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1.
Int J Lepr Other Mycobact Dis ; 56(3): 443-8, 1988 Sep.
Article in English | MEDLINE | ID: mdl-3047285

ABSTRACT

Leprosy is the third leading cause of preventable blindness; however, little is known about the spread of infection to the eye. We have studied the eyes of three sooty managabey monkeys. Two were experimentally infected with Mycobacterium leprae; the third was not infected. In one of the infected animals there was histopathological evidence of lepromatous leprosy as evidenced by a chronic inflammatory infiltrate at the limbus, and detection of acid-fast bacilli in the corneal stroma, blood vessel walls, and corneal nerves. The latter were damaged as a result of the bacillary invasion. Electron microscopy revealed involvement and distortion of keratocytes with M. leprae and invasion of the corneal stroma by macrophages containing bacilli. Both infected animals showed focal collections of lymphocytes in the superficial stroma of the conjunctiva and in the ciliary body. This is the first report of the ocular manifestations of leprosy in any primate, including man, in which the duration of infection is known.


Subject(s)
Eye Diseases/pathology , Eye/pathology , Leprosy/pathology , Animals , Cercopithecidae , Cornea/microbiology , Cornea/pathology , Cornea/ultrastructure , Eye/microbiology , Eye/ultrastructure , Female , Macrophages/microbiology , Male , Microscopy, Electron , Microscopy, Electron, Scanning , Mycobacterium leprae/ultrastructure
2.
Int J Lepr Other Mycobact Dis ; 56(2): 302-9, 1988 Jun.
Article in English | MEDLINE | ID: mdl-3045226

ABSTRACT

Sixty lepromatous or borderline lepromatous patients were submitted to immunotherapy with a mixture of autoclaved Mycobacterium leprae and BCG. The histopathologic findings in skin biopsy specimens taken before and after immunotherapy were evaluated independently by six histopathologists in a workshop setting. Their pooled observations on diagnosis and classification were analyzed to assess the histopathologic changes following various periods of immunotherapy. Expressing the results as the average value of five to six independent observations, there were changes in classification of reversal or upgrading toward the tuberculoid end of the leprosy spectrum in 90.5% of the patients initially classified as lepromatous (LL), and in 83.3% of those initially classified as borderline lepromatous (BL). The histopathologic findings amply support the clinical, bacteriologic and immunological changes following immunotherapy from LL or BL, to BL, mid-borderline (BB) or even borderline tuberculoid (BT) leprosy.


Subject(s)
BCG Vaccine/therapeutic use , Bacterial Vaccines/therapeutic use , Immunotherapy , Leprosy/therapy , Mycobacterium leprae/immunology , Female , Humans , Leprosy/pathology , Male , Skin/pathology
3.
In. World Congress of Dermatology, 17; Orfanos, C. E; Stadler, R; Gollnick, H. World Congress of Dermatology, 17/Proceedings. Berlin, Spring Verlag, May 1988. p.535-8.
Non-conventional in English | Sec. Est. Saúde SP, HANSEN, Hanseníase Leprosy, SESSP-ILSLACERVO, Sec. Est. Saúde SP | ID: biblio-1245772

Subject(s)
Congress , Leprosy
4.
Int J Lepr Other Mycobact Dis ; 56(1): 61-5, 1988 Mar.
Article in English | MEDLINE | ID: mdl-3373087

ABSTRACT

The existence of naturally acquired leprosy in a second sooty mangabey monkey has been documented. The disease has the clinical and histopathological characteristics of subpolar lepromatous leprosy (LLs), and microbiological studies thus far confirm the etiologic agent as Mycobacterium leprae. This mangabey had been housed in direct contact with the first mangabey in which naturally acquired leprosy was diagnosed in 1979. Clinical symptoms appeared in the second mangabey in 1986, almost 7 years after the appearance of skin lesions in the first monkey. It is likely that the second mangabey contracted leprosy from the first mangabey or that both animals contracted the disease by contact with an unknown common third source. This is the only known possible natural transmission of leprosy from monkey to monkey, and suggests that a potential zoonosis exists in wild monkeys that may serve as a reservoir for the disease in areas where human leprosy is endemic.


Subject(s)
Cercopithecidae , Leprosy/veterinary , Monkey Diseases/transmission , Pregnancy Complications, Infectious/veterinary , Animals , Female , Leprosy/transmission , Pregnancy , Skin/pathology
5.
Acta Leprol ; 6(1): 51-60, 1988.
Article in English | MEDLINE | ID: mdl-3051854

ABSTRACT

Naturally-acquired leprosy has been reported in nine-banded armadillos captured in the southern United States, a chimpanzee from Sierra Leone, and in two "sooty" mangabey monkeys from Nigeria. A significant prevalence of leprosy in wild armadillos establishes this animal as a reservoir of M. leprae, and exposure to armadillos has been implicated as a source of leprosy in humans. Current evidence suggests that leprosy is a zoonosis in certain nonhuman primate species. Control and eradication programs for leprosy should take into consideration the possible influence of extra-human sources of M. leprae, especially zoonotic leprosy.


Subject(s)
Leprosy/veterinary , Zoonoses , Animals , Armadillos/microbiology , Cercopithecidae/microbiology , Humans , Leprosy/transmission , Monkey Diseases/transmission , Mycobacterium leprae/isolation & purification , Pan troglodytes/microbiology , United States
6.
Am J Trop Med Hyg ; 37(2): 385-91, 1987 Sep.
Article in English | MEDLINE | ID: mdl-3310689

ABSTRACT

Three African green monkeys (Cercopithecus aethiops) were inoculated intravenously and intracutaneously with Mycobacterium leprae derived from a naturally infected mangabey monkey. All developed cutaneous lesions at inoculation sites. One developed disseminated cutaneous lesions, while the cutaneous lesions in the other two regressed and eventually disappeared. The animals were examined at necropsy five years after inoculation. All three had active leprosy infection in peripheral nerves with extensive inflammation and fibrosis. The disease histologically resembled borderline-lepromatous leprosy. These findings add a new dimension to animal models of leprosy.


Subject(s)
Disease Models, Animal , Leprosy/microbiology , Monkey Diseases/microbiology , Peripheral Nerves/pathology , Animals , Cercopithecidae/microbiology , Chlorocebus aethiops/microbiology , Leprosy/pathology , Monkey Diseases/pathology , Mycobacterium leprae
7.
Int J Lepr Other Mycobact Dis ; 55(1): 109-15, 1987 Mar.
Article in English | MEDLINE | ID: mdl-3559334

ABSTRACT

A 6-month-old male rhesus monkey (Macaca mulatta) was inoculated intravenously and intracutaneously with Mycobacterium leprae obtained from a naturally infected mangabey monkey. The animal developed generalized lepromatous leprosy, and was killed for pathological examination 56 months after inoculation. Lesions were observed in the skin, nasal mucosa, peripheral nerves, and peripheral lymph nodes, with relative sparing of viscera. The monkey was carefully evaluated for the retrovirus STLV-III infection and was found negative. The rhesus monkey thus provides another animal model for the study of leprosy.


Subject(s)
Leprosy/pathology , Animals , Disease Models, Animal , Liver/pathology , Lymphatic System/pathology , Macaca mulatta , Male , Peripheral Nerves/pathology , Respiratory System/pathology , Skin/pathology
8.
J Leukoc Biol ; 40(5): 645-56, 1986 Nov.
Article in English | MEDLINE | ID: mdl-3534127

ABSTRACT

A decade has passed since our first report of naturally acquired leprosy in the nine-banded armadillo. Our studies and those of others during this period confirm the identification of the etiologic agent as Mycobacterium leprae. Confirmation is based on the results of histopathologic examination and microbiologic evaluations that included attempts to culture the organism, flourescent antibody studies, mycolic acid analysis, and DNA determinations demonstrating complete relatedness between the natural agent and M. leprae. Surveys involving large numbers of animals demonstrate a significant prevalence of the disease in armadillos captured in Louisiana and Texas. The discovery of naturally acquired leprosy in a chimpanzee in 1977 and a sooty mangabey monkey in 1979 reinforce the concept of leprosy as a zoonosis. Extensive contact with armadillos has been implicated by other observers in seven patients with leprosy in Texas. We believe the prevalence of leprosy in wild armadillos requires that they be considered a source of infection in patients from geographic areas where leprosy and armadillos co-exist.


Subject(s)
Armadillos/microbiology , Leprosy/veterinary , Xenarthra/microbiology , Animals , Leprosy/epidemiology , Leprosy/microbiology , Leprosy/pathology , Mycobacterium leprae , United States
9.
Int J Lepr Other Mycobact Dis ; 54(3): 427-36, 1986 Sep.
Article in English | MEDLINE | ID: mdl-3746044

ABSTRACT

The lepromin test was studied in rhesus monkeys. Six control monkeys which had not been inoculated with Mycobacterium leprae, six monkeys with experimentally induced leprosy, and nine monkeys which had been inoculated with M. leprae but had not developed leprosy were evaluated with 1X, 10X, and 15X lepromin A, with 1X and 10X lepromin M (mangabey monkey derived), with 1X and 25X purified inactivated M. leprae, and with an armadillo mock lepromin. We found that the lepromin test is useful in rhesus monkeys, but that a higher concentration of antigen than is used in humans is required to induce a response in monkeys. Control monkeys appear to be lepromin negative. Animals which have been inoculated and which develop lepromatous leprosy are also negative. Monkeys which are experimentally inoculated with M. leprae and do not develop leprosy become lepromin positive. Monkeys with indeterminate leprosy have reactions intermediate between lepromatous and resistant animals. No monkeys reacted to armadillo tissue. Our results indicate that 10X lepromin A is a useful preparation for the lepromin testing of rhesus monkeys.


Subject(s)
Lepromin , Leprosy/immunology , Animals , Disease Models, Animal , Female , Immunity, Cellular , Lepromin/immunology , Leprosy/pathology , Macaca mulatta , Male , Necrosis , Skin/pathology
10.
Clin Exp Immunol ; 65(2): 260-4, 1986 Aug.
Article in English | MEDLINE | ID: mdl-3491704

ABSTRACT

Leprosy in the mangabey monkey is an experimental model which is similar both clinically and histologically to human lepromatous leprosy. The immunopathology of these diseases was compared using monoclonal antibodies against T lymphocyte subpopulations in frozen tissue sections with an immunoperoxidase technique. In both mangabey and human lepromatous granulomas OKT4 (or Leu 3a) and Leu 2a cells were scattered among macrophages with greater numbers of Leu 2a as compared with OKT4 (or Leu 3a) cells. The results suggest that from an immunopathological standpoint experimental leprosy in mangabeys will provide a suitable model for the investigation of the pathogenesis of human lepromatous leprosy and for the evaluation of new antileprosy vaccines.


Subject(s)
Disease Models, Animal , Leprosy/immunology , T-Lymphocytes/classification , Animals , Cercopithecidae , Granuloma/etiology , Granuloma/immunology , Leprosy/complications , Male , Skin/immunology
11.
s.l; s.n; 1986. 5 p. tab.
Non-conventional in English | Sec. Est. Saúde SP, HANSEN, Hanseníase Leprosy, SESSP-ILSLACERVO, Sec. Est. Saúde SP | ID: biblio-1231447
13.
Int J Lepr Other Mycobact Dis ; 53(2): 269-77, 1985 Jun.
Article in English | MEDLINE | ID: mdl-4020216

ABSTRACT

A mangabey monkey (Cercocebus atys) was inoculated intravenously and intracutaneously with acid-fast bacilli (AFB) from a mangabey with spontaneously acquired leprosy. It developed generalized lepromatous leprosy and died 46 months after inoculation. Necropsy revealed severe lepromatous infiltrates in the skin, nasal mucosa, peripheral nerves, and testicles. Internal organs were only minimally involved. The lesions seen at necropsy were very similar to those seen in untreated cases of human lepromatous leprosy. These findings further substantiate the mangabey monkey as a suitable animal model for the study of lepromatous leprosy.


Subject(s)
Leprosy/pathology , Animals , Cercopithecidae , Male , Respiratory System/pathology , Skin/pathology , Urogenital System/pathology
14.
Int J Lepr Other Mycobact Dis ; 53(1): 1-14, 1985 Mar.
Article in English | MEDLINE | ID: mdl-3889184

ABSTRACT

Naturally acquired leprosy was detected in an otherwise normal "sooty" mangabey monkey (Cercocebus atys). This animal was imported from West Africa in 1975 and developed clinical symptoms of leprosy in 1979. Histopathologic findings were those of subpolar-lepromatous to borderline-lepromatous leprosy in the Ridley-Jopling classification. The disease was progressive, with crippling neuropathic deformities of the hands and feet. The disease regressed under specific therapy. The etiologic agent was identified as Mycobacterium leprae by the following criteria: invasion of nerves of host, staining properties, electron microscopic findings, noncultivable on mycobacteriologic media, DOPA-oxidase positive, lepromin reactivity, infection patterns in mice and armadillos, sensitivity to sulfone, and DNA homology. We believe the animal acquired the disease from a patient with active leprosy. The mangabey monkey offers promise as a primate model for leprosy, and adds a third reported species to animals with naturally acquired leprosy.


Subject(s)
Leprosy/veterinary , Monkey Diseases/pathology , Animals , Antibodies, Monoclonal/immunology , Biopsy , Blood Proteins/analysis , Cercopithecidae , Cytoplasm/ultrastructure , DNA/analysis , Female , Freeze Etching , Histiocytes/pathology , Immunoglobulins/analysis , Leprosy/immunology , Leprosy/pathology , Lymphocyte Activation , Mice , Mice, Inbred BALB C , Microscopy, Electron , Mitogens/pharmacology , Monkey Diseases/etiology , Monkey Diseases/immunology , Mycobacterium leprae/ultrastructure , Skin/pathology , T-Lymphocytes/classification , T-Lymphocytes/immunology
15.
Science ; 227(4686): 529-31, 1985 Feb 01.
Article in English | MEDLINE | ID: mdl-3917577

ABSTRACT

Eleven mangabey monkeys inoculated with Mycobacterium leprae developed lepromatous-type leprosy. Nine of the mangabeys were inoculated with M. leprae isolated from a mangabey with naturally acquired lepromatous leprosy. Immune function was depressed in some of these animals after dissemination of the disease. Two mangabeys developed lepromatous leprosy after inoculation with human M. leprae passaged in an armadillo. Three rhesus and three African green monkeys inoculated with mangabey-derived M. leprae also developed lepromatous leprosy. Mangabeys may be the first reported nonhuman primate model for the study of leprosy. Rhesus and African green monkeys may also prove to be reproducibly susceptible to the disease.


Subject(s)
Disease Models, Animal , Haplorhini , Leprosy , Animals , Antibodies, Bacterial/analysis , Cercopithecidae , Chlorocebus aethiops , Disease Susceptibility , Female , Leprosy/immunology , Leprosy/pathology , Leprosy/transmission , Lymphocyte Activation , Macaca mulatta , Male , Mycobacterium leprae/immunology , Saimiri , Species Specificity
16.
Cell Immunol ; 90(1): 115-30, 1985 Jan.
Article in English | MEDLINE | ID: mdl-3881186

ABSTRACT

Mononuclear cells from mangabey monkeys with disseminated experimental leprosy had increasingly severe depression of blastogenic responses to phytohemagglutinin, concanavalin A, and pokeweed mitogen as the disease progressed. Blastogenic responses were not depressed in cells from mangabeys with more localized disease. Blastogenic responses of cells from normal mangabeys appeared to vary with a circannual rhythm. The demonstration of significant negative correlations between the blastogenic responses to mitogens and the percentages of OKT8+ cells suggested that the mangabey OKT8+ subset may contain cells with suppressor function. The depressed responses to mitogens by cells from monkeys with disseminated experimental leprosy were associated with relatively high percentages of OKT8+ cells. Polyclonal immunoglobulin plaque-forming cell responses to pokeweed mitogen were depressed in cells from experimentally infected mangabeys. The results indicated that defects in immune regulation may occur in experimental leprosy in mangabeys, similar in some respects to the defects that have been reported in human leprosy.


Subject(s)
Leprosy/immunology , Monkey Diseases/immunology , Animals , Antibodies, Monoclonal/immunology , Antibody-Producing Cells/immunology , Cercopithecidae , Hemolytic Plaque Technique , Leprosy/transmission , Longitudinal Studies , Lymphocyte Activation , Lymphocytes/classification , Pokeweed Mitogens/pharmacology
17.
Int J Lepr Other Mycobact Dis ; 52(2): 198-202, 1984 Jun.
Article in English | MEDLINE | ID: mdl-6373628

ABSTRACT

Experimental leprosy lesions in the armadillo (Dasypus novemcinctus) were studied by freeze etching and ultrathin sectioning. Infected macrophages have distinct intracytoplasmic foamy structures in the form of spherical droplets accumulated around multiplying bacilli. This finding is the same as those observed in human lepra cells and nude mice macrophages infected with M. leprae.


Subject(s)
Leprosy/microbiology , Macrophages/ultrastructure , Mycobacterium leprae/growth & development , Animals , Armadillos , Cytoplasm/ultrastructure , Freeze Etching , Humans , Leprosy/pathology , Macrophages/microbiology , Mice , Mice, Nude
18.
Int J Lepr Other Mycobact Dis ; 52(2): 203-7, 1984 Jun.
Article in English | MEDLINE | ID: mdl-6373629

ABSTRACT

Ultrastructural features of the leproma of a) a naturally infected mangabey monkey, and lepromas and liver of b) a passage mangabey monkey, c) a rhesus monkey, d) an African green monkey, and e) a nine-banded armadillo inoculated with leprosy bacilli isolated from the leproma of a naturally infected mangabey monkey were studied by the freeze-etching technique. The size, shape, and ultrastructural features of leprosy bacilli in the phagolysosomes of macrophages in all of these samples were essentially the same as those in humans, nude mice, and armadillos inoculated with human Mycobacterium leprae. Distinct accumulations of small spherical droplets were observed around leprosy bacilli inside lepra cells of all the samples but were scarce in the specimen from the green monkey. The bacilli in all samples were long and slender, and had band structures on the smooth cell wall surfaces. The bacilli were indistinguishable from M. leprae.


Subject(s)
Leprosy/veterinary , Monkey Diseases/microbiology , Mycobacterium leprae/ultrastructure , Animals , Armadillos , Cebus , Chlorocebus aethiops , Freeze Etching , Leprosy/microbiology , Liver/ultrastructure , Macaca mulatta , Macrophages/ultrastructure , Monkey Diseases/pathology
20.
In. International Leprosy Congress, 12. International Leprosy Congress, 12/Proceedings. New Delhi, s.n, 1984. p.187-189.
Non-conventional in English | LILACS-Express | Sec. Est. Saúde SP, HANSEN, Hanseníase Leprosy, SESSP-ILSLACERVO, Sec. Est. Saúde SP | ID: biblio-1246389
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