ABSTRACT
Two important parameters for determination of deleterious effects of cellular processing on hematopoietic progenitor cells are cell viability and concentration. The Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins Hospital evaluated the Beckman Coulter Vi-Cell automated instrument for the measurement of these two parameters. Using 33 thawed hematopoietic progenitor cell samples, automated Vi-Cell viability results were compared to those obtained using the standard trypan blue manual method. In addition, cell concentrations from these samples were compared with results from the Model Z2 Coulter Counter. Chinese Hamster Ovary cells were used for the evaluation of Vi-Cell linearity at the Beckman Coulter Cellular Analysis Development Center. Significant correlation was obtained when the two methods were compared for both cell concentration and percentage viability (P < .0001). The results of the linearity study indicated that the Vi-Cell is linear from approximately 5 x 10(4) to greater than 1 x 10(7) cells/mL. The Vi-Cell uses sample volumes as low as 0.5 mL; cell diameters may be 2 to 70 microns. The Vi-Cell automated instrument offers many significant advantages for cell analyses in today's busy laboratory environment.
Subject(s)
Cell Count/instrumentation , Cell Survival , Evaluation Studies as Topic , Hematopoietic Stem Cells , Animals , Autoanalysis/instrumentation , Autoanalysis/methods , COS Cells , Cell Count/methods , Cricetinae , Cryopreservation , Humans , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
The majority of the nursing home residents in this study demonstrated a cordial, nonconflictive bond with their roommates. More positive bonds were associated with a greater number of positive emotions and greater satisfaction with the nursing home. Overall, results suggest nursing interventions designed to enhance roommate bondedness could promote psychological health and quality of life among a group of frail older adults.
Subject(s)
Aged/psychology , Emotions , Inpatients/psychology , Interpersonal Relations , Nursing Homes , Object Attachment , Aged, 80 and over , Female , Health Status , Humans , Male , Middle Aged , Nursing Methodology Research , Patients' Rooms , Peer Group , Surveys and QuestionnairesABSTRACT
Interviews were conducted with 332 intellectually intact residents in 54 nursing homes to study their patterns of interpersonal relationships with staff, other residents, and family and friends outside the home. Results indicate the importance of having a close friend for residents' participation in activities, life satisfaction, and nursing home satisfaction.
