Endocrine secretion of prostaglandin F2alpha in cyclic gilts is decreased by intrauterine administration of exogenous oxytocin.

When administered systemically, oxytocin (OT) stimulates secretion of uterine prostaglandin F2alpha (PGF2alpha) in swine, but the role of endometrially-derived OT in control of PGF2alpha release is not clear. This study determined the effect of exogenous OT, administered into the uterine lumen of intact cyclic gilts, on PGF2alpha secretion during late diestrus. Intrauterine infusion of 40USP units OT (in 30 ml 0.9% saline) was performed for 30 min (1 ml/min) into each uterine horn between 7:00 and 9:00 h on days 10, 12, 14 and 16 after estrus. Beginning 20 min before infusion, samples of jugular venous blood were drawn at 5-10-min intervals for 140 min for quantification of 13,14-dihydro-15-keto-PGF2alpha (PGFM), the major stable metabolite of PGF2alpha. Progesterone was analyzed in samples collected 0, 60 and 120 min after initiation of OT infusion. Treatment with OT did not alter plasma concentrations of PGFM on days 10 or 12 but decreased (P<0.001) PGFM concentrations for 40 min after onset of infusion on day 16. Concentrations of PGFM also were reduced in the pre-treatment samples on day 14 (P=0.05) and day 16 (P<0.001) in OT-infused gilts. Plasma progesterone declined (P<0.01) between days 10 and 16 in control-infused gilts but did not decline until after day 14 (P<0.001) in gilts infused with OT. These results indicate that when OT is administered into the uterine lumen of pigs during late diestrus, it has an anti-luteolytic effect to reduce endocrine secretion of PGF2alpha and delay the decline in progesterone that occurs during luteolysis.

Morphometric analysis of the uterine endometrium of swine on days 12 and 16 postestrus.

The uterine endometrium of swine is comprised of luminal epithelial, glandular epithelial, and stromal cells that secrete the luteolysin, prostaglandin F(2alpha) (PGF(2alpha)), during late diestrus. However, which of these cells contribute the most to luteolytic PGF(2alpha) secretion is unknown because the cellular composition of the endometrium has not been quantified. Therefore, this study quantified the cellular composition of the endometrium on days 12 and 16 postestrus by histologic and morphometric analyses. On day 12, the endometrium consisted predominantly of stromal cells (47% of total cell quantity) and glandular epithelial cells (37%), whereas luminal epithelial cells represented only 16% of the total of the three cell types. The number of glandular epithelial cells tended to increase (P < 0.10) between days 12 and 16, such that they comprised 45% of the endometrium by day 16, while the number of stromal and luminal cells did not change and accounted for 45% and 10% of the cells, respectively. Luminal epithelial cells had a 58% greater cross-sectional area (P < 0.001) than glandular epithelial cells, whereas glandular epithelial cells had a 22% greater area (P < 0.001) than stromal cells. Glandular epithelial cells decreased (P < 0.001) in cross-sectional area between days 12 and 16, whereas the area of luminal epithelial and stromal cells remained unchanged. These results indicate that the porcine endometrium is comprised predominantly of stromal and glandular epithelial cells that are likely to contribute substantially to endometrial PGF(2alpha) secretion during luteolysis. The contribution of glandular epithelium to luteolytic PGF(2alpha) secretion probably increases during diestrus as the number of these cells increases.