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1.
Morphologie ; 108(361): 100757, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38134867

ABSTRACT

Voice disorders are common among teachers and, in particular, anatomy teachers are exposed to a potential enemy for dysphonia, irritating chemicals, that is, formaldehyde. We seek to verify the association between: (1) teaching time, (2) type of cadaveric conservation to which the teacher is exposed and (3) hours of exposure to cadaveric preservative related to the different categories of voice disorders screening (ITDV). The sample consisted of 111 teachers who answered to 02 data collection instruments: I - Sociodemographic Data; II - ITDV. Among participating teachers there were 71 male and 40 female, with an average age of 43 years and 11 months and an average teaching time of 16 years and 5 months. Association tests between teaching time and ITDV demonstrate a significant result in the relationship between voice failure and teaching time (p<0.05). All 111 teachers use their voices in laboratory classes and use cadaveric material. From those, 107 teachers are exposed to formaldehyde as cadaveric parts' conservative solution. There was a significant association (p<0.05) between voice failure and the type of cadaveric conservative solution but non-significant relationship (p>0.05) between ITDV and the time of exposure to formaldehyde preservative. Teachers' ITDV showed vocal signs and symptoms. In particular, voice loss due to time of teaching in anatomy, and voice failure, due to exposure to formaldehyde and combinations used in anatomical parts and cadavers, were significant.


Subject(s)
Anatomy , Cadaver , Formaldehyde , Humans , Formaldehyde/adverse effects , Female , Male , Adult , Anatomy/education , Middle Aged , Voice Disorders/diagnosis , Voice Disorders/etiology , Voice Disorders/chemically induced , Occupational Diseases/diagnosis , Occupational Diseases/etiology , Occupational Diseases/epidemiology , Occupational Exposure/adverse effects , Time Factors , Fixatives/adverse effects , Faculty/statistics & numerical data
2.
Environ Sci Pollut Res Int ; 30(40): 93155-93164, 2023 Aug.
Article in English | MEDLINE | ID: mdl-37505383

ABSTRACT

Pyrolysis is an effective method to valorize plastic waste and obtain value-added fuels. This study adopted the ANN-GA (artificial neural network-genetic algorithm) coupled with a central composition factorial design to optimize the oil production from the pyrolysis of waste polyolefins (WP). The interactive effects of PE mass fraction (20-80 wt%), residence time (20-60 min), and carrier gas flow rate (0-100 mL/min) on the yields of WP pyrolysis products were investigated extensively by ANN. Moreover, the highest WP pyrolysis oil production of 78.87 wt%, optimized by GA, was obtained under 80 wt% PE, 60 min, and 0 mL/min. It was found that the different conditions of PE mass fraction, residence time, and carrier gas flow rate did not change the types of oil's main functional groups (-CH2-, -C=C-, -C=CH2, -CH3, and =C-H). The conditions affected the WP pyrolysis oil fractions significantly. The highest diesel selectivity of 91.42% was obtained under 20 wt% PE, 20 min, and 0 mL/min. Additionally, according to the interactive effects of different conditions on the productions of WP pyrolysis products, the pyrolysis pathways were proposed to understand the pyrolysis mechanism of WP better.


Subject(s)
Pyrolysis , Solid Waste , Polyenes , Plastics , Gasoline
3.
Sci Total Environ ; 859(Pt 1): 160133, 2023 Feb 10.
Article in English | MEDLINE | ID: mdl-36402333

ABSTRACT

Sanitation equity and climate actions are world concerns stated by the United Nations in the Sustainable Development Goals. A significant source of greenhouse gas emissions is inputted by human wastes, either in developing countries through wastewater treatment plants, or in the underdeveloped world, through anaerobic digestion of fecal sludge in pit latrines. For the first time, an integrated process for CO2 reduction and capture is implemented in a thermally sustainable, latrine-like device that destroys fresh human feces using smoldering combustion, the FeD-Latrine. A gas looping oxidizes combustible gases and creates favorable conditions to capture CO2 in bed. CH4 and H2 molar fractions are decreased around 90 % and 30 %, respectively. CaO used as a sorbent captures up to 8 mmol of CO2 per gram, forming a stable CaCO3. Compared to kinetic-dominant processes for CO2 capture, we obtain an efficiency of around 52 %. Our findings show that using the FeD-Latrine to replace typical pit latrines reduces 60 % of the CO2-eq emissions.


Subject(s)
Carbon Dioxide , Toilet Facilities , Humans , Carbon Dioxide/analysis , Sanitation , Sewage , Gases
4.
Rev. APS ; 24(2): 367-379, 2021-11-05.
Article in Portuguese | LILACS | ID: biblio-1359425

ABSTRACT

O trabalho buscou identificar o conhecimento do usuário e suas propostas acerca da oferta de serviços específicos à saúde do homem na Atenção Básica, tema de poucos estudos publicados. Foram entrevistados 101 homens nas 14 equipes da Estratégia Saúde da Família da zona urbana de um município do Estado de Minas Gerais. Utilizou-se a técnica de Análise Temática. Identificou-se insatisfação com o tempo de espera para o atendimento, marcação de consultas; falta de empatia dos profissionais de saúde; criticou-se a ausência de ações de promoção à saúde, divulgação de campanhas, falhas na administração pública e busca ativa para serviços em saúde do homem; a questão de gênero refletiu o contexto social do "ser homem", sendo o serviço de saúde tradicionalmente organizado na perspectiva materno-infantil, o que marginaliza o público-alvo e não contempla as dimensões da masculinidade.


This study aims to identify the user's knowledge and proposals about providing specific services towards men's health in Primary Care, the subject of few published studies. One hundred one men were interviewed in the 14 Family Health Strategy teams in an urban area of a municipality located in the state of Minas Gerais. The Thematic Analysis technique has been employed. Dissatisfaction concerning extended waiting periods for assistance, scheduling appointments, lack of empathy of health professionals, criticism about the absence of actions to promote health, dissemination campaigns, flaws in public administration, and active search for men's health services were identified; the gender issue reflected the social context of "being a man", with the health service traditionally organized from a maternal and child perspective, marginalizing the target audience and not including the masculinity dimensions.


Subject(s)
Men's Health
5.
Rev. Pesqui. (Univ. Fed. Estado Rio J., Online) ; 13: 415-420, jan.-dez. 2021. tab
Article in English, Portuguese | BDENF - Nursing, LILACS | ID: biblio-1151286

ABSTRACT

Objetivo: identificar os comportamentos agressivos e as reações à agressão entre escolares e verificar se há associação com as variáveis idade, sexo, escolaridade, situação conjugal dos pais e ao tipo de instituição escolar. Método: estudo descritivo, transversal e quantitativo. População composta por 492 crianças, a qual gerou uma amostra de 26 crianças da escola privada e 86 da escola pública, que responderam ao Questionário de Comportamentos Agressivos e Reativos entre Pares. Os dados passaram pela análise descritiva e o teste exato de Fisher. Resultados: a maioria das crianças apresentou agressividade e reações agressivas abaixo da média. E no que se refere as reações a agressividade as mais frequentes foram as respostas internalizadas. Conclusão: os dados obtidos podem nortear intervenções de combate e prevenção da agressividade escolar. Porém, ficou evidente que será necessário desenvolver estudos com designs metodológicos mais robustos


Objective: to identify aggressive behaviors and reactions to aggression among schoolchildren and to verify if there is an association with the variables age, sex, schooling, parents' marital status and type of school institution. Method: descriptive, cross - sectional and quantitative study. A population composed of 492 children, which generated a sample of 26 children from the private school and 86 from the public school, who answered the Questionnaire on Aggressive and Reactive Behaviors among Peers. Data were subjected to descriptive analysis and Fisher's exact test. Results: most children presented aggression and aggressive reactions below average. And the most frequent reactions to aggression were the internalized responses. Conclusion: the data obtained can guide interventions to combat and prevent school aggression. However, it has become evident that it will be necessary to develop studies with more robust methodological designs


Objetivo: identificar los comportamientos agresivos y las reacciones a la agresión entre escolares y verificar si hay asociación con las variables edad, sexo, escolaridad, situación conyugal de los padres y al tipo de institución escolar. Método: estudio descriptivo, transversal y cuantitativo. Población compuesta por 492 niños, la cual generó una muestra de 26 niños de la escuela privada y 86 de la escuela pública, que respondieron al Cuestionario de Comportamientos Agresivos y Reactivos entre Pares. Los datos pasaron por el análisis descriptivo y la prueba exacta de Fisher. Resultados: la mayoría de los niños presentó agresividad y reacciones agresivas por debajo de la media. Y en lo que se refiere a las reacciones a agresividad las más frecuentes fueron las respuestas internalizadas. Conclusión: los datos obtenidos pueden orientar intervenciones de combate y prevención de la agresividad escolar. Sin embargo, resulta evidente que será necesario desarrollar estudios con diseños metodológicos más robustos


Subject(s)
Humans , Male , Female , Child , Students , Health Education , Aggression/psychology , Child , Child Behavior , Child Development , Causality
6.
Rev Esc Enferm USP ; 54: e03589, 2020.
Article in Portuguese, English | MEDLINE | ID: mdl-32813787

ABSTRACT

OBJECTIVE: To evaluate the capacity of Primary Healthcare to coordinate healthcare networks. METHOD: A cross-sectional, population-based and descriptive study developed in the State of Minas Gerais through interviews with nurses working in the Family Health Strategy Sector (FHS) of the Unified Health System, using the Network Coordination Assessment Tool for Primary Healthcare. A descriptive statistical analysis was applied by implementing Fisher's exact test, Spearman's correlation coefficient and cluster analysis. Type I error was fixed at 5% for statistical significance. RESULTS: There were 49 nurses interviewed, evaluating the population and primary healthcare dimensions as excellent. Support, logistical, governance and overall assessment systems were classified as having good condition in the network coordination for Primary Healthcare. The dendograms showed that the work process has similarities between Nurses, as well as the structure between the municipalities. CONCLUSION: Primary Healthcare has the ability to coordinate networks, constituting a process in which nurses have a central role. There were similarities in the work processes between evaluated municipalities and a deficit in information and pharmaceutical assistance systems.


Subject(s)
Family Health , Nurses , Primary Health Care/organization & administration , Brazil , Cross-Sectional Studies , Delivery of Health Care , Humans
7.
Rev. Esc. Enferm. USP ; 54: e03589, 2020. tab, graf
Article in English | BDENF - Nursing, LILACS | ID: biblio-1125582

ABSTRACT

Abstract Objective: To evaluate the capacity of Primary Healthcare to coordinate healthcare networks. Method: A cross-sectional, population-based and descriptive study developed in the State of Minas Gerais through interviews with nurses working in the Family Health Strategy Sector (FHS) of the Unified Health System, using the Network Coordination Assessment Tool for Primary Healthcare. A descriptive statistical analysis was applied by implementing Fisher's exact test, Spearman's correlation coefficient and cluster analysis. Type I error was fixed at 5% for statistical significance. Results: There were 49 nurses interviewed, evaluating the population and primary healthcare dimensions as excellent. Support, logistical, governance and overall assessment systems were classified as having good condition in the network coordination for Primary Healthcare. The dendograms showed that the work process has similarities between Nurses, as well as the structure between the municipalities. Conclusion: Primary Healthcare has the ability to coordinate networks, constituting a process in which nurses have a central role. There were similarities in the work processes between evaluated municipalities and a deficit in information and pharmaceutical assistance systems.


Resumen Objetivo: Evaluar la capacidad de la Atención Primaria en Salud de coordinar las Redes de Atención Sanitaria. Método: Estudio transversal, poblacional, de naturaleza descriptiva, desarrollado en el Estado de Minas Gerais mediante entrevista con enfermeros que actúan en la Estrategia de Salud de la Familia, utilizando el Instrumento de Evaluación de la coordinación de las Redes por la Atención Primaria. Se aplicó el análisis estadístico descriptivo, la prueba Exacta de Fisher, el Coeficiente de correlación de Spearman y el análisis de conglomerados (Cluster). Se fijó error tipo I en el 5% como estadísticamente significativo. Resultados: Se entrevistaron 49 enfermeros, quienes evaluaron las dimensiones población y atención primaria en salud como excelentes. Sistemas de apoyo logístico, gobernanza y evaluación global fueron clasificados como teniendo condición buena en la coordinación de las redes por la Atención Primaria. Los dendogramas mostraron que el proceso laboral presenta semejanzas entre los Enfermeros, así como la estructura entre los municipios. Conclusión: La Atención Primaria tiene capacidad de coordinar las redes, proceso en el que el Enfermero juega un rol de centralidad. Hubo similitudes en el proceso laboral entre los municipios evaluados y un déficit en los sistemas de información y asistencia farmacéutica.


Resumo Objetivo: Avaliar a capacidade da Atenção Primária à Saúde de coordenar as Redes de Atenção à Saúde. Método: Estudo transversal, populacional, de natureza descritiva, desenvolvido no Estado de Minas Gerais por meio de entrevista com enfermeiros que atuam na Estratégia de Saúde da Família, utilizando o Instrumento de Avaliação da Coordenação das Redes pela Atenção Primária. Aplicou-se análise estatística descritiva; Teste Exato de Fisher, Coeficiente de correlação de Spearman e análise de agrupamento de Cluster. Fixou-se erro tipo I em 5% como estatisticamente significativo. Resultados: Entrevistaram-se 49 enfermeiros, que avaliaram as dimensões população e atenção primária à saúde como ótimas. Sistemas de apoio, logístico, governança e a avaliação global foram classificados como tendo condição boa na coordenação das redes pela Atenção Primária. Os dendogramas mostraram que o processo de trabalho apresenta semelhanças entre os Enfermeiros, assim como a estrutura entre os municípios. Conclusão: A Atenção Primária possui capacidade de coordenar as redes, processo no qual o Enfermeiro tem papel de centralidade. Houve semelhanças no processo de trabalho entre municípios avaliados e um déficit nos sistemas de informação e assistência farmacêutica.


Subject(s)
Humans , Male , Female , Adult , Primary Nursing , Program Evaluation , Health Services , Cross-Sectional Studies
8.
Ciênc. cuid. saúde ; 19: e46760, 20200000. tab
Article in Portuguese | LILACS-Express | LILACS, BDENF - Nursing | ID: biblio-1375085

ABSTRACT

RESUMO Objetivo: avaliar a satisfação do usuário do sexo masculino com os serviços oferecidos na atenção primária à saúde. Método: estudo descritivo, transversal, realizado em unidades da Estratégia de Saúde da Família por meio de entrevista com 104 homens, utilizando questionário social e instrumento validado. Na análise, utilizou-se o teste Exato de Fisher, considerando a significância de 5%. Resultado: o perfil predominante foi de homens brancos, heterossexuais, casados, com idade entre 50 e 59 anos, com emprego fixo, renda mensal entre um e dois salários mínimos e que não concluíram o ensino fundamental. Os maiores percentuais de satisfação foram com a cordialidade, o exame clínico e a atenção dada às queixas pelo médico/enfermeiro. O tempo em sala de espera, o acesso e o agendamento da consulta apresentaram os menores percentuais de satisfação. Usuários com ensino médio incompleto apresentaram avaliação mais positiva em relação à consulta, cordialidade do profissional e o tempo na sala de espera (p<0,05). Por sua vez, as explicações recebidas em consulta geraram mais insatisfação entre sujeitos que utilizam o serviço anualmente. Conclusão: os homens participantes do estudo estão mais insatisfeitos com a organização dos serviços de saúde do que com o atendimento dos profissionais


RESUMEN Objetivo: evaluar la satisfacción del usuario del género masculino con los servicios ofertados en la atención primaria de salud. Método: estudio descriptivo, transversal, conducido en unidades de la Estrategia de Salud de la Familia, mediante entrevistas con 104 hombres, utilizando cuestionario social e instrumento validado. En el análisis, se utilizó la prueba Exacta de Fisher, considerando la significación del 5%. Resultado: el perfil predominante consistió en hombres blancos, heterosexuales, casados, con edades entre 50 y 59 años, con empleo fijo, ingreso mensual entre uno y dos salarios mínimos y que no terminaron la escuela primaria. Los porcentajes más altos de satisfacción fueron con la cordialidad, el examen clínico y la atención prestada a las quejas por parte del médico/enfermero. El tiempo en la sala de espera, el acceso y la programación de consultas tuvieron los porcentajes más bajos de satisfacción. Los usuarios con escuela secundaria incompleta presentaron una evaluación más positiva con respecto a la consulta, la cordialidad del profesional y el tiempo en la sala de espera (p<0,05). A su vez, las explicaciones recibidas en las consultas generaron más insatisfacción entre los sujetos que usan el servicio anualmente. Conclusión: los hombres que participan en el estudio están más insatisfechos con la organización de los servicios de salud que con la atención brindada por los profesionales.


ABSTRACT Objective: To evaluate the satisfaction of male users with the services offered in primary health care. Method: descriptive cross-sectional study conducted in Family Health Strategy units through interviews with 104 men, using a social questionnaire and a validated instrument. The Fisher's exact test was used to analyze the data, considering the significance level of 5%. Result: the predominant profile was white, heterosexual, married men aged between 50 and 59 years, with fixed employment, monthly income between one and two minimum wages, and who had not completed elementary school. The highest percentages of satisfaction were found in the aspects of friendliness, clinical examination, and attention given by the doctor/nurse to the complaints presented. Length of time spent in the waiting room, access to consultations, and scheduling of consultations had the lowest percentages of satisfaction. Users with incomplete high school made a more positive assessment of the aspects consultation, friendliness of the professional, and time spent in the waiting room (p < 0.05). In turn, the explanations received in consultations generated more dissatisfaction among subjects who use the service in an annual basis. Conclusion: the men participating in the study were more dissatisfied with the organization of health services than with the care provided by professionals.

9.
Ciênc. cuid. saúde ; 19: e46760, 20200000.
Article in Portuguese | LILACS, BDENF - Nursing | ID: biblio-1117916

ABSTRACT

Objective: To evaluate the satisfaction of male users with the services offered in primary health care. Method:descriptive cross-sectional study conducted in Family Health Strategy units through interviews with 104 men, using a social questionnaire and a validated instrument. The Fisher's exact test was used to analyze the data, considering the significance level of 5%. Result:the predominant profile was white, heterosexual, married men aged between 50 and 59 years, with fixed employment, monthly income between one and two minimum wages, and who had not completed elementary school. The highest percentages of satisfaction were found in the aspects of friendliness, clinical examination, and attention given by the doctor/nurse to the complaints presented. Length of time spent in the waiting room, access to consultations, and scheduling of consultations had the lowest percentages of satisfaction. Users with incomplete high school made a more positive assessment of the aspects consultation, friendliness of the professional, andtime spent in the waiting room (p < 0.05). In turn, the explanations received in consultations generated more dissatisfaction among subjects who use the service in an annual basis. Conclusion:the men participating in the study were more dissatisfied withthe organization of health services than with the care provided by professionals.


Objetivo: avaliar a satisfação do usuário do sexo masculino com os serviços oferecidos na atenção primária à saúde. Método: estudo descritivo, transversal, realizado em unidades da Estratégia de Saúde da Família por meio de entrevista com 104 homens, utilizando questionário social e instrumento validado. Na análise, utilizou-se o teste Exato de Fisher, considerando a significância de 5%. Resultado: o perfil predominante foi de homens brancos, heterossexuais, casados, com idade entre 50 e 59 anos, com emprego fixo, renda mensal entre um e dois salários mínimos e que não concluíram o ensino fundamental. Os maiores percentuais de satisfação foram com a cordialidade, o exame clínico e a atenção dada às queixas pelo médico/enfermeiro. O tempo em sala de espera, o acesso e o agendamento da consulta apresentaram os menores percentuais de satisfação. Usuários com ensino médio incompleto apresentaram avaliação mais positiva em relação à consulta, cordialidade do profissional e o tempo na sala de espera (p<0,05). Por sua vez, as explicações recebidas em consulta geraram mais insatisfação entre sujeitos que utilizam o serviço anualmente. Conclusão: os homens participantes do estudo estão mais insatisfeitos com a organização dos serviços de saúde do que com o atendimento dos profissionais.


Subject(s)
Humans , Male , Middle Aged , Primary Health Care , Patient Satisfaction , Health Services , Men , Referral and Consultation , Unified Health System , Family , Health Personnel , Disease Prevention , Men's Health , Hospitalization
10.
Rev. enferm. UFPE on line ; 11(supl.12): 5339-5353, dez.2017. ilus, tab, graf
Article in Portuguese | BDENF - Nursing | ID: biblio-1032364

ABSTRACT

Objetivo: avaliar as competências dos ACS no desenvolvimento das atribuições esperadas e definidas peloMinistério da Saúde nas ações de pré-natal de risco habitual, desenvolvidas no âmbito da ESF, em relação àprevenção da infecção pelo zika vírus na gestação. Método: estudo quantitativo, descritivo, transversal, comcoleta de dados em 14 equipes da Estratégia de Saúde da Família, mediante um questionário com três partes:A (enfrentamento ao Aedes aegypti); B (pré-natal de baixo risco) e C (medidas de prevenção pessoal contra oZika vírus), analisadas por agrupamentos e pelo Teste Exato de Fisher. Resultados: melhor desempenho foiobservado em B, seguido de A. As maiores dificuldades estavam relacionadas com a atividade C. Conclusão:há um panorama favorável de atuação desses profissionais, embora com dificuldades quanto à alimentaçãodos sistemas de informação, à baixa participação comunitária e à pouca integração com o trabalho dasequipes.


Subject(s)
Male , Female , Humans , Adult , Middle Aged , Aged , Aedes , Community Health Workers , Clinical Competence , Prenatal Care , National Health Strategies , Pregnant Women , Zika Virus Infection/prevention & control , Zika Virus , Epidemiology, Descriptive , Cross-Sectional Studies , Surveys and Questionnaires
11.
Hig. aliment ; 30(262/263): 136-141, 30/12/2016.
Article in Portuguese | LILACS | ID: biblio-832053

ABSTRACT

O objetivo deste trabalho foi desenvolver e caracterizar iogurte adicionado de geleiada de casca de jabuticaba e de Lactobacilos acidophilus LA-3 e analisar suas características microbiológicas e físico-químicas. Inicialmente, preparou-se geleiada com o resíduo do processamento de jabuticaba. Em seguida, o iogurte foi processado, adicionado de 5% de geleiada e dividido em dois tratamentos: iogurte adicionado de L. acidophilus LA-3 e controle, sem adição da cultura. Realizou-se análises de pH, acidez titulável, sólidos solúveis, coliformes a 30°C e a 45°C e de fungos filamentosos e leveduras nas amostras de geleiada. As amostras de iogurte foram submetidas às análises de pH, acidez titulável, extrato seco total, umidade, gordura, cinzas, proteína, além da contagem de bactérias láticas, fungos filamentosos e leveduras e coliformes a 30°C e a 45°C. Verificou-se resultados médios de 2,38 para pH, 1,166 para acidez (% ácido cítrico) e 67,33 °Brix para sólidos solúveis nas amostras de geleiadas. Por outro lado, as amostras de iogurte do tratamento controle diferenciaram daquelas adicionadas de cultura probiótica (p<0,05) em relação a pH, acidez, extrato seco e umidade. Os teores de cinzas e gordura não diferiram (p>0,05) entre os tratamentos ao longo do tempo. Constatou-se <3,0 NMP/g de coliformes a 30°C e a 45°C em ambos os tratamentos e <1,0x101 UFC/g estimado para fungos filamentosos e leveduras para geleiada e para iogurte. As contagens de L. acidophilus LA-3 e de L. bulgaricus no iogurte foram acima de 108 UFC/g logo após a produção, enquanto após 30 dias a 5°C observou- -se contagens acima de 107 UFC/g. Streptococus thermophilus manteve- -se acima 109 UFC/g durante a estocagem. Portanto, o iogurte contendo geleiada de casca de jabuticaba pode ser utilizado como substrato potencial para L. acidophilus LA-3 e para as culturas láticas uma vez que estas se mantiveram com elevada viabilidade celular durante a vida de prateleira. O produto elaborado pode ser considerado de dupla funcionalidade por unir as características das culturas a da casca de jabuticaba.


Subject(s)
Humans , Yogurt/analysis , Probiotics , Functional Food , Fruit Jam , Food Analysis , Lactobacillus acidophilus
12.
Int Immunopharmacol ; 7(6): 824-35, 2007 Jun.
Article in English | MEDLINE | ID: mdl-17466916

ABSTRACT

In this study, we tested the potential use of a lectin from Lonchocarpus sericeus seeds (LSL), to control neutrophil migration and inflammatory hypernociception (decrease of nociceptive threshold). Pretreatment of the animals intravenously (15 min before) with LSL inhibited neutrophil migration to the peritoneal cavity in a dose-dependent fashion confirmed by an inhibition of rolling and adhesion of leukocytes by intravital microscopy. We also tested the ability of the pretreatment with LSL to inhibit neutrophil migration on immunised mice, and it was observed that a strong inhibition of neutrophil migration induced by ovoalbumin in immunized mice. Another set of experiments showed that pretreatment of the animals with LSL, inhibited the mechanical hypernociception in mice induced by the i.pl. injection of OVA in immunized mice and of carrageenan in naïve mice, but not that induced by prostaglandin E(2) (PGE(2)) or formalin. This anti-nociceptive effect correlated with an effective blockade of neutrophil influx, as assessed by the hind paw tissue myeloperoxidase levels. In addition, we measured cytokines (TNF-alpha and IL-1beta) and chemokines (MIP-1alpha [CCL3] and KC [CXCL1]) from the peritoneal exudates and i.pl. tissue. Animals treated with LSL showed inhibition of cytokines and chemokines release in a dose-dependent manner. In conclusion, we demonstrated that the inhibitory effects of LSL on neutrophil migration and mechanical inflammatory hypernocicepetion are associated with the inhibition of the production of cytokines and chemokines.


Subject(s)
Analgesics/pharmacology , Neutrophils/drug effects , Pain/drug therapy , Plant Lectins/pharmacology , Animals , Anti-Inflammatory Agents/pharmacology , Carrageenan , Cell Adhesion/drug effects , Cell Movement/drug effects , Cytokines/immunology , Dinoprostone , Fabaceae/chemistry , Formaldehyde , Immunoglobulin G/immunology , Male , Mice , Mice, Inbred BALB C , Neutrophils/immunology , Ovalbumin/immunology , Pain/chemically induced , Pain/immunology , Peroxidase/metabolism
13.
Int J Biochem Cell Biol ; 37(9): 1805-14, 2005 Sep.
Article in English | MEDLINE | ID: mdl-15914076

ABSTRACT

Arum maculatum agglutinin (AMA) is a monocot lectin isolated from tubers of Arum maculatum L. (Araceae) which exhibits different specificity towards oligo-mannosidic-type and N-acetyllactosaminic-type glycans. We have investigated the effect of this lectin on the cells of the immune system. Models of neutrophil migration in vivo, neutrophil chemotaxis in vitro and macrophage cultures were used to study the lectin inflammatory activity. When administered into rat peritoneal cavities, AMA (80, 200 and 500 microg/mL/cavity) induced significant and dose-dependent neutrophil migration. This effect was inhibited by incubation with alpha-methyl-d-mannoside. A 83% depletion in the number of resident cells following peritoneal lavage did not reduce the AMA-induced neutrophil migration, as compared to sham animals (not washed). However, pre-treatment with 3% thioglycolate which increases the peritoneal macrophage population by 236%, enhanced the neutrophil migration induced by AMA (200 microg/mL/cavity) (119%, p < 0.05). Reduction of peritoneal mast cell population by chronic treatment of cavities with compound 48/80 did not modify AMA-induced neutrophil migration. The neutrophil chemotaxy assay in vitro shows that the lectin (300 microg/mL) induces neutrophil chemotaxy (368% p < 0.05) compared to RPMI. Finally, injection into peritoneal cavities of supernatants from macrophage cultures obtained after stimulation with AMA (300 microg/mL) enhanced neutrophil migration (110% p < 0.05). Summarizing, our data suggest that A. maculatum agglutinin presents pro-inflammatory activity, inducing neutrophil migration by two ways, one which is independent on resident cells and another one dependent on the presence of these cells.


Subject(s)
Arum/chemistry , Chemotaxis, Leukocyte/drug effects , Macrophages, Peritoneal/immunology , Neutrophils/immunology , Plant Lectins/pharmacology , Animals , Cell Count , Cell Movement/drug effects , Cells, Cultured , Female , Male , Mast Cells/drug effects , Peritoneal Cavity/cytology , Rats , Rats, Wistar , Thioglycolates/pharmacology
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