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1.
Biol Sport ; 32(3): 249-54, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26424929

ABSTRACT

Sweat production is crucial for thermoregulation. However, sweating can be problematic for individuals with spinal cord injuries (SCI), as they display a blunting of sudomotor and vasomotor responses below the level of the injury. Sweat gland density and eccrine gland metabolism in SCI are not well understood. Consequently, this study examined sweat lactate (S-LA) (reflective of sweat gland metabolism), active sweat gland density (SGD), and sweat output per gland (S/G) in 7 SCI athletes and 8 able-bodied (AB) controls matched for arm ergometry VO2peak. A sweat collection device was positioned on the upper scapular and medial calf of each subject just prior to the beginning of the trial, with iodine sweat gland density patches positioned on the upper scapular and medial calf. Participants were tested on a ramp protocol (7 min per stage, 20 W increase per stage) in a common exercise environment (21±1°C, 45-65% relative humidity). An independent t-test revealed lower (p<0.05) SGD (upper scapular) for SCI (22.3 ±14.8 glands · cm(-2)) vs. AB. (41.0 ± 8.1 glands · cm(-2)). However, there was no significant difference for S/G between groups. S-LA was significantly greater (p<0.05) during the second exercise stage for SCI (11.5±10.9 mmol · l(-1)) vs. AB (26.8±11.07 mmol · l(-1)). These findings suggest that SCI athletes had less active sweat glands compared to the AB group, but the sweat response was similar (SLA, S/G) between AB and SCI athletes. The results suggest similar interglandular metabolic activity irrespective of overall sweat rate.

2.
Eur J Appl Physiol ; 112(12): 3971-82, 2012 Dec.
Article in English | MEDLINE | ID: mdl-22434252

ABSTRACT

This study examined hydration status, sweat losses, and the effects of flavoring and electrolytes on fluid intake for women (n = 27, age = 24 ± 4 years) walking at a self-selected pace for ~1 h on a 1 km outdoor path during summer mornings or evenings. Over five consecutive days, participants consumed ad libitum one non-caloric beverage containing: (1) water (W), (2) acidified water (AW), (3) acidified water with electrolytes (AWE), (4) acidified water with flavor (AWF), and (5) acidified water with flavor and electrolytes (AWFE) in a counter-balanced order during walks and a 1-h recovery period. Walk Wet bulb globe temperature (26.2 ± 1.8 °C) and pace (6.0 ± 0.5 km/h) did not differ among beverages (P > 0.05). Thirty-four percent of pre-walk urine specific gravity samples exceeded 1.020. Flavoring (AWF 700 ± 393 mL; AWFE 719 ± 405 mL) did not result in greater consumption (P > 0.05) over W (560 ± 315 mL), with all three beverages exceeding grand mean sweat losses (528 ± 208 mL). Addition of electrolytes did not influence (P > 0.05) the intake between AW versus AWE or AWF versus AWFE. The results of this study indicate that the majority of women will consume fluids in excess of their sweat losses within 1 h post-walk. Over half of consumption took place during walks, highlighting the importance of fluid availability during exercise. Great among-subjects variability in sweat losses and fluid intake support the need for promoting individualized hydration strategies based on the changes in body mass for athletic populations.


Subject(s)
Beverages , Drinking/physiology , Walking/physiology , Acids , Adult , Electrolytes , Female , Flavoring Agents , Humans , Specific Gravity , Sweat/metabolism , Urine/chemistry , Water
3.
J Sports Med Phys Fitness ; 50(3): 243-53, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20842083

ABSTRACT

AIM: This study investigated the effects of gender on repeated, maximal-intensity intermittent sprint exercise following variable day-to-day recovery periods. METHODS: Sixteen volunteers (8 men, 8 women) performed four trials of high-intensity intermittent sprint exercise consisting of three bouts of eight 30 m sprints (total of 24 sprints). Following completion of the baseline trial, in repeated-measures design, participants were assigned, in counter-balanced order, variable recovery periods of 24, 48, and 72 h whereupon they repeated an identical exercise trial. RESULTS: Results from a series of 4 (trial) x 3 (bout) repeated measures ANOVAs revealed men produced significantly (P < 0.01) faster times throughout all bouts and trials of repeated sprint exercise. Additionally, women exhibited significantly lower (P < 0.05) blood lactate concentration and significantly lower (P < 0.05) decrement in performance, indicating increased resistance to fatigue during repeated exercise sessions. There were no significant differences (P > 0.05) between genders for heart rate or rating of perceived exertion during or following trials. There were no significant differences for overall sprint performance within either gender among trials. CONCLUSION: These results indicate men, while able to produce higher absolute power outputs (i.e., lower sprint time), demonstrate higher decrement scores within a trial compared to women, thus suggesting women may recover faster and fatigue less. Also, gender differences affecting recovery within in a trial were observed to be diminished between trials (i.e., day-to-day recovery) of maximal intermittent sprint work evidenced by the observed stability of performance between trials following various recovery durations.


Subject(s)
Muscle Fatigue/physiology , Running/physiology , Analysis of Variance , Female , Humans , Lactates/blood , Male , Recovery of Function , Sex Factors , Young Adult
4.
Eur J Appl Physiol ; 109(1): 125-31, 2010 May.
Article in English | MEDLINE | ID: mdl-20135144

ABSTRACT

Athletes with spinal cord injury often experience high heat storage due to reduced sweating capacity below the spinal injury. Spray bottle (SB) may be used to apply mist for evaporative cooling during breaks in competitions. This study examined the efficacy of SB during rest breaks. Seven participants, four female and three males, (mean +/- SD age 24 +/- 4.1 year, weight 56.2 +/- 7.0 kg, upper-body VO(2) peak 2.4 +/- 0.6 l/min) volunteered for the study. Participants were paraplegic athletes (T3-T12/L1) with both complete and incomplete lesions. Participants arm-cranked using a ramp protocol in an environment of 21 +/- 1.5 degrees C and 55 +/- 3% rh once using a SB during 1-min rest between 7-min stages of increasing intensity and once without the SB (CON). Mean total work was similar (p = 0.86) for the SB and CON (2495.7 +/- 914.6 vs. 2407.1 +/- 982.3 kJ, respectively). Likewise, the mean work times were similar between trials (27 +/- 6 and 26 +/- 7 min for SB and CON, respectively). Furthermore, there were no significant differences detected between trials for skin temperature, rectal temperature, esophageal temperature (p > 0.05). There were no statistically significant differences detected between trials for RPE (p > 0.05). In conclusion, the application of artificial sweat via SB was ineffective in attenuating the onset of uncompensable heat strain during high-intensity arm exercise in a comfortable environment.


Subject(s)
Athletic Injuries/rehabilitation , Hypothermia, Induced/methods , Paraplegia/rehabilitation , Spinal Cord Injuries/rehabilitation , Sweat/physiology , Sweating , Adult , Athletic Injuries/complications , Athletic Injuries/physiopathology , Basketball , Female , Humans , Hypothermia, Induced/instrumentation , Male , Paraplegia/complications , Paraplegia/physiopathology , Skin Temperature , Spinal Cord Injuries/complications , Spinal Cord Injuries/physiopathology , Treatment Failure , Water/administration & dosage , Young Adult
5.
J Occup Environ Hyg ; 6(8): 455-9, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19412861

ABSTRACT

Ballistic protective helmets can impair heat dissipation. A cooling device in the helmet (cooling pad, CP) could help prevent heat problems in military personnel and potentially enhance comfort. This study examined the effects of CP on rectal and skin temperatures, heart rate, percent change in plasma volume, urine specific gravity, rating of perceived exertion, and other subjective measures while performing light work in a hot environment. It was hypothesized that the CP would act as an insulator to the head, which would not positively affect any physiological variable but could positively affect wearer subjective comfort or temperature. Participants performed a work protocol for approximately 2 hr. A ballistic vest, slacks, short-sleeved button-up shirt, and a ballistic helmet (one trial with CP and one trial without) were worn. Repeated measures analysis of variance (ANOVA) showed no differences (p > 0.05) between wearing and not wearing the CP for any physiological parameter. However, participants perceived the CP as cooler (p = 0.002). Other trends in perceptual data such as thermal strain and helmet comfort indicated the CP felt cooler. However, based on forehead temperature and participant comments, the CP lost its cooling ability relatively quickly (within approximately 30 min).


Subject(s)
Head Protective Devices , Military Personnel , Adult , Body Temperature , Body Temperature Regulation , Equipment Design , Heart Rate , Heat Stress Disorders/prevention & control , Humans , Male , Plasma Volume , Specific Gravity , Urine/chemistry
6.
Int J Sports Med ; 29(2): 139-44, 2008 Feb.
Article in English | MEDLINE | ID: mdl-17960503

ABSTRACT

Dehydration raises heat injury risk and reduces performance [ , , ]. The purpose was to validate the Hydra-Alert Jr (Acumen). The Hydra-Alert was tested in two exercise/clothing conditions. Participants wore it while wearing exercise clothing and exercising at a self-selected intensity (n = 8). Others wore the Hydra-Alert while wearing a ballistic-vest and performing an industrial-protocol (n = 8). For each condition, the Hydra-Alert was tested on two occasions (T1 and T2). The Hydra-Alert was tested against nude weight loss for both conditions. The Hydra-Alert had low test-retest reliability for both conditions (average absolute value of the error between Hydra-Alert outputs of T1 and T2 = 0.08 +/- 0.08 percentage points). With exercise-clothing, the Hydra-Alert evidenced low-moderate correlations between percent nude weight loss and Hydra-Alert output at 20 min (r = 0.59-T1, p = 0.13; r = 0.12-T2, p = 0.78), at 40 min (r = 0.93-T1, p = 0.001; r = 0.63-T2, p = 0.10), and at approximately 2 % weight loss (r = 0.21-T1 and T2, p = 0.61 and 0.62, respectively). The correlation at 40 min during T1 fell during T2 suggesting the Hydra-Alert was inconsistent. When wearing a ballistic-vest, the Hydra-Alert had poor validity (T1: r = - 0.29 [p = 0.48] for weight loss vs. monitor; T2: r = 0.11 [p = 0.80]). At the higher levels of dehydration ( approximately 2 %), the Hydra-Alert error was so high as to render its readings of little value. In some cases, the Hydra-Alert could lead to a false level of security if dehydrated. Therefore, the Hydra-Alert is of little use for those who want to measure their fluid loss while exercising in the heat.


Subject(s)
Dehydration/metabolism , Monitoring, Physiologic/instrumentation , Adult , Body Water/metabolism , Exercise Test/methods , Humans , Male , United States , Water-Electrolyte Balance/physiology
7.
Ergonomics ; 44(11): 953-61, 2001 Sep 15.
Article in English | MEDLINE | ID: mdl-11693246

ABSTRACT

Protective clothing (PC) results in a micro-environment between itself and the body. Workers are then exposed to a heat stress greater than the ambient environment alone, which is a reflection of micro-environment, metabolic rate and time. Adjustments to the ambient environment to account for the micro-environment have been formulated as a means to predict heat strain for safety and productivity purposes. Measurement of the actual micro-environment was made for a mean of 63.1 +/- 7.9 min using a remote sensor at the shoulder, hip and thigh levels on 15 subjects during a continuous work protocol (300 kcal/h) in impermeable PC at an ambient temperature of 30.1 degrees C wet bulb globe temperature (WBGT) (32 degrees C dry, 29 degrees C wet, 33 degrees C globe). Micro-environment temperature increased over the duration of the work period. There was no statistically significant difference (p>0.05) between the measurements made at the three different body sites for temperature or humidity. The mean micro-environmental WBGT at the end of work was 34.6 degrees C WBGT. Micro-environment WBGT increased rapidly in the first 20 min of work then slowed, rising only 0.5 degrees C WBGT from 40 to 60 min. These results suggest that at this particular high ambient temperature (30.1 degrees C WBGT) an adjustment factor of 5 degrees C WBGT would give a more accurate indication of thermal stress for up to 1 h of continuous moderate work within PC. For shorter work durations, an even smaller adjustment would be appropriate.


Subject(s)
Physical Exertion/physiology , Protective Clothing , Adult , Body Temperature/physiology , Heart Rate/physiology , Hot Temperature , Humans , Humidity , Male , Reference Values
8.
Ergonomics ; 44(11): 962-72, 2001 Sep 15.
Article in English | MEDLINE | ID: mdl-11693247

ABSTRACT

Personal heat strain monitoring provides the best means for maximizing worker safety and productivity in hot jobs. The present study compared methods for reducing the environmental effect on aural canal temperature in an attempt to reduce the predictive error of a personal heat stress monitor (QST) and a simple ear thermistor (EAR). Subjects underwent three exposures in impermeable protective clothing (PC) in an environment of 30.1 degrees C wet bulb globe temperature (WBGT) wearing either ear plugs (PLG), ear moulds (MLD) or ear moulds and earmuffs (MFS). Mean work time across all trials was 63.1 +/- 7.9 min, with a mean rectal temperature at 60 min of 38.5 +/- 0.1 degrees C and a sweat production rate of 29.7 +/- 8.6 g/min. Rectal temperature was used as the criterion measure of core temperature. Although group mean predictions were satisfactory, large SD of mean differences (+/- 0.36) meant that predictive ability of QST for individuals was greatly impaired. A simple well-insulated ear thermistor showed slightly better accuracy (+/- 0.28) as a predictor of rectal temperature across time, particularly at peak temperatures. Incorporating the predictive error of aural temperature still extended the duration of safe work times relative to ACGIH guidelines. Further investigation is still necessary to ensure that predictions based on aural temperature are accurate and safe for the worker population across varying conditions.


Subject(s)
Body Temperature/physiology , Physical Exertion/physiology , Thermometers , Adult , Ear Canal/physiology , Humans , Male , Predictive Value of Tests , Protective Clothing , Rectum/physiology , Reference Values
9.
J Sci Med Sport ; 4(2): 160-7, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11548915

ABSTRACT

This study examined subjects that exercised on three occasions in a heated environment (WBGT = 39 degrees C] until they experienced heat strain. Since morbidity and mortality due to heat injury increase with the duration of elevated core temperature, it is important that techniques to lower core temperature be evaluated. Following three exercise sessions, subjects underwent each of three core cooling treatments in random order: 1) Torso immersion in cool water, 2) Hands and feet immersion in cool water, and 3) Sit-in-shade with a 1.5mph breeze provided. Subjects (n=5) consistently reached peak rectal temperatures of 38.8 (+/-0.1) degrees C following each exercise bout in the heated environment. Torso immersion produced a significantly (p<0.05) greater rate of decline in rectal temperature (0.25+/-0.10 degrees C/min) than the hands and feet immersion technique (0.16+/-0.05 degrees C/min) and the sit in the shade technique (0.11+/-0.04 degrees C/min). After only 10 minutes of cooling, the differences among cooling techniques were evident. Similar trends were observed for mean heart rate readings, albeit not significant (p>0.05). It was concluded that rectal temperatures can be reduced rapidly through the use of a cool water torso-immersion technique.


Subject(s)
Body Temperature Regulation/physiology , Cryotherapy/methods , Exercise/physiology , Heat Stress Disorders/rehabilitation , Jogging/physiology , Abdomen , Adult , Foot , Hand , Humans , Immersion , Male , Rest/physiology , Thorax , Water
10.
Aviat Space Environ Med ; 72(5): 484-9, 2001 May.
Article in English | MEDLINE | ID: mdl-11346016

ABSTRACT

BACKGROUND: We previously reported that carbon dioxide (CO2) rapidly accumulates in the helmet of the NASA Launch and Entry Suit (LES) during a simulated egress from the Space Shuttle following 6 min of visor-closed seated rest to simulate approach and landing. The purpose of this study was to determine if CO2 accumulation and walking time in the LES would be improved by helmet visor-open rather than visor-closed seated rest prior to the performance of the simulated egress. METHODS: Wearing the LES, 12 male subjects performed 4 laboratory egress simulations consisting of 6-min seated rest, 2-min stand, and 5-min walk at 1.56 m x s(-1) (3.5 mph). During seated rest, subjects sat either with the visor open, breathing room air until the visor was closed on standing, or with the visor closed for the duration of the simulation. For all visor-closed operations 100%, O2 was supplied. The G-suit was either deflated (0.0 psi) or inflated to 1.5 psi. Inspired CO2 and walking time were measured. Data were analyzed at the end of seated rest, standing, and after 5 min of walking at 0.0 psi or after 2 min of walking at 1.5 psi (>90% of data available). RESULTS: Walk time was not different following visor-open (0.0 psi: 5.0 +/- 0.0; 1.5 psi: 3.4 +/- 0.3 min) or visor-closed (0.0 psi: 4.8 +/- 0.2; 1.5 psi: 3.5 +/- 0.4 min) seated rest at either G-suit pressure. Inspired CO2 levels were not different between the two conditions during walking at 5 min at 0.0 psi (p = 0.50; Open: 4.39 +/- 0.14; Closed: 4.48 +/- 0.18%) or at 2 min at 1.5 psi (p = 0.53; Open: 3.59 +/- 0.12; Closed: 3.65 + 0.21%). CONCLUSIONS: Visor position during seated rest immediately preceding the egress walk had no effect on inspired CO2 or walking time.


Subject(s)
Carbon Dioxide/analysis , Head Protective Devices , Space Suits , Walking , Adult , Analysis of Variance , Humans , Male , Posture
11.
Aviat Space Environ Med ; 72(2): 110-4, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11211038

ABSTRACT

BACKGROUND: In a previous study from our laboratory, we observed carbon dioxide (CO2) accumulation in the helmet of the NASA Launch and Entry Suit (LES) during a simulated emergency egress from the Space Shuttle. Of 12 subjects, 8 were unable to complete the egress simulation with a G-suit inflation pressure of 1.5 psi. The purpose of this report was to compare CO2 accumulation and egress walking time in the new Advanced Crew Escape Suit (ACES) with that in the LES. METHODS: Four male subjects who previously were unable to complete the egress in the LES performed a simulated egress while wearing the ACES with the G-suit inflated to 1.5 psi. The egress simulation consisted of 6 min of seated rest, 2 min of standing, and 5 min of walking on a treadmill at 1.56 m x s(-1) (3.5 mph) and 0% grade. The helmet visor was closed with the subjects receiving 100% oxygen throughout the simulation. Inspired CO2 and walking time were measured. RESULTS: The rate of CO2 accumulation was significantly less (ACES: 0.53 +/- 0.03, LES: 1.07 +/- 0.15 %CO2 x min(-1); p = 0.05) and walk time was greater in the ACES (ACES: 5.0 +/- 0.0, LES: 2.7 +/- 0.2 min; p = 0.002). CONCLUSIONS: Changes in the design of the ACES from the LES resulted in a decreased rate of CO2 accumulation and an improved egress walking time compared with the LES.


Subject(s)
Carbon Monoxide/analysis , Gravity Suits , Space Flight , Adult , Equipment Design , Equipment Failure , Humans , Hypergravity , Male , Task Performance and Analysis
12.
AIHAJ ; 61(5): 692-9, 2000.
Article in English | MEDLINE | ID: mdl-11071421

ABSTRACT

This study examined the effects of ingesting beverages of varying electrolyte-carbohydrate (ECHO) composition on hydration, sensory response, physiological function, and work performance during 4 hours of simulated industrial work for subjects wearing impermeable protective clothing (PC). Male subjects (N=18) completed four separate work sessions. Each session consisted of 30 min of treadmill walking with intermittent arm curls at 300 kcal per hour (moderate work rate), followed by 30 min of rest, for a total of 4 hours at 33 degrees C wet-bulb globe temperature. Excessive physiological strain prevented only four subjects from completing the 4-hour protocol. A different beverage was provided for consumption ad libitum for each work trial in a repeated measures, double-blind design. The beverages included lime colored water (W), lemon-lime placebo (P), lemon-lime ECHO with 18 mEq/L NaCl (ECHO18), and lemon-lime ECHO with 36 mEq/L NaCl (ECHO36). There was no difference in sweat production among the four trials (p = 0.61). Mean (standard deviation [SD]) fluid consumption was significantly greater for the ECHO36 [771 (+/-264) mL per hour] as compared with the W [630.6 (+/-234) mL per hour] and the P [655.2 (+/-228) mL per hour] (p<0.05), but not significantly greater than the ECHO18 [740.4 (+/-198) mL per hour]. Also, consumption of the ECHO18 was significantly greater than the W. Mean (SD) weight change, expressed as a percentage of total body weight (pre minus post), was -0.55(+/-0.8) for W, -0.31(+/-1.0) for P, -0.01(+/-1.1) for ECHO18, and +0.11(+/-1.1) for ECHO36 (p = 0.06). Subjects drank less and tended to experience greater weight loss in trials in which W or P were provided compared with trials in which either ECHO was provided. Thus, ECHO beverages, when provided ad libitum to workers wearing PC in a hot environment, produced better hydration than water.


Subject(s)
Carbohydrates , Fluid Therapy/methods , Rehydration Solutions/therapeutic use , Sodium Chloride , Adult , Body Temperature , Double-Blind Method , Exercise Test , Humans , Male , Physical Exertion , Sweating , Weight Loss
13.
Eur J Appl Physiol ; 82(3): 230-5, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10929217

ABSTRACT

Sweat rate may affect sweat lactate concentration. The current study examined potential gender differences in sweat lactate concentrations because of varying sweat rates. Males (n = 6) and females (n = 6) of similar age, percentage body fat, and maximal oxygen consumption (VO2max) completed constant load (CON) cycling (30 min--approximately 40% VO2max) and interval cycling (INT) (15 1-min intervals each separated by 1 min of rest) trials at 32 (1) degrees C wet bulb globe temperature (WBGT). Trials were preceded by 15 min of warm-up (0.5 kp, 60 rpms) and followed by 15 min of rest. Blood and sweat samples were collected at 15, 25, 35, 45, and 60 min during each trial. Total body water loss was used to calculate sweat rate. Blood lactate concentrations (CON approximately equal to 2 mmol.l-1, INT approximately equal to 6 mmol.l-1) and sweat lactate concentrations (CON and INT approximately equal to 12 mmol.l-1) were not significantly different (P > 0.05) at any time between genders for CON or INT. Overall sweat rates (ml.h-1) were not significantly different (P > 0.05) between trials but were significantly greater (P < or = 0.05) for males than for females for CON [779.7 (292.6) versus 450.3 (84.6) ml.h-1] and INT [798.0 (268.3) versus 503.0 (41.4) ml.h-1]. However, correcting for surface area diminished the difference [CON: 390.7 (134.4) versus 277.7 (44.4) ml.h-1, INT: 401.5 (124.1) versus 310.6 (23.4) ml.h-1 (P < or = 0.07)]. Estimated total lactate secretion was significantly greater (P < or = 0.05) in males for CON and INT. Results suggest that sweat rate differences do not affect sweat lactate concentrations between genders.


Subject(s)
Lactic Acid/analysis , Sex Characteristics , Sweat/chemistry , Adult , Bicycling , Female , Humans , Lactic Acid/blood , Male , Osmolar Concentration , Physical Fitness , Sweating
14.
Int J Sports Med ; 21(8): 556-60, 2000 Nov.
Article in English | MEDLINE | ID: mdl-11156274

ABSTRACT

Sweat lactate results from eccrine gland metabolism, however, the possible clearance of blood lactate through sweat has not been resolved. On separate days in an environmental chamber (32 +/- 1 C) 12 subjects completed a constant load (CON) (30 min at 40% VO2 max) and an interval cycling trial (INT) (15 one-min intervals at 80% VO2 max, each separated by one min rest) each designed to elicit different blood lactate responses. Each 30 min cycling trial was preceded by 15 min warm-up (30 watts) and followed by 15 min passive rest. Sweat and blood were analyzed for lactate concentration at 15, 25, 35, 45, and 60 min during CON and INT. Total body water loss was used to calculate sweat rate (ml/hr). Blood lactate was significantly greater (p < or = 0.05) at 25, 35, 45, and 60 min during INT compared to CON (approximately 5 mmol/L vs 1.5 mmol/L). Sweat lactate was not significantly different (p>0.05) between trials at any time (approximately 10 mmol/L). Sweat rates (approximately 600ml/hr) and estimated total lactate secretion were not significantly different (CON vs. INT) (p > 0.05). Elevated blood lactate was not associated with changes in sweat lactate concentration. Sweat lactate seems to originate in eccrine glands independent of blood lactate.


Subject(s)
Exercise/physiology , Lactic Acid/blood , Sweat/chemistry , Adult , Eccrine Glands/physiology , Female , Humans , Male
15.
J Med Microbiol ; 48(11): 1017-1022, 1999 Nov.
Article in English | MEDLINE | ID: mdl-10535646

ABSTRACT

A specific 16S rDNA PCR and subsequent hybridisation reaction was designed to discriminate between strains of Prevotella intermedia (n = 15) and P. nigrescens (n = 15). This technique was then used to detect the presence of these two bacterial species in acute suppurative oral infection. A total of 36 pus samples aspirated from 26 peri-apical abscesses, three root canals, three periodontal abscesses, two cases of refractory periodontitis, one cyst and one haematoma was examined. A portion of the pus sample was processed by PCR and the remainder of the specimen was subjected to routine culture. The PCR-based technique gave an identical pattern of detection of P. intermedia or P. nigrescens to that obtained by culture for 30 of the 36 specimens. Either P. intermedia or P. nigrescens was present in 14 samples and neither species was detected in 16 samples. In the remaining six samples the PCR method indicated the presence of one (n = 3) or both (n = 3) of the Prevotella species but neither or only one species was isolated by culture. It is concluded that the presence of P. intermedia and P. nigrescens in pus can be detected rapidly and specifically by direct PCR amplification of 16S rDNA. P. nigrescens was detected more frequently than P. intermedia in suppurative peri-apical infection both by culture and PCR.


Subject(s)
Bacteroidaceae Infections/microbiology , DNA, Ribosomal/analysis , Periodontitis/microbiology , Prevotella intermedia/isolation & purification , Prevotella/isolation & purification , RNA, Ribosomal, 16S/genetics , Bacteroidaceae Infections/diagnosis , DNA Primers/chemistry , DNA, Bacterial/analysis , Humans , Nucleic Acid Hybridization , Periapical Abscess/diagnosis , Periapical Abscess/microbiology , Periodontal Abscess/diagnosis , Periodontal Abscess/microbiology , Periodontitis/diagnosis , Polymerase Chain Reaction , Prevotella/genetics , Prevotella intermedia/genetics , Pulpitis/diagnosis , Pulpitis/microbiology , RNA, Bacterial/genetics , Reproducibility of Results , Suppuration/microbiology
16.
Am Ind Hyg Assoc J ; 60(4): 495-501, 1999.
Article in English | MEDLINE | ID: mdl-10462783

ABSTRACT

Chemical protective clothing (PC) use while working results in elevated rectal temperatures (Tre) that limit work time. Particle barrier, vapor permeable (PBVP) PCs allow workers to cool themselves by evaporating some sweat. The purpose of this study was to compare the effects on worker productivity of two types of PBVP suits, a Kleenguard (PPPC) (Kimberly Clark), and a Tyvek (PEPC) (DuPont) suit. Fifteen males in a repeated measures design performed four work tests consisting of a walk/arm curl combination at a time-weighted work rate of 1.0 L/min (300 kcal/hr), two in a wet bulb globe temperature (WBGT) of 26 degrees C and two in a WBGT of 18 degrees C, with subjects wearing each suit once in each environment. No significant difference (p > 0.05) was observed between the suits at 18 degrees C WBGT, but a significant difference was found (p < 0.05) between the suits, with the PPPC having a lower Tre in the WBGT = 26 degrees C at the 80th, 100th, and 120th min. A significant difference (p < .05) was also seen in the 26 degrees C WBGT with the PPPC resulting in a lower heart rate (HR) at the 40th, 60th, 80th, 100th, and 120th min and rate of perceived exertion (RPE) at the 75th, 90th, and 120th min. Additionally, a significant difference (p < .05) was seen between PEPC and PPPC for Tre, delta Tre, mean skin temp (mTsk), delta mTsk, and HR, each regressed against time in the 26 degrees C WBGT. Twelve of the 15 subjects also reported feeling cooler in the PPPC versus the PEPC in either WBGT environment.


Subject(s)
Exercise/physiology , Protective Clothing/standards , Adolescent , Adult , Analysis of Variance , Body Temperature/physiology , Exercise Test , Humans , Male , Occupational Exposure/prevention & control , Permeability , Reference Values , Sweating/physiology
17.
Aviat Space Environ Med ; 70(7): 656-65, 1999 Jul.
Article in English | MEDLINE | ID: mdl-10417001

ABSTRACT

BACKGROUND: In the event of an emergency on landing, Space Shuttle crewmembers while wearing the Launch and Entry Suit (LES) must stand, move to the hatch, exit the spacecraft with the helmet visor closed breathing 100% O2, and walk or run unassisted to a distance of 380 m upwind from the vehicle. The purpose of this study was to characterize the inspired CO2 and metabolic requirements during a simulated unaided egress from the Space Shuttle in healthy subjects wearing the LES. METHODS: As a simulation of a Shuttle landing with an unaided egress, 12 male subjects completed a 6-min seated pre-breathe with 100% O2 followed by a 2-min stand and 5-min walking at 1.56 m x s(-1) (5.6 km x h(-1), 3.5 mph) with the helmet visor closed. During walks with four different G-suit pressures (0.0, 0.5, 1.0, 1.5 psi; 3.4, 6.9, 10.3 kPa), inspired CO2 and walking time were measured. After a 10-min seated recovery, subjects repeated the 5-min walk with the same G-suit pressure and the helmet visor open for the measurement of metabolic rate (VO2). RESULTS: When G-suit inflation levels were 1.0 or 1.5 psi, only one-third of our subjects were able to complete the 5-min visor-closed walk after a 6-min pre-breathe. Inspired CO2 levels measured at the mouth were routinely greater than 4% (30 mmHg) during walking. The metabolic cost at the 1.5 psi G-suit inflation was over 135% of the metabolic cost at 0.0 psi inflation. CONCLUSION: During unaided egress, G-suit inflation pressures of 1.0 and 1.5 psi resulted in elevated CO2 in the LES helmet and increased metabolic cost of walking, both of which may impact unaided egress performance. Neither the LES, the LES helmet, nor the G-suit were designed for ambulation. Data from this investigation suggests that adapting flight equipment for uses other than those for which it was originally designed can result in unforeseen problems.


Subject(s)
Carbon Dioxide/analysis , Carbon Dioxide/metabolism , Energy Metabolism/physiology , Space Suits/adverse effects , Walking/physiology , Adult , Breath Tests , Emergencies , Equipment Design , Ergonomics , Evaluation Studies as Topic , Exercise Test , Humans , Male , Pressure , Space Flight , Time Factors , United States , United States National Aeronautics and Space Administration
18.
Am Ind Hyg Assoc J ; 60(1): 96-104, 1999.
Article in English | MEDLINE | ID: mdl-10028621

ABSTRACT

This study tested a new ice cooling system that permits ice cooling system recharge without personal protective clothing removal. Six male volunteers (22.1 +/- 1.2 years) underwent tests with the new ice cooling system (COOL) and without (NOCL) at a moderate work rate (450 W) in three environments of 28, 23, and 18 +/- 1 degrees C wet bulb globe temperature. Walks at 28 degrees C were carried out first with NOCL and COOL counterbalanced, then test order and environment were counterbalanced. At 28 degrees C, mean work time in COOL significantly increased by 37.5 min (188%) over NOCL (p < 0.05). At 23 degrees C mean work time in COOL was significantly increased by 44.3 min (171%) compared with NOCL (p < 0.05). Mean work times at 18 degrees C were not significantly different, although all subjects completed the 120 minutes of work in COOL compared with a mean work time of 109 +/- 20 min for NOCL. During rest, mean reductions in rectal temperature were significantly greater in COOL than NOCL (p < 0.05) at 28 and 23 degrees C. Mean heart rate calculated for the same point in both treatments was significantly lower for COOL at 28, 23, and 18 degrees C (p < 0.05). Thermal comfort rating was significantly different at 18 and 23 degrees C (p < 0.05). This new design seemed to provide comparable cooling to conventional vests and also provides greater practicality for field use. Even in experimental form the suit demonstrated increased productivity due to extended tolerance time.


Subject(s)
Heat Stress Disorders/prevention & control , Ice , Occupational Diseases/prevention & control , Protective Clothing , Adult , Analysis of Variance , Body Temperature/physiology , Cold Temperature , Heart Rate/physiology , Humans , Male , Oxygen Consumption , Work
19.
Mil Med ; 164(12): 891-6, 1999 Dec.
Article in English | MEDLINE | ID: mdl-10628164

ABSTRACT

Obstacle courses (OCs), physical challenge courses, and confidence courses are valuable in training and assessing military troops. However, OCs are not well characterized with regard to physical demands and requisite abilities. The purpose of this study was to evaluate the physical capabilities associated with success on an OC. Male subjects (N = 47) were assessed on an OC, skinfolds, upper and lower body aerobic and anaerobic power, muscular strength, and endurance. Faster performers were lighter (p < 0.003), leaner, and, relative to body weight, averaged greater arm anaerobic peak and mean power, leg aerobic power, one-repetition maximum leg press, and one-repetition maximum latissimus dorsi pull-down, than slower performers. There were significant correlations between OC time and weight (0.59), percent fat (0.54), anaerobic leg mean power (-0.43), arm anaerobic peak (-0.48) and mean power (-0.48), and arm (-0.51) and leg aerobic power (-0.53), all expressed relative to body weight. A three-variable regression model accounted for 35% of the variation in OC time. Good performers on this OC displayed many diverse physical capabilities.


Subject(s)
Military Personnel , Physical Education and Training , Physical Fitness/physiology , Adult , Body Composition/physiology , Humans , Male , United States
20.
Am Ind Hyg Assoc J ; 60(6): 747-51, 1999.
Article in English | MEDLINE | ID: mdl-10635540

ABSTRACT

This study examined fluid intake, weight changes, and palatability of water and a carbohydrate-electrolyte (ECHO) beverage in two groups of eight subjects performing 4 hours of simulated industrial work while wearing impermeable protective clothing. Subjects also rated the palatability of a flavorless orange-colored water and four commercially available orange-flavored fluid-replacement drinks. Subjects worked 30 min at 300 Kcal/hour (moderate work rate), followed by 30 min of rest for a total of 4 hours in each of three environments: 18, 23, 27 degrees C wet bulb globe temperature (WBGT). Eight subjects drank water and eight drank an ECHO beverage (Drink 1). Mean sweat production was similar between groups at each WBGT, (p > 0.05). Mean weight changes expressed as percentage of total body weight for the water-drinking group were -0.25 (+/- 0.16) kg in 18 degrees C, -0.55 (+/- 0.09) kg in 23 degrees C, and -0.93 (+/- 0.13) kg in 27 degrees C. Mean weight changes for the ECHO-drinking group were 0.49 (+/- 0.12) kg in 18 degrees C, 0.13 (+/- 0.12) kg in 23 degrees C, and -0.02 (+/- 0.14) kg in 27 degrees C. Each change was significantly different from that of the water-drinking group at the same temperatures (p < 0.05). Mean pre- to post-trial palatability rating results for all temperatures revealed differences in taste perception with strong preferences for Drinks 1 and 2 (pre to post): Drink 1, 3.8 to 3.6; Drink 2, 3.8 to 3.7; Drink 3, 3.0 to 2.7, Drink 4 (water), 3.2 to 3.0, and Drink 5, 2.1 to 1.6. Drinks 1 and 2 were rated significantly more palatable (p < 0.05) than Drink 3. All drinks had a reduced flavor appeal from 3.18 (+0.96) preratings to 2.92 (+1.01) postratings (p < 0.05). Mean voluntary consumption during work across the three temperatures was 1561.5 mL (+/- 720.1) for ECHO versus 1062.7 mL (+/- 666.4) for water (p = 0.054). The water group experienced greater weight loss than the ECHO group, suggesting a greater rate of dehydration when water was used for fluid replacement.


Subject(s)
Dehydration/therapy , Fluid Therapy , Hot Temperature/adverse effects , Occupational Diseases/therapy , Occupational Exposure/adverse effects , Rehydration Solutions/standards , Adult , Analysis of Variance , Body Temperature Regulation , Dehydration/etiology , Dehydration/physiopathology , Drinking , Humans , Male , Occupational Diseases/etiology , Occupational Diseases/physiopathology , Occupational Health , Protective Clothing/adverse effects , Sweating , Taste , Weight Loss
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