Increased expression of interleukin-1beta and its novel splice variant in canine hearts with volume overload.

Volume overload frequently caused in dogs by chronic degenerative valvular disease (CDVD), eventually leads to cardiac failure. Experimental and clinical evidences demonstrate that increased interleukin-1beta serum level in patients with heart insufficiency correlates with the severity of failure irrespective of its etiology. Very little is known about the IL-1beta expression in failing vs. non-failing myocardium. IL-1beta transcript level was determined in the CDVD dogs (n=17) and control animals (n=9) without cardiac insufficiency by real-time PCR. IL-1beta transcript level in failing hearts was higher than in the control. In both groups the highest IL-1beta level was detected in the left ventricles. Although IL-1beta is a major pro-inflammatory cytokine most of the CDVD dogs displayed no inflammatory infiltrates into the myocardium. Massive fibrosis was observed in the control group, unlike the failing hearts, in which cardiomyocyte hypertrophy and atrophy dominated. The alternative IL-1beta transcript identified here (IL-1betasv1) was significantly elevated in the failing myocardium compared with the control group. Increased IL-1beta expression seems to be associated with mechanical heart overload. Its endogenous origin, and certain histopathological findings attributed to IL-1beta indicate its importance in cardiac hypertrophy and failure. The lack of some typical IL-1beta actions, i.e. inflammatory, pyrogenic and fibrotic, may suggest a different role of this cytokine in myocardium. It appears that the canine IL-1beta gene can be transcribed in two ways in heart tissue, with the IL-1betasv1 form present mainly in failing hearts.

[Magnesium in skin allergy]. / Wplyw magnezu na reakcje alergiczne skóry.

Magnesium is involved in many biological processes within the body. Magnesium deficiency causes many disorders, including impairment of immunity. This review summarizes present knowledge on the relationship between magnesium and skin allergy reactions. Special focus is on allergy types I and IV. At present the best knowledge is on allergy I. Magnesium deficiency in experimental animals, mainly rats, leads to characteristic hyperemia, an increase in IgE, neutrophilia and eosinophilia, an increase in the level of proinflammatory cytokines, mastocyte degranulation, histaminemia, and splenomegaly. These symptoms observed in hypomagnesemic rats are similar to those in atopic patients. Data on the relationship between magnesium and other types of allergy are scarce. Clinical observations show the beneficial effect of topical and oral administration of magnesium salts in patients with skin allergy. All the presented data point to an important role of magnesium in allergy reactions. Other studies are needed to better understand the mechanism of magnesium's action. Well-controlled clinical protocols should also be conducted to assess the efficiency of magnesium supplementation in patients with skin allergy.