Dual Action of Beauveria bassiana (Hypocreales; Cordycipitaceae) Endophytic Stains as Biocontrol Agents against Sucking Pests and Plant Growth Biostimulants on Melon and Strawberry Field Plants.

Entomopathogenic fungi (EPF) can colonize plant tissues and serve crops not only as biopesticides but also as biostimulants that promote plant growth and trigger defense mechanisms. In this context, field trials were conducted evaluating two commercial strains of the entomopathogen Beauveria bassiana (Hypocreales: Cordycipitaceae), GHA (Botanigard) and PPRI 5339 (Velifer® ES) and a wild strain (AP0101) isolated from Achaia, Greece. The three strains were investigated in the field for their endophytic effects on melon Cucumis melo (Cucurbitales: Cucurbitaceae) and strawberry Fragaria sp. (Rosales: Rosaceae) plants and in particular for their ability to colonize plant tissues, control infestations of sucking insects Aphis gossypii (Hemiptera: Aphididae), Chaetosiphon fragaefolii (Hemiptera: Aphididae) and Frankliniella occidentalis (Thysanoptera: Thripidae), and improve plant growth parameters (plant height, number of flowers and fruits). All experimental fungal strains successfully colonized both plants. A significant decrease in the aphid and thrip populations was observed in the treated plants compared to the untreated control. As for plant growth, the number of flowers and fruits was significantly increased in plants treated with B. bassiana strains AP0101 and PPRI 5339. Our results clearly indicate that fungal endophytes can efficiently act as dual action agents demonstrating both insecticidal and growth-promoting effects.

Effect of Pyraclostrobin Application on Viral and Bacterial Diseases of Tomato.

Quinone outside inhibitors (QoI) are powerful fungicides, which have been reported, additionally to their fungicide activity, to increase plant capacity to activate cellular defense responses and to promote plant growth. In this work, the effect of the QoI class fungicide pyraclostrobin was examined against Cucumber mosaic virus (CMV), Potato virus Y (PVY) and Pseudomonas syringae pv. tomato in tomato plants following artificial inoculation of the plants with the pathogens. Under controlled environmental conditions, pyraclostrobin delayed viral and bacterial disease development, even if P. syringae pv. tomato internal population levels were not affected significantly. In contrast, under field conditions in commercial greenhouses, a reduced CMV disease incidence throughout the tomato cultivation period was recorded. Gene expression analysis indicated an effect of pyraclostrobin application on tomato MAPKs transcript levels and a possible interference with plant stress responses.