ABSTRACT
Stem cells in different regions of the nervous system give rise to different types of mature cells. This diversity is assumed to arise in response to local environmental differences, but the contribution of cell-intrinsic differences between stem cells has been unclear. At embryonic day (E)14, neural crest stem cells (NCSCs) undergo primarily neurogenesis in the gut but gliogenesis in nerves. Yet gliogenic and neurogenic factors are expressed in both locations. NCSCs isolated by flow-cytometry from E14 sciatic nerve and gut exhibited heritable, cell-intrinsic differences in their responsiveness to lineage determination factors. Gut NCSCs were more responsive to neurogenic factors, while sciatic nerve NCSCs were more responsive to gliogenic factors. Upon transplantation of uncultured NCSCs into developing peripheral nerves in vivo, sciatic nerve NCSCs gave rise only to glia, while gut NCSCs gave rise primarily to neurons. Thus, cell fate in the nerve was stem cell determined.
Subject(s)
Cell Differentiation/physiology , Cell Lineage/physiology , Enteric Nervous System/embryology , Neural Crest/embryology , Neuroglia/metabolism , Neurons/metabolism , Sciatic Nerve/embryology , Stem Cells/metabolism , Animals , Bone Morphogenetic Protein 4 , Bone Morphogenetic Proteins/metabolism , Bone Morphogenetic Proteins/pharmacology , Cell Communication/drug effects , Cell Communication/genetics , Cell Differentiation/drug effects , Cell Division/drug effects , Cell Division/genetics , Cell Lineage/drug effects , Cell Movement/drug effects , Cell Movement/genetics , Cells, Cultured , Clone Cells/drug effects , Clone Cells/metabolism , Enteric Nervous System/cytology , Enteric Nervous System/metabolism , Female , Fetus , Gene Expression Regulation, Developmental/drug effects , Gene Expression Regulation, Developmental/genetics , Intracellular Signaling Peptides and Proteins , Membrane Proteins/metabolism , Membrane Proteins/pharmacology , Nerve Growth Factors/metabolism , Neural Crest/cytology , Neural Crest/metabolism , Neuregulin-1/metabolism , Neuregulin-1/pharmacology , Neuroglia/cytology , Neuroglia/drug effects , Neurons/cytology , Neurons/drug effects , Pregnancy , Rats , Rats, Sprague-Dawley , Receptor, Nerve Growth Factor/drug effects , Receptor, Nerve Growth Factor/genetics , Receptor, Nerve Growth Factor/metabolism , Sciatic Nerve/cytology , Sciatic Nerve/metabolism , Stem Cells/cytology , Stem Cells/drug effectsABSTRACT
We found neural crest stem cells (NCSCs) in the adult gut. Postnatal gut NCSCs were isolated by flow-cytometry and compared to fetal gut NCSCs. They self-renewed extensively in culture but less than fetal gut NCSCs. Postnatal gut NCSCs made neurons that expressed a variety of neurotransmitters but lost the ability to make certain subtypes of neurons that are generated during fetal development. Postnatal gut NCSCs also differed in their responsiveness to lineage determination factors, affecting cell fate determination in vivo and possibly explaining their reduced neuronal subtype potential. These perinatal changes in gut NCSCs parallel perinatal changes in hematopoietic stem cells, suggesting that stem cells in different tissues undergo similar developmental transitions. The persistence of NCSCs in the adult PNS opens up new possibilities for regeneration after injury or disease.
