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1.
Ann Oncol ; 17(1): 60-4, 2006 Jan.
Article in English | MEDLINE | ID: mdl-16282247

ABSTRACT

BACKGROUND: The involvement of thyroid hormones in the development and differentiation of normal breast tissue has been established. However, the association between breast cancer and these hormones is controversial. Therefore, the objective of the present study was to determine the protein expression pattern of thyroid hormone receptors in different human breast pathologies and to evaluate their possible relationship with cellular proliferation. PATIENTS AND METHODS: The presence of thyroid hormone receptors was evaluated by immunohistochemistry and western blot analysis in 84 breast samples that included 12 cases of benign proliferative diseases, 20 carcinomas in situ and 52 infiltrative carcinomas. RESULTS: TR-alpha was detected in the nuclei of epithelial cells from normal breast ducts and acini, while in any pathological type this receptor was located in the cytoplasm. However, TR-beta presented a nuclear location in benign proliferative diseases and carcinomas in situ and a cytoplasmatic location in normal breast and infiltrative carcinomas. The highest proliferation index was observed in carcinomas in situ, although in infiltrative carcinomas an inverse correlation between this index and the TR-alpha expression was encountered. CONCLUSIONS: The results of this study reveal substantial changes in the expression profile of thyroid hormone receptors suggesting a possible deregulation that could trigger breast cancer development.


Subject(s)
Breast Diseases/metabolism , Breast Neoplasms/metabolism , Cell Proliferation , Receptors, Thyroid Hormone/metabolism , Blotting, Western , Breast/metabolism , Breast Diseases/pathology , Breast Neoplasms/pathology , Carcinoma in Situ/metabolism , Carcinoma in Situ/pathology , Carcinoma, Ductal, Breast/metabolism , Carcinoma, Ductal, Breast/pathology , Carcinoma, Lobular/metabolism , Carcinoma, Lobular/pathology , Disease Progression , Female , Fibroadenoma/metabolism , Fibroadenoma/pathology , Humans , Immunohistochemistry
2.
J Anat ; 194 ( Pt 1): 109-18, 1999 Jan.
Article in English | MEDLINE | ID: mdl-10227672

ABSTRACT

A histochemical, light and electron microscopy study of the hatching gland cells (HGCs) in incubated 50-d-old trout embryos is reported. The distribution of carbohydrate residues in the glycoconjugates of these cells was studied by means of a battery of 13 different lectins conjugated with horseradish peroxidase (PNA, ConA, LCA, WGA, SBA, UEA-I, HPA, DBA) or digoxigenin (DSA, MAA, AAA, SNA, GNA). Identification of N- and O-linked oligosaccharides in HGCs was performed by application of both chemical and enzymatic treatments. Present results suggest that HGCs are seromucous cells which store both high choriolytic enzyme (HCE) and low choriolytic enzyme (LCE), and that their cytoplasmic granules, endoplasmic reticulum and Golgi complex contain additional sialic acid-rich glycoproteins. The negative charge of these glycoproteins might be responsible for the rapid expansion of mucin to form a highly hydrated gel, which would facilite the action of these enzymes in programmed cell death and might play a major role during the morphogenic events.


Subject(s)
Oligosaccharides/analysis , Trout/embryology , Animals , Cytoplasmic Granules/chemistry , Endoplasmic Reticulum, Rough/chemistry , Enzyme Precursors/analysis , Golgi Apparatus/chemistry , Histocytochemistry/methods , Lectins/metabolism , Microscopy, Electron
3.
J Anat ; 189 ( Pt 3): 609-21, 1996 Dec.
Article in English | MEDLINE | ID: mdl-8982837

ABSTRACT

A histochemical study of the branchial area of brown trout embryos from 35 to 71 d of incubation is reported. A battery of 6 different horseradish peroxidase-labelled lectins, the PAS reaction and Alcian blue staining were used to study the distribution of carbohydrate residues in glycoconjugates along the pharyngeal and branchial epithelia. Con A and WGA reacted at every site of the branchial region thus showing the ubiquitous presence of alpha-D-mannose and N-acetyl-D-glucosamine. WGA, DBA and SBA were good markers for the hatching gland cells (HGCs) and mucous cells. Other lectins, such as PNA and UEA I, reacted only for a short time at some sites during the considered period of incubation. From 35 d until posthatching stages, a manifest strong reaction was noted both in the dorsal epithelium of branchial arches and the HGCs as shown by SBA reactivity. This may be significant with regard to the controversial origin of HGCs, which is thought to be endodermal.


Subject(s)
Branchial Region/chemistry , Glycoconjugates/analysis , Lectins/metabolism , Plant Lectins , Soybean Proteins , Trout/embryology , Acetylglucosamine/analysis , Animals , Binding Sites , Concanavalin A , Epithelium/chemistry , Histocytochemistry , Mannose/analysis , Peanut Agglutinin , Trout/metabolism , Wheat Germ Agglutinins
4.
Anat Histol Embryol ; 25(4): 301-9, 1996 Dec.
Article in English | MEDLINE | ID: mdl-9011108

ABSTRACT

HGCs were found in the head epidermis, yolk sac and pharynx epithelium of trout embryos. These cells usually appear in clusters, closely related positionally to neighbouring cells. The differentiation and specialization of HGCs seem to be mainly dependent on cell-cell interactions, which provides, in part, the positional information necessary for the cells to differentiate and localize in the appropriate place. The final secretory process is the result of a sequence of events by which the maturation of enzymatic granules occurs. The electron-density of the granules varies according to the proximity of the secretory stage. Exocytosis of the secretory granules were observed. After secretion, each HGC undergoes cellular death by apoptosis (programmed cell death).


Subject(s)
Embryo, Nonmammalian/cytology , Epidermal Cells , Pharynx/cytology , Trout/embryology , Yolk Sac/cytology , Animals , Apoptosis , Cell Communication/physiology , Cell Differentiation/physiology , Cytoplasmic Granules/ultrastructure , Embryo, Nonmammalian/physiology , Embryo, Nonmammalian/ultrastructure , Epidermis/embryology , Epidermis/physiology , Epithelial Cells , Epithelium/physiology , Epithelium/ultrastructure , Exocytosis/physiology , Female , Histocytochemistry , Male , Microscopy, Electron/veterinary , Microscopy, Electron, Scanning/veterinary , Pharynx/embryology , Pharynx/physiology , Trout/physiology , Yolk Sac/embryology , Yolk Sac/physiology
5.
J Morphol ; 229(2): 201-217, 1996 Aug.
Article in English | MEDLINE | ID: mdl-29852581
6.
Anat Histol Embryol ; 24(4): 217-21, 1995 Dec.
Article in Spanish | MEDLINE | ID: mdl-8592972

ABSTRACT

The teratogenic effect of alcohol on chick embryos has been confirmed by many investigators. However, how this occurs is unknown. The aim of this study was to establish a teratogenic pattern of alcohol effects, on the first stages of development in avians. Fertilized eggs were infused through the air space of the shell on day 0, with ethanol in concentrations of 20%, 40% and 60%. The control group was infused with 0.1 ml of NaCl at 0.9%. At a second stage, the eggs were treated on the 4th day of incubation, using the same method. In both groups the eggs were removed on the 11th day of incubation. The teratological manifestations that appeared more frequently were evisceration, haemorrhagic embryos, oedema, cranial deformities, lack of eyes, and umbilical hernia, showing every embryo a clear reduction in size and body weight.


Subject(s)
Abnormalities, Drug-Induced , Chick Embryo/drug effects , Ethanol/toxicity , Animals , Chick Embryo/growth & development
7.
Anat Histol Embryol ; 24(4): 257-63, 1995 Dec.
Article in English | MEDLINE | ID: mdl-8592979

ABSTRACT

The development of the cephalic region of rainbow trout in the 24th, 30th and 36th stages, corresponding to the table of development by Vernier (1969), was studied using light microscopy and SEM. The 24th stage shows a voluminous cephalic region as a principal feature. At this time, most components of this region have started their differentiation. The 30th stage can be considered as an intermediary phase in the embryonic formation, and it is characterized by the high degree of differentiation between the nervous system and the sense organs. In the 36th stage, the basic final shape of the embryo has been reached. The results of this study show that the differentiation of gill lamellae and the opening of the mouth takes place later than that moment described by other authors. This suggests that development between 30th and 36th stages is a critical survival period for rainbow trout, in which respiratory and digestive functions start.


Subject(s)
Head/embryology , Oncorhynchus mykiss/embryology , Animals , Brain/embryology , Brain/ultrastructure , Gills/embryology , Gills/ultrastructure , Microscopy, Electron, Scanning/veterinary
8.
Theriogenology ; 41(4): 907-14, 1994.
Article in English | MEDLINE | ID: mdl-16727444

ABSTRACT

A superovulatory treatment for mice based on FSH administration was compared with a standard one based on PMSG. Our aim was to determine if a mean number of embryos recovered per donor could be increased and if in vitro or in vivo viability was affected by the hormonal treatment used. Thus, female Swiss mice were subjected to 2 superovulatory treatments, and the 1-cell and 2-cell stage embryos were cultured in 2 different media to the blastocyst stage or were transferred to pseudopregnant recipients. The data show that despite a lower mating percentage (52% with FSH vs 66% with PMSG), the FSH-treated mice provided twice the number of total embryos (53.4 vs 24.5) with a similar percentage of morphologically normal embryos (74% for FSH vs 69% for PMSG). We also found that in vitro culture results can be influenced by the source of gonadotropins depending on the culture medium used. A culture medium such as CZB which prevents the 2-cell block, provided the same developmental rates regardless of hormonal treatment used. However, with M-16 medium, which does not prevent this blockage, only 39% of the 2-cell FSH-derived embryos and 49% of the PMSG-derived 2-cell embryos developed into blastocysts (P<0.05). FSH-derived embryos resulted in a higher percentage of pregnant recipients (73 vs 56%) than PMSG-derived embryos, but the number of alive fetuses and the number of implantations per pregnant recipient was affected only by the kind of culture system used before transfer. The results show that FSH can provide very good superovulatory response in mice, thus reducing the number of donors needed for a given experiment and providing embryos of at least the same quality as those derived from the standard PMSG treatment.

9.
Anat Histol Embryol ; 18(4): 297-304, 1989 Dec.
Article in Spanish | MEDLINE | ID: mdl-2624321

ABSTRACT

In this study we examined some histologic and histochemical characteristics of the embryonic sheep dental epithelium in early odontogenesis. During the first trimester of development, a short-lived dental lamina was observed. Apparently in the sheep, the interactions between epithelial and ectomesenchymal cells required for tooth normal morphogenesis are altered.


Subject(s)
Embryonic and Fetal Development , Odontogenesis , Sheep/embryology , Tooth Germ/anatomy & histology , Animals
10.
J Morphol ; 189(2): 121-9, 1986 Aug.
Article in English | MEDLINE | ID: mdl-3746915

ABSTRACT

In this study we examined the possible inductive role of the dental papilla from polyphyodont lizard tooth germs. Flank skin sheets of quail ectoderm enzymatically separated from dermal tissue were recombined with lizard tooth papillae and placed on semisolid medium and cultured for 2 days. Subsequently, the recombinants were removed and placed on the chorioallantoic membrane of chick hosts and incubated for 6 days. After this period of 8 days in explant, control tissues differentiated according to their own phenotypes. Lizard dental papilla alone differentiated as fibroblasts. Quail flank skin ectoderm differentiated into epithelial sheets. Intact lizard tooth buds developed into teeth with dentine and incipient enamel. In the best experimental recombinants, advanced and relatively well-constructed teeth were observed, with clear indications of hard tissue deposition in association with quail epithelium. The results show that mesenchyme of the adult lizard dental papilla and embryonic quail ectoderm of heterotopic origin are capable of carrying out the complex sequence of morphogenetic interactions involved in normal odontogenesis.


Subject(s)
Amelogenesis , Coturnix/embryology , Ectoderm/physiology , Lizards/embryology , Odontogenesis , Quail/embryology , Animals , Cells, Cultured , Ectoderm/cytology , Embryo, Nonmammalian/physiology , Epithelial Cells , Female , Tooth Germ/cytology
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