Grapevine morphological shade acclimation is mediated by light quality whereas hydraulic shade acclimation is mediated by light intensity.

Plants acclimate to shade by sensing light signals such as low photosynthetic active radiation (PAR), low blue light (BL) levels and low red-to-far red ratios (R:FR) trough plant photoreceptors cross talk. We previously demonstrated that grapevine is irresponsive to variations in R:FR and that BL-attenuation mediates morphological and architectural responses to shade increasing light interception and absorption efficiencies. However, we wondered if grapevine respond to low R:FR when BL is attenuated at the same time. Our objective was to evaluate if morphological, architectural and hydraulic acclimation to shade is mediated by low R:FR ratios and BL attenuation. To test this, we carried out experiments under natural radiation, manipulating light quality by selective sunlight exclusion and light supplementation. We grew grapevines under low PAR (LP) and four high PAR (HP) treatments: HP, HP plus FR supplementation (HP + FR), HP with BL attenuation (HP-B) and HP with BL attenuation plus FR supplementation (HP-B + FR). We found that plants grown under HP-B and HP-B + FR had similar morphological (stem and petiole length, leaf thickness and area), architectural (laminae' angles) and anatomical (stomatal density) traits than plants grown under LP. However, only LP plants presented lower stomata differentiation, lower δ13C and hence lower water use efficiency. Therefore, even under a BL and R:FR attenuated environment, morphological and architectural responses were modulated by BL but not by variation in R:FR. Meanwhile water relations were affected by PAR intensity but not by changes in light quality. Knowing grapevine responses to light quantity and quality are indispensable to adopt tools or design new cultural management practices that manipulate irradiance in the field intending to improve crop performance.

Toxicity and genotoxicity assessment in sediments from the Matanza-Riachuelo river basin (Argentina) under the influence of heavy metals and organic contaminants.

The aim of this study was to investigate the parameters of chemical extraction associated with the detection of toxicity and genotoxicity in sediment sample extracts. Quantitative analysis of metals and polycyclic aromatic hydrocarbons (PAHs), together with a battery of four bioassays, was performed in order to evaluate the extraction efficiency of inorganic and organic toxicants. The extracts were carried out using two inorganic solvents, two organic solvents and two extraction methodologies, making a total of five extracts. Two toxicity tests, the algal growth inhibition of Pseudokirchneriella subcapitata and the root elongation inhibition of Lactuca sativa, and two genotoxicity tests, the analysis of revertants of Salmonella typhimurium and the analysis of micronuclei and chromosomal aberrations in Allium cepa, were performed. According to the chemical analysis, the acidic solution extracted more heavy metal concentrations than distilled water, and dichloromethane extracted more but fewer concentrations of PAH compounds than methanol. Shaker extracts with distilled water were non-toxic to P. subcapitata, but were toxic to L. sativa. The acidic extracts were more toxic to P. subcapitata than to L. sativa. The methanolic organic extracts were more toxic to the alga than those obtained with dichloromethane. None of these extracts resulted toxic to L. sativa. Mutagenic effects were only detected in the organic dichloromethane extracts in the presence of metabolic activation. All the inorganic and organic extracts were genotoxic to A. cepa. This study showed that the implementation of different extraction methods together with a battery of bioassays could be suitable tools for detecting toxicity and genotoxicity in sediment samples.