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1.
Parasitol Int ; 50(4): 241-8, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11719110

ABSTRACT

The role of neutrophils in mediating host inflammation was examined in mice vaccinated with living third-stage infective hookworm larvae (L3). Mice were vaccinated by oral immunization with 500 L3 (Ancylostoma caninum) once every 2 weeks for a total of three immunizations. The vaccinated mice were then challenged intraperitoneally with 2000 L3) 1 week after the final immunization. To stimulate peritoneal production of neutrophils, 2 ml of 2% glycogen were injected intraperitoneally at 16 h prior to the challenge infection. Neutrophils were found to comprise 85% of the peritoneal cell population. L3 from the challenge infection were collected and then examined at timed intervals by inverted light microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Greater than a fivefold increase in the total numbers of peritoneal cells was noted in the vaccinated mice as compared to unvaccinated mice. In the peritoneal cavity of vaccinated mice, the neutrophils adhered to the L3 within 2 h, and over 55% of the L3 were surround by clusters of neutrophils to form a sausage-like sheath 4 h later. At 24-72 h after challenge, almost all of the L3 recovered from the vaccinated mice were covered with thick clusters of cells. Both SEM and TEM demonstrated extensive ultrastructural damage to the L3. In contrast, the L3 recovered from the unvaccinated mice appeared to be unaffected by neutrophils. These studies suggest that neutrophils, like macrophages, can have an important role as effector cells in L3-vaccinated mice.


Subject(s)
Ancylostoma/immunology , Ancylostomiasis/immunology , Neutrophils/immunology , Ancylostoma/ultrastructure , Ancylostomiasis/parasitology , Ancylostomiasis/prevention & control , Animals , Cell Adhesion , Male , Mice , Microscopy, Electron, Scanning , Neutrophils/parasitology , Peritoneal Cavity/parasitology , Peritoneal Cavity/pathology , Vaccination
2.
Acta Trop ; 76(2): 107-17, 2000 Sep 18.
Article in English | MEDLINE | ID: mdl-10936569

ABSTRACT

Praziquantel administered to the host causes damage to the tegument of Schistosoma mansoni. In this study, the effects of racemic praziquantel (Pra) and its enantiomers, levo-praziquantel (L-Pra) and dextro-praziquantel (D-Pra) were compared using scanning electron microscopy (SEM). Mice infected with S. mansoni for 49 days were treated with a single dose of Pra (300 mg/kg), L-Pra (150 mg/kg) or D-Pra (150 or 600 mg/kg). Groups of three mice were killed after 4 and 24 h, and schistosomes collected by perfusion and examined by SEM. Treatment with Pra or L-Pra, for 4 or 24 h, caused tegumental damage to S. mansoni including severe swelling, vacuolization, fusion of the tegumental ridges and loss or shortening of the spines on the tubercles, collapse and peeling. After treatment with D-Pra at 150 mg/kg, no apparent damage was observed. When the dosage was increased to 600 mg/kg, after 4 h lesions on the tegument similar to those induced by Pra or L-Pra were seen, but less severe. After 24 h, there was evidence of recovery. The study thus clearly showed that L-Pra was more active than D-Pra in causing tegumental damage. D-Pra showed a qualitatively similar activity at a higher concentration. It is possible that this effect was due at least to some extent to the small amount of L-Pra (<2%) which was present in the preparation of D-Pra used.


Subject(s)
Anthelmintics/pharmacology , Praziquantel/pharmacology , Schistosoma mansoni/drug effects , Schistosomiasis mansoni/drug therapy , Animals , Female , Male , Mice , Microscopy, Electron, Scanning , Optical Rotation , Schistosoma mansoni/ultrastructure , Schistosomiasis mansoni/blood , Stereoisomerism
3.
Acta Trop ; 75(3): 341-8, 2000 May 31.
Article in English | MEDLINE | ID: mdl-10838218

ABSTRACT

Alterations in the tegument of 21-day-old Schistosoma mansoni, caused by artemether administered to the infected mice, were studied using scanning electron microscopy (SEM). Mice were infected with S. mansoni cercariae, and after 21 days a single dose of artemether (400 mg/kg) was administered intragastrically. After 24, 72 h and 7 days groups of three mice were killed and the schistosomules collected by perfusion, fixed and processed routinely, and examined by SEM. After 24 h, all male and female worms examined showed alterations in the tegument, characterised by swelling, vesiculation and fusion of tegumental ridges; peeling, erosion and collapse of damaged tegumental surface, and also destruction of the oral sucker and acetabulum. After 72 h, severe damage to the tegument was seen, usually including extensive peeling, swelling and vesiculation, and host leukocytes were adhered to the damaged surface. Some worms were surrounded by clusters of host leukocytes or had even disintegrated. Seven days after treatment, some schistosomules still showed severe tegumental damage, but in some cases the damage was less than at earlier times, which suggested that those schistosomules that had survived were beginning to recover. The ability of artemether to cause severe damage to the tegument correlates with its high efficacy in killing 21-day-old schistosomules.


Subject(s)
Artemisinins , Schistosoma mansoni/drug effects , Schistosomiasis/drug therapy , Schistosomicides/therapeutic use , Sesquiterpenes/therapeutic use , Administration, Oral , Age Factors , Animals , Artemether , Female , Male , Mice , Microscopy, Electron, Scanning , Schistosoma mansoni/ultrastructure , Schistosomiasis/parasitology , Time Factors
4.
Acta Trop ; 71(2): 155-67, 1998 Sep 15.
Article in English | MEDLINE | ID: mdl-9821464

ABSTRACT

The role of peritoneal macrophages in a murine model of immunity to living hookworm third-stage larvae (L3) was investigated. Mice immunized orally with 500 L3 once every 2 weeks for three times were challenged intraperitoneally with 2000 L3 1 week after the final immunization. The challenged larvae were collected from the peritoneal cavity at intervals between 2 and 72 h and then examined by inverted light microscopy, scanning electron microscopy and transmission electron microscopy. Peritoneal cellular responses in non-immunized mice served as negative controls. The numbers of peritoneal macrophages in immunized mice were 6-7-fold higher than in non-immunized mice. In the peritoneal cavity of immunized mice, host macrophages adhered to the challenged L3 within 2 h and created a cocoon-like encasing which surrounded the parasite. Extensive damage to the L3 was observed which included swelling, collapse and deformation of the larval cuticle. Lysis and vacuolization of the parasite's internal structures were also observed. In contrast, no significant cellular adherence and damage were observed in L3 recovered from non-immunized mice. L3-specific antibody levels were also elevated in the peritoneum of immunized mice relative to non-immunized controls. These studies implicate macrophages as important effector cells in hookworm larval vaccine immunity.


Subject(s)
Ancylostoma/immunology , Ancylostomiasis/immunology , Macrophages, Peritoneal/immunology , Vaccination , Ancylostoma/growth & development , Ancylostoma/ultrastructure , Animals , Antibodies, Helminth/analysis , Antigens, Helminth/administration & dosage , Antigens, Helminth/immunology , Ascitic Fluid/immunology , Cell Adhesion , Enzyme-Linked Immunosorbent Assay , Immunity, Cellular , Immunoglobulin G/analysis , Larva/immunology , Larva/ultrastructure , Macrophages, Peritoneal/parasitology , Macrophages, Peritoneal/ultrastructure , Mice , Microscopy, Electron , Microscopy, Electron, Scanning
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