between 0.1 and 70 IU per master mix depending on the applied Taq polymerase. PCR sensitivity was mostly maintained when 0.1 IU of DNase I was used.