ABSTRACT
Pyrostegia venusta (Ker Gawl.) Miers, Bignoniaceae, is native to the Brazilian Cerrado and popularly known as "cipó-de-são-joão". In Brazilian folk medicine, the flowers of P. venusta are used as a general tonic and a treatment for diarrhea, vitiligo, cough, and common infections and inflammatory diseases of the respiratory system. Nevertheless, there are still no studies on its possible anti-inflammatory and antinociceptive effects. The P. venusta hydroethanolic extract (PvHE) was used to evaluate the anti-inflammatory and analgesic effects in carrageenan-induced paw edema, peritonitis induced by lipopolysaccharide, acetic acid-induced writhing, and formalin-induced paw-licking tests in Swiss male mice. PvHE at doses of 30-300 mg/kg p.o. demonstrated anti-inflammatory effect. PvHE reduced paw edema induced by carrageenan and inhibited leukocyte recruitment into the peritoneal cavity. The extracts showed antinociceptive activity in acetic acid-induced writhing and formalin tests. Our results showed that the PvHE demonstrated anti-inflammatory and antinociceptive action in mice. All the anti-inflammatory actions obtained are also suggested to due the presence of acacetin-7-O-β-glucopyranoside.
ABSTRACT
AIM OF THE STUDY: Pyrostegia venusta (Ker.) Miers (Bignoniaceae) is native to the Brazilian Cerrado and popularly known as "cipó-de-são-joão." In traditional Brazilian medicine, the Pyrostegia venusta are used as a general tonic as well as a treatment for diarrhea, vitiligo, cough, and common diseases of the respiratory system related to infections, such as bronchitis, flu and cold. This study was conducted to evaluate the effects of a hydroethanolic extract of flowers of Pyrostegia venusta on sickness behaviors induced by lipopolysaccharide in mice. MATERIALS AND METHODS: To evaluate the effects of orally administered Pyrostegia venusta hydroethanolic extract (PvHE) on lipopolysaccharide-induced sickness behaviors, mice were submitted to the forced swim test (FST) and the open field test. RESULTS: Lipopolysaccharide (LPS, 100 µg/kg, i.p.) administration increased the time spent floating in the FST and depressed locomotor activity in the open field. Pretreatment with PvHE at test doses of 100 and 300 mg/kg, p.o. attenuated the behavioral changes induced by LPS in the FST and open field test. This effect was similar to pretreatment with dexamethasone (1 mg/kg), which is a steroidal drug that inhibits immune and inflammatory responses, including cytokine production. CONCLUSION: The extract of Pyrostegia venusta attenuated the depressive-like and exploratory behaviors induced by lipopolysaccharide. Thus, our results supported previous claims of the usefulness of these plants in traditional therapies and suggest that these plants may be useful in the treatment of disorders that induced sickness behavior, such as flu and cold.
Subject(s)
Behavior, Animal/drug effects , Bignoniaceae/chemistry , Illness Behavior/drug effects , Lipopolysaccharides/pharmacology , Plant Extracts/pharmacology , Animals , Chromatography, High Pressure Liquid , Flowers/chemistry , Male , Mice , Motor Activity/drug effects , Plant Extracts/isolation & purificationABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: Sonchus oleraceus L. has been used to relieve headaches, general pain, hepatitis, infections, inflammation and rheumatism in Brazilian folk medicine. Nevertheless, scientific information regarding this species is scarce; there are no reports related to its possible anti-inflammatory effects. AIM OF THE STUDY: This study was aimed at evaluating the scientific basis for the traditional use of Sonchus oleraceus using in vivo inflammatory models. MATERIALS AND METHODS: Carrageenan-induced paw edema, peritonitis and febrile response induced by lipopolysaccharide tests, as well as fibrovascular tissue growth induced by s.c. cotton pellet implantation were used to investigate the anti-inflammatory activity of Sonchus oleraceus hydroethanolic extract (SoHE) in rats. RESULTS: The SoHE at test doses of 100-300 mg/kg p.o. clearly demonstrated anti-inflammatory effects by reduced paw edema induced by carragenan, inhibited leukocyte recruitment into the peritoneal cavity and reduced LPS-induced febrile response, and in the model of chronic inflammation using the cotton pellet-induced fibrovascular tissue growth in rats, the SoHE significantly inhibited the formation of granulomatous tissue. The extract administered at 300 mg/kg p.o. had a stronger anti-inflammatory effect than indomethacin (10mg/kg) or dexamethasone (1mg/kg). CONCLUSION: The hydroethanolic extract of Sonchus oleraceus markedly demonstrated anti-inflammatory action in rats, which supports previous claims of its traditional use.
