ABSTRACT
Serotype-specificity and sensitivity of oligonucleotide probes to serotype human rotaviruses was assessed. Probes could detect as little as 6.3 ng of homologous RNA and none reacted with as much as 100 ng of heterologous RNA. Northern-blot analysis revealed that probes reacted with one of genomic segments 7, 8 or 9 of corresponding serotypes.
Subject(s)
Diarrhea/microbiology , Feces/microbiology , RNA, Viral/analysis , Rotavirus/classification , Humans , Oligonucleotide Probes , Sensitivity and Specificity , SerotypingABSTRACT
Group A rotavirus strains from 3 locations in Bangladesh collected over one year were examined. Serotypes 1-4 were found throughout in 2 locations, whereas an epidemic of RV diarrhoea due to a single rotavirus strain occurred in one location.
Subject(s)
Rotavirus/isolation & purification , Adolescent , Adult , Aged , Bangladesh/epidemiology , Child , Child, Preschool , Diarrhea/epidemiology , Diarrhea/microbiology , Disease Outbreaks , Feces/microbiology , Humans , Infant , Infant, Newborn , Middle Aged , Rotavirus/classification , Rotavirus Infections/epidemiology , Rotavirus Infections/microbiology , Seasons , SerotypingABSTRACT
During an epidemiological study of human rotavirus infections in Bangladesh, three group A strains hybridized with a serotype 2 oligonucleotide probe, but they had long RNA electropherotypes. The three strains were collected from 8- to 20-month-old infants with acute diarrhea and moderate malnutrition. By a modified isolation procedure, two strains (T-B and T-C) were adapted in MA104 cell cultures. They were identified to be subgroup II specific by enzyme-linked immunosorbent assay with subgroup I- and II-specific monoclonal antibodies and were identified by a fluorescent focus reduction neutralization assay with hyperimmune antisera to be serotype 2 specific. Further characterization of these unusual rotavirus strains needs to be carried out.
Subject(s)
Antigens, Viral , Capsid Proteins , Rotavirus/classification , Antibodies, Viral , Capsid/genetics , Capsid/isolation & purification , Diarrhea/microbiology , Electrophoresis, Polyacrylamide Gel , Genes, Viral , Humans , Infant , RNA, Viral/isolation & purification , Rotavirus/genetics , Rotavirus/isolation & purification , Rotavirus Infections/microbiology , Serotyping , Virus CultivationABSTRACT
A study of neonatal group A rotavirus (RV) infection in 3 hospitals of urban Bangladesh identified 60 infected babies of 100 studied in 2 hospitals. Preliminary evidence based on serotyping and electropherotyping suggest that these RVs differ from community strains.
