ABSTRACT
The study of the cell cycle of a yeast strain made it possible to define two parameters: T, the time elapsing between the appearance of two consecutive buds on a mother cell, and theta, the time elapsing between the appearance of a bud and the beginning of the first mitotic cycle. The influence of these two parameters on the growth rate of the strain is studied.
Subject(s)
Models, Biological , Saccharomyces cerevisiae/growth & development , Cell Cycle , Culture Media , MitosisABSTRACT
A study of the composition of cell walls extracted from a wild strain and three mutant strains "smooth colony" of Saccharomyces cerevisiae had been done. It points out differences in the mannose/glucose ratio and in the concentration of some other components of the cell wall in the mutant strains. The immunological study of the cell wall shows that "smooth colonies" mutants and wild strains differs in their mannoproteins.
Subject(s)
Genes , Saccharomyces cerevisiae/ultrastructure , Cell Wall/analysis , Cell Wall/immunology , Epitopes , Glucose/analysis , Mannose/analysis , Mutation , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/immunologyABSTRACT
Some fractions of the Saccharomyces cerevisiae cell wall have been prepared by the action of Helix pomatia juice on intact cells. Immunosera were obtained by injecting rabbits with these fractions. Immunofluorescence reactions, obtained with these sera, show that some fractions of mannopeptides when extracted from a "smooth-colony" mutant strain, have lost antigenic determinants.
Subject(s)
Mutation , Saccharomyces cerevisiae/immunology , Cell Wall/immunology , Epitopes , Fluorescent Antibody Technique , Fungal Proteins/immunology , Glycopeptides/immunology , Saccharomyces cerevisiae/growth & development , Subcellular FractionsABSTRACT
The mutation of one of the genes of the series PLi in Saccharomyces cerevisiae Hansen brings about a reduction in the size of the cells. Besides, the mutation of some genes (PLi 5 and PLi 7) induces a change in the scanning microscopy appearance of the cell wall.