Reliability, validity and sensitivity to change of neurogenic bowel dysfunction score in patients with spinal cord injury.

STUDY DESIGN: Psychometrics study. OBJECTIVES: The aim of this study is to investigate the validity, reliability and sensitivity to change of neurogenic bowel dysfunction (NBD) score. SETTING: Dokuz Eylül University Faculty of Medicine, Turkey. METHODS: The study included 42 patients with spinal cord injury (SCI). The reliability of NBD score was assessed by test-retest reliability and internal consistency. Cronbach's alpha coefficient was calculated to determine internal consistency. The construct validity was evaluated by exploring correlations between the NBD score and SF-36 scales, patient assessment of impact of NBD on quality of life (QoL) and the physician global assessment (PGA). The Global Rating of Change (GRC) scale was used to assess the change of NBD to investigate the sensitivity of the score to change. RESULTS: Cronbach's alpha coefficient was 0.547. In test-retest reliability analysis, high correlations between total test-retest NBD score and answers of each question were found (r=1.000, P<0.001). NBD score had a strong and significant correlation with PGA (r=0.98, P<0.000) and the impact on QoL (r=0.92, P<0.001). There was a significant negative correlation between NBD score and subscales of SF-36 (P<0.05) except physical functioning, physical role functioning and physical component summary score. There was a significant improvement in NBD scores after treatment (P=0.011). A significant positive correlation was found between GRC scale and change in total NBD score (r=0.821, P=0.007). CONCLUSION: The Turkish version of the NBD score is a valid and reliable instrument and also sensitive to change in patients with SCI.

Increased aflatoxin contamination of dried figs in a drought year.

Dried figs (4917 samples) destined for export from Turkey to the European Union were collected between September and December during the very dry crop year of 2007 and tested for aflatoxins B(1), B(2), G(1) and G(2) by immunoaffinity column clean-up and reverse-phase high-performance liquid chromatography (RP-HPLC). While 32% of the samples contained detectable levels of total aflatoxins, 9.8% of them exceeded the European Union limits. Aflatoxin levels were in the range of 0.2-259.46 microg kg(-1) and 2.04-259.46 microg kg(-1) for all samples and samples that exceeded the limits, respectively. A substantial increase in the incidence of aflatoxins was observed in 2007 compared with previous years, most likely due to the drought stress, high temperatures and low relative humidity encountered during the period from January to September of that year. In 2007, the mean temperature was 1-2 degrees C higher, there was 300 mm less total rain, and the mean relative humidity was 10-15% lower than in 2002-06. The average concentration of individual aflatoxins present in the samples was quantified to determine whether the drought conditions promoted certain types of aflatoxins. Among the contaminated samples, aflatoxin B(1) occurred in 97% of the contaminated samples, followed by G(1) in 47%, B(2) in 24%, and G(2) in 6% of samples. Concentrations of individual aflatoxins exhibited great variability among the samples but were not significantly different from those reported in previous studies, which were conducted under conditions without drought and high temperatures.

The dielectric properties of meats as a function of temperature and composition.

The dielectric properties of cod, perch, salmon, chicken breast, chicken thigh and beef were measured at 15 to 65 degrees C at 2450 MHz. The samples covered a moisture range of 68.9-81.2% and ash range of 0.96-1.20%. Equations were developed as a function of temperature, moisture, and ash, and compared to literature equations. The dielectric constant decreased with temperature and increased with moisture content. It was not affected by ash content. The dielectric loss factor increased with moisture content for moisture contents lower than 74.9%, then decreased for higher moisture contents. The dielectric-loss factor was quadratically related to temperature, decreasing to 30.2 degrees C then increasing. The dielectric loss factor increased with ash content. The effect of moisture content and temperature on the dielectric loss factor in the literature is reviewed to explain these results.

Use of dielectric properties to detect egg protein denaturation.

Changes in water and ion binding that occur during egg protein denaturation can be detected by measuring the dielectric properties. The dielectric properties of egg yolk, egg white and egg ovalbumin were tested from 25 to 105 degrees C at 11 frequencies from 300-2450 MHz. DSC was used to determine the temperature of protein denaturation. Both the dielectric constant and loss factor of egg yolk decreased due to denaturation of the protein lipovitellin. The dielectric constant increased at the initial denaturation and decreased after the complete denaturation and aggregation of egg ovalbumin in both egg white and ovalbumin.

Use of dielectric properties to detect whey protein denaturation.

Denaturation of whey proteins can be detected by the dielectric properties. The dielectric properties of 20% whey protein with 0, 5 or 15% sugar, 2% salt, at pH 4 and isolated whey proteins were measured from room temperature to 100 degrees C at 300-2450 MHz. The temperature at which a decrease in the dielectric loss factor, or increase in the dielectric constant, occurred was compared to the temperature of denaturation as determined by DSC and found to match. The change in the dielectric properties is likely caused by binding of water and/or ions that occurs during protein denaturation. For most of the samples, the dielectric loss factor showed the protein denaturation, but when ions were present due to salt or low pH, the dielectric constant showed the denaturation.