Comparative efficacy of tannin-free grain sorghum varieties for the control of necrotic enteritis caused by Clostridium perfringens in broiler chickens.

A 28-day battery cage study was conducted to test the efficacy of tannin-free grain sorghum varieties fed to Cobb 500 male broiler chickens (n = 512) and challenged with Eimeria maxima (EM) and Clostridium perfringens (CP). Birds were fed 1 of 8 treatments (corn, red/bronze, white/tan, or U.S. No. 2 sorghum) and were grouped by challenge method (challenged with EM/CP or unchallenged). On d 14, birds in the challenge group were orally inoculated with ∼5,000 oocysts of EM, and on d 19, 20, and 21, birds were given a broth culture of CP with ∼108 CFU/mL once daily. On d 21, three birds were scored for the degree/presence of necrotic enteritis (NE) lesions. Birds and feed were group weighed (d 0, 14, 21, and 28) to calculate average feed intake (FI), body weight gain (BWG), and adjusted feed conversion ratio (AdjFCR). Intestinal integrity was assessed through histological analysis of intestinal tissues, and change in transcriptome was determined using mRNA-sequencing on intestinal mucosa. Relative concentrations of secondary metabolites in grain sorghum were determined by LC-MS/MS analysis. Data were analyzed as a 2-way ANOVA with factors of treatment, challenge and their interaction. Regardless of challenge from 14 to 21 d, birds on the corn, white/tan, and U.S. No. 2 treatments were more efficient than those fed red/bronze treatment (P = 0.0026). From 14 to 28 d, BWG was significantly higher for the white/tan treatment (P = 0.024) compared to the red/bronze treatment. At 21 d, a significant interaction was observed for lesion score (P = 0.0001) in which, challenged birds fed red/bronze and white/tan treatments had reduced intestinal lesions compared to U.S. No. 2 and corn treatments. No differences among treatments were observed in jejunum morphology, but differential expression analysis showed an upregulation in defense response to bacteria and biotic stress in the challenged red/bronze treatment compared to the challenged corn. This study demonstrated improved gut health and minimal impact on growth and efficiency of broilers fed select grain sorghum varieties when challenged with EM/CP.

Apparent metabolizable energy, growth performance and carcass traits of Japanese quail fed select modern grain sorghum varieties.

OBJECTIVE: This study was performed to determine the apparent metabolizable energy (AMEn) content of tannin-free red/bronze, white/tan and U.S. No. 2 varieties of grain sorghum for feeding Japanese quail and validate their nutrient profile by evaluating effects on performance and carcass traits with full-substitution of corn. METHODS: Experiment 1 determined the AMEn content of red/bronze, white/tan, and U.S. No. 2 grain sorghum varieties fed to mixed-sex Japanese quail (Coturnix japonica) (n = 314) at 3 and 6-weeks of age. Analyses were based on a 2×4 factorial treatment design with age and grain types defining the treatments, and a randomized complete block experiment design with cage and trials defining the block. AMEn values were validated by evaluating the performance and carcass traits of Japanese quail (n = 644) from 1 to 40 days of age in Experiment 2 with birds were fed 1 of 4 complete diets. Statistical analyses were conducted on performance data and select individual carcass trait measurements. RESULTS: Determined AMEn values at 3-weeks of age were 3,524±122.03 (red/bronze), 3,252±122.03 (white/tan), and 3,039±123.44 (U.S. No. 2) kcal/kg. At 6-weeks of age, determined AMEn were 3,373±297.35 (red/bronze), 3,279±297.35 (white/tan), and 2,966±298.64 (U.S. No. 2) kcal/kg. Carcass traits showed live body weight (p = 0.0409) and hot carcass weight (p = 0.0234) were greatest in U.S. No. 2; however, carcass yield (p<0.0001) was lowest. No significant differences were observed among treatments for feed intake, feed conversion ratio, breast weight and breast yield (p>0.05). CONCLUSION: These studies demonstrated that tannin-free grain sorghum varieties may be a potential alternative to corn in quail diets while maintaining growth performance and carcass parameters.

Ecological divergence and speciation between lemur (Eulemur) sister species in Madagascar.

Understanding ecological niche evolution over evolutionary timescales is crucial to elucidating the biogeographic history of organisms. Here, we used, for the first time, climate-based ecological niche models (ENMs) to test hypotheses about ecological divergence and speciation processes between sister species pairs of lemurs (genus Eulemur) in Madagascar. We produced ENMs for eight species, all of which had significant validation support. Among the four sister species pairs, we found nonequivalent niches between sisters, varying degrees of niche overlap in ecological and geographic space, and support for multiple divergence processes. Specifically, three sister-pair comparisons supported the null model that niches are no more divergent than the available background region. These findings are consistent with an allopatric speciation model, and for two sister pairs (E. collaris-E. cinereiceps and E. rufus-E. rufifrons), a riverine barrier has been previously proposed for driving allopatric speciation. However, for the fourth sister pair E. flavifrons-E. macaco, we found support for significant niche divergence, and consistent with their parapatric distribution on an ecotone and the lack of obvious geographic barriers, these findings most strongly support a parapatric model of speciation. These analyses thus suggest that various speciation processes have led to diversification among closely related Eulemur species.

Genetic evidence for dispersal by both sexes in the Central American Squirrel Monkey, Saimiri oerstedii citrinellus.

Sex-biased dispersal (SBD) is common in many vertebrates, including primates. However, dispersal patterns in New World primates may vary among closely related taxa or populations in different local environments. Here, we test for SBD in an endangered New World primate, the Central American Squirrel Monkey (Saimiri oerstedii citrinellus). Previous studies of behavioral ecology suggest predominantly female dispersal in S.o. oerstedii in the Southern Pacific region of Costa Rica. However, our genetic data do not support strongly female-biased dispersal in S.o. citrinellus in the Central Pacific region. Our tests for SBD using microsatellite data including comparisons of isolation-by-distance, AI(c) , and F(ST) values between males and females were not significant. Also, we found greater population genetic structure in mitochondrial markers than in microsatellite markers, indicative of predominantly male dispersal. We conclude that both sexes disperse in S.o. citrinellus, and that males probably disperse over longer distances. We discuss how spatial and temporal variation among local populations should be taken into account when studying dispersal patterns and especially sex bias.

Homocysteine remethylation in broilers fed surfeit choline or betaine and varying levels and sources of methionine from eight to twenty-two days of age.

Experiments were conducted to assess the effect of surfeit choline (CHOL) or betaine (BET) on growth performance and homocysteine (HCY) remethylation of young broilers fed graded levels of DL-Met (DLM) or 2-hydroxy-4-(methylthio) butanoic acid (HMB). In Experiment 1, a corn-peanut meal diet deficient in Met (0.25% digestible) and Cys (0.28% digestible) was fed; treatments were formulated to contain graded levels (0, 0.04, or 0.08%) of Met from DLM or 0.04% HMB (adjusted for 88% purity) that were fed in the presence or absence of surfeit isomethyl CHOL (0.25%) or BET (0.28%). In Experiment 2, identical treatments were used, but an additional level of HMB (0.08%) was fed, and the basal diet was adequate in Cys (0.43% digestible). There was no overall effect of CHOL or BET on growth performance in Experiments 1 and 2 (P > 0.05); a significant improvement (P < 0.05) in weight gain and feed efficiency did occur with CHOL and BET addition to the basal diet in Experiment 2. In both experiments, weight gain increased linearly (P < 0.05) with the addition of DLM or HMB. Slope ratio methodology was used to assess HMB efficacy in Experiment 2. In the presence of adequate Cys, HMB efficacy was 81.3%; addition of surfeit BET or CHOL had minimal effect on efficacy. The stable isotope study revealed that CHOL and BET addition to diets deficient in Met and Cys or Met alone increased HCY remethylation. It also showed that CHOL and BET have greater influence on folate-dependent remethylation of HCY (via Met synthase) than on BET-dependent remethylation (via BET-HCY methyltransferase) and that levels of CHOL and BET and type of S amino acid deficiency effect remethylation and HMB efficacy.

Memory antibody responses of broiler and leghorn chickens as influenced by dietary vitamin E and route of sheep red blood cell administration.

Influences of dietary levels of vitamin E fed to hens and their progeny, and routes of SRBC inoculation on antibody responses of diverse populations of chickens were studied. Populations were a commercial broiler sire line (C), 2 commercial broiler dam lines (A and B), and Leghorn lines selected for high (H) or low (L) antibody response to SRBC. Dams from lines A and B were fed diets supplemented with either 10 or 300 IU/kg of vitamin E, whereas dams from lines H and L received only the diet with 10 IU of vitamin E/kg. Progeny from matings of C males with A and B females as well as H and L females mated to males from their respective lines were hatched on the same day and fed diets supplemented with either 10 or 300 IU/kg of vitamin E. Breeders were the same age and eggs were incubated in the same machine. Chicks were inoculated on d 14 intravenously with 0.1 mL of a 0.5% suspension of SRBC or intramuscularly with 0.1 mL of a 25% suspension of SRBC. Antibody response was measured 6 and 14 d later. Chicks received a booster i.m. inoculation of 0.1 mL of 25% SRBC on d 28. Titers were again measured 6 and 14 d later. Level of vitamin E fed to dams did not affect progeny BW or plasma vitamin E levels. Although titers were higher following i.v. than i.m. inoculation, the degree of difference varied among stocks. Dietary vitamin E level interacted with inoculation route with a greater response to the higher than lower level of vitamin E for i.v., but there was no difference for i.m. There were stock x level of vitamin E and stock x route of inoculation interactions for secondary responses to SRBC inoculation. Stock rankings after the first inoculation were not predictive of the rankings after the second inoculation. The 30-fold increase in dietary level of vitamin E resulted in >12-fold differences in plasma levels of vitamin E. Overall, there was a stock-dependent influence of dietary vitamin E on growth and humoral antibody response.

Homocysteine remethylation in young broilers fed varying levels of methionine, choline, and betaine.

Methionine is critical in amino acid nutrition for chickens, yet details of the flux of Met metabolites in the avian system are lacking. This study explored the interactions among dietary choline (CHO), betaine (BET), and sulfur amino acid levels on growth and hepatic homocysteine (HCY) remethylation. Graded levels (0, 0.07, 0.11, and 0.24%) of DL-Met were added to diets adequate in CHO and deficient in sulfur amino acids (0.26% digestible Met, 0.26% digestible Cys). Each Met level was tested alone or with the addition of CHO (0.25%) or BET (0.28%). Broilers were reared from 8 to 22 d in raised wire floor battery cages, and the 12 dietary treatments were fed to 3 replicate pens containing 5 birds per pen. Weight gain and feed efficiency were maximized (P < 0.05) with addition of 0.11% supplemental Met, whereas feed intake was maximized (P < 0.05) with addition of 0.07% supplemental Met. Overall, growth parameters were not affected (P > 0.05) by CHO or BET addition. Hepatic tissue primed by the different dietary treatments was subjected to a newly developed stable isotope methodology and HPLC-mass spectrometry to quantify the impact of diet on HCY remethylation. Dietary Met level did not (P > 0.05) affect HCY remethylation, but remethylation through the Met synthase pathway was increased (P < 0.05) by addition of CHO or BET to diets containing deficient or excess levels of Met. Minimal changes in hepatic HCY remethylation through the betaine-homocysteine methyltransferase pathway occurred in response to dietary changes; therefore, data failed to support previous suggestions that BHMT might have a regulatory role when diets containing deficient or excess Met levels are fed. In contrast to previous suppositions based on enzyme activity, under most dietary conditions, the quantity of HCY remethylated by Met synthase appeared to exceed that remethylated by the alternate betaine-homocysteine methyltransferase pathway.

Variation in how mothers, health visitors and general practitioners use the personal child health record.

BACKGROUND: In the UK, a national personal child health record (PCHR) with local adaptations is in widespread use. Previous studies report that parents find the PCHR useful and that health visitors use it more than other health professionals. This study was carried out in Nottingham, where the local PCHR is similar to the national PCHR. OBJECTIVES: To explore variation in use of the PCHR made by mothers with differing social characteristics, to compare heath visitors' and general practitioners' (GPs') use of the PCHR, and to compare health visitors' and GPs' perceptions of the PCHR with those of mothers for whose children they provide care. METHODS: Questionnaires to 534 parents registered with 28 general practices and interviews with a health visitor and GP at each practice. A score per mother for perceived usefulness of the PCHR was developed from the questionnaire, and variation in the score was investigated by linear regression adjusted for clustering. RESULTS: Four hundred and one (75%) questionnaires were returned. Three hundred and twenty-five (82%) mothers thought the PCHR was very good or good. Higher scores for usage of the PCHR were significantly associated with teenage and first-time mothers, but no association was found with mother's social class, education or being a single parent. There was no association between variation in the score and practice, health visitor or GP characteristics. Mothers, health visitors and GPs reported that mothers took the PCHR to baby clinic more frequently than when seeing their GP, and that health visitors wrote in the PCHR more frequently than GPs. Eighteen (67%) health visitors and 20 (71%) GPs said they had difficulty recording information in the PCHR. CONCLUSION: The PCHR is used by most mothers and is important for providing health promotion material to all families with young children. It may be particularly useful for first-time and teenage mothers.

Assessing the quality of preschool child health surveillance in primary care: a pilot study in one health district.

OBJECTIVE: To develop a framework for measuring the quality of preschool child health surveillance acceptable to primary health care teams and measurable at individual primary health care team level. DESIGN: Published research evidence was identified and criteria developed by a local multidisciplinary expert group. The criteria were discussed with primary health care teams. How well they were achieved was assessed. SETTING: Twenty-eight general practices in one health authority area. METHODS: Data collection included observation of baby clinics, interviews with health visitors and general practitioners (GPs), questionnaires to parents, collation of child health surveillance reviews recorded in personal child health records and immunization rates. RESULTS: The criteria for assessing preschool child health surveillance were thought to be acceptable and achievable by primary health care teams. Fifteen of the 22 criteria used to assess baby clinics were met by over 90 of practices. Almost all practices completed child health surveillance reviews within a standard time and achieved 90 coverage for primary immunizations. At many practices, less than 90 of parents felt welcome at the baby clinic, usually had enough time to talk to their health visitor or had the purpose or results of the 6 to 8 week child health surveillance review explained to them. GPs were less likely than health visitors to discuss health promotion at child health surveillance reviews (chi(2) 11.52, P = 0.0007). Few practices had a call-up and recall system for all reviews. CONCLUSION: The framework that we developed for assessing preschool child health surveillance was acceptable and achievable by primary health care teams.

Community paediatrics moves on--an analysis of changing work patterns 1994-97.

OBJECTIVES: To describe the current clinical workload of the modern community paediatrician; to outline the changes in this role over recent years and examine the reasons for these. DESIGN AND SETTING: The design is a retrospective analysis of data routinely collected for contracting purposes. Nottingham community paediatric service 1994-97 is the setting. MAIN OUTCOME MEASURES: These are the characteristics of patients seen, sources of referral, locations of clinical contact, referral rates by area. RESULTS: 36,710 appointments were offered over the time period studied. The non-attendance rate was 17%. Pre-school children made up the largest group seen. Most referrals were from health visitors (23%) and school nurses (29%). There has been a small but significant increase in the numbers of children seen who have developmental problems and disability, and due to child protection issues between the time periods. There has been a shift in the proportions of children seen in a local health centre referral clinic (9.8% increase during 1994-97) rather than in a school setting. Twice as many children are referred to the community paediatrician from inner city areas than from the surrounding county areas. CONCLUSION: The transfer of child health surveillance to the primary health care team and the increased training of community paediatricians, has resulted in community paediatricians developing an increasingly specialised role particularly in the areas of child growth and development, disability, social and behavioural paediatrics.

Assistive technology: what and how for persons with spinal cord injury.

Assistive Technology (AT) is used to "increase, maintain or improve functional capabilities of individuals with disabilities." (Assistive Technology Act of 1998). This term includes durable medical equipment and rehabilitation technology. More limited definitions of AT sometimes do not consider the broad life areas encountered by people with disabilities. To appropriately assess AT needs, the following questions should be considered: a) what does the patient want to do; b) what are the patient's abilities; and c) what technological features need to be included or provided to enhance the patient's abilities? Each question should be considered in the life areas of home, school, community, and vocation. Agencies and organizations that provide AT information include Assistive Technology Act of 1998 programs in each state and territory, vendors, and local county governments. The overall goal of AT is to help an individual meet independence goals in various life areas affected by disability.

Reduced fetal movements and maternal medication - new pregnancy risk factors for neurodevelopmental disability in childhood.

A case-control study was undertaken of 471 children on the Nottingham Special Needs Register (SNR) who were born in one of the two maternity units in the city between 1987 and 1993 (inclusive). Controls were selected as the next infant born at the same hospital following each index case. The aim of the study was to identify risk factors on the Nottingham Obstetric Database for a baby subsequently appearing on the SNR. Disability was analysed by both ICD-9 coding and functional assessment. Factors which independently and significantly predicted a child's likelihood of being on the SNR were breech presentation (adjusted odds ratio (OR) = 4.0), congenital abnormality (OR=4.9), intrapartum fetal distress (OR=1.7), fetal growth restriction (OR=2.0), socioeconomic deprivation (OR=1.8), prematurity (OR=2.2), reduced fetal movements (OR=2.5) and medication in pregnancy (OR=10.4). To our knowledge the last two factors have not previously been reported as risk predictors for neurodevelopmental disability.

Are child health surveillance reviews just routine examinations of normal children?

BACKGROUND: The provision and content of child health surveillance (CHS) has changed greatly since 1990. However, its value continues to be questioned. The introduction of the personal child health record (PCHR) has provided a new means of collecting data about CHS. AIM: To identify what problems are recorded at CHS reviews in the PCHR during the first year of life, and what follow-up/referrals result directly from these reviews. METHOD: A total of 28 practices were recruited from one health authority. All babies born to mothers registered with study practices during one year were followed up. Health visitors returned copies of CHS reviews recorded in children's PCHRs. Written comments on returned reviews were analysed. RESULTS: In all, 2308 babies were entered into the study and 2001 (87%) were followed up for one year. A total of 7848 (78%) CHS reviews were returned. Physical problems were recorded in 58% of children at the 10-14 day, 35% at the six to eight week, and 39% at the six to nine month review. Of physical problems recorded at CHS reviews, 30% required follow-up in primary care and 7% required referral to hospital. Other problems were recorded less frequently and health promotion was recorded at only 7.5% of CHS reviews. CONCLUSION: Child health surveillance provides important opportunities to discuss problems that may cause parental concern and to identify children requiring treatment or follow-up. The design and use of the PCHR needs to change to reflect increasing emphasis on health promotion.

Carbon monoxide--does fetal exposure cause sudden infant death syndrome?

The epidemiological features of sudden infant death syndrome (cot death) include a peak incidence between 8 and 13 weeks of age, a time of death or conception occurring during the winter months and an excess of deaths in infants born to young multiparous women of low socioeconomic status who smoke. We suggest that, through hypoxia, carbon monoxide in tobacco smoke and in the home exerts a noxious effect on the developing central respiratory control mechanism of the fetal brain which then remains particularly susceptible to further insults in the early postnatal period from infection and hyperthermia, resulting in death from central respiratory dysfunction.

Association of mineral composition of neonatal intravenous feeding solutions and metabolic bone disease of prematurity.

To assess the effects of increasing the mineral content of parenteral nutrition solutions on the biochemical and radiological indicators of metabolic bone disease of prematurity 27 neonates who required parenteral nutrition were sequentially allocated to receive either a standard solution (group 1) or one with an increased mineral content (group 2). The 13 patients in group 1 received 0.68 mmol/kg/day of calcium and 0.61 mmol/kg/day of phosphorus, and the 14 in group 2 received 1.25 and 1.20 mmol/kg/day, respectively. The two groups did not differ significantly in the severity of their illness measured by birth weight, gestational age, duration of parenteral nutrition or ventilation, or the amount of supplementary oxygen required. In patients in group 2 the median plasma phosphate concentration was higher, the plasma alkaline phosphatase activity was lower, and there was less radiological evidence of rickets. There were no complications caused by excess calcium and phosphorus, and the rate of growth was similar in both groups. We conclude that an increased mineral content in parenteral nutrition solutions reduces the severity of metabolic bone disease in sick infants who require this form of nutrition.

Effects of dietary protein and added fat on turkeys varying in strain, sex, and age. 1. Live characteristics.

An experiment was conducted to determine the effects of dietary protein and added fat on body weight, feed consumption, and feed efficiency of male and female Nicholas Large White (N), Jaindl Large White (JL), and Jaindl Medium White (JM) turkeys at various ages. At 8 wk of age, 162 males and 288 females from each strain were divided equally into six pens of males and six pens of females per strain. Each of 6 pelleted diets containing standard or high protein and 0, 5, or 10% added fat was fed to one pen of each sex and strain of turkeys. At 20 wk of age, N, JL, and JM males weighed 14.77, 11.56, and 9.00 kg, respectively, and females weighed 8.97, 7.49, and 5.75 kg, respectively. At 28 wk of age, N, JL, and JM males weighed 18.32, 15.10, and 11.65 kg, respectively, and females weighed 10.35, 9.12, and 7.04 kg, respectively. The feeding of diets containing 10% rather than 0% added fat from 8 to 20 wk of age decreased feed consumption 11.1 and 13.1% and increased feed efficiency 16.3 and 16.4% in males and females, respectively. Feeding high protein diets significantly increased body weight gains. The apparent smaller increase than previously experienced in feed efficiency from dietary added fat may be associated with the use of pelleted feed in this experiment.

Effects of dietary protein and added fat on turkeys varying in strain, sex, and age. 2. Carcass characteristics.

The effects of dietary protein and added fat on carcass characteristics were determined at various ages for female and male turkeys and from three strains: Nicholas Large White (N), Jaindl Large White (JL), and Jaindl Medium White (JM). A total of 1,350 turkeys was divided into 36 pens and fed diets containing 0, 5, or 10% added fat and standard or high protein levels from 8 wk of age to time of processing. One-fourth of the females and one-third of the males from each pen were processed at 16, 20, 24, or 28 wk and at 20, 24, or 28 wk, respectively. Breast meat weight, expressed as a percentage of carcass weight, was greater for JM and JL turkeys than for N turkeys (34.6, 33.9, and 31.7%, respectively), increased with age (31.9, 34.1, and 34.2% at 20, 24, and 28 wk of age, respectively), was greater for females than for males (34.2 and 32.6%, respectively), decreased with added fat (33.7, 33.4, and 33.1% for 0, 5, and 10% fat, respectively) and increased with a 3% addition of dietary protein (33.2 and 33.6%, respectively).

Effects of varying fat and protein in diets of growing large white turkeys. 1. Body weights and feed efficiencies.

An experiment was conducted to determine the effects of varying levels of dietary fat and protein in corn-soybean meal diets on body weight gain, feed consumption, and feed efficiency (gain:feed ratio) of Nicholas Large White male turkeys between 8 and 22 wk of age. Diets containing 0, 5, 10, or 15% added fat from one of three sources and 18, 21, or 24% protein were fed. At 12 and 16 wk of age, protein levels of all diets were decreased by 2%. A total of 917 turkeys in 36 pens was used. Additions of 5, 10, and 15% fat increased body weight gains 3.3, 7.5, and 6.0%, respectively. Feed consumption decreased 1.3% and feed efficiency increased 2.1% for each 1% fat added to the standard and high protein diets. The fat containing 15% free fatty acids and 1.5% moisture, impurities, and unsaponifiables (MIU) decreased feed consumption 1.5% and increased feed efficiency 2.3% for each 1% added fat, and the two fat sources containing 48% free fatty acids and 5% MIU decreased feed consumption 1.2 or 1.1% and increased feed efficiency 2.1 or 2.0% for each 1% added fat. The former fat source was estimated to contain 7.7% more metabolizable energy than the average of the latter two fat sources. Cumulative body weight gain, feed consumption, and feed efficiency decreased 13.2, 5.7, and 8.1%, respectively, from feeding low protein diets in comparison with results of feeding standard and high protein diets. Maximum increases in body weight gains were obtained with 10% added fat at all levels of protein.(ABSTRACT TRUNCATED AT 250 WORDS)

Arthroscopic subacromial decompression. An anatomical study.

Anterior acromioplasty as described by Neer has been an effective procedure for shoulder impingement syndrome. Recent presentations by Ellman suggest that an effective acromioplasty may be performed arthroscopically. These clinical reports have not been supported by any laboratory experience. The purpose of our study was to examine the feasibility and attempt to quantitate the results of arthroscopic subacromial decompressions. Six acromioplasties were performed according to the recommended technique of Dr. Neer to create a standard for comparison. Fourteen fresh postmortem specimens were studied. In seven shoulders a standard acromioplasty was performed with an osteotome. In seven shoulders an acromioplasty was performed using standard arthroscopic approaches and motorized instruments. In five shoulders an isolated division of the coracoacromial ligament was performed arthroscopically. The coracoacromial ligament was completely divided in all five cases. In the osteotome group adequate bone was resected in 75% (21/28) measured locations. In the arthroscopic group adequate bone was removed at 86% (24/28) location. This difference is not statistically significant. In the cadaver, anterior acromioplasty was performed effectively and predictably with arthroscopic instruments. This compared favorably to a conventional osteotome acromioplasty. It was concluded that coracoacromial ligament division can be accomplished.

Deficient amino acids in protein of dehulled soybean meal for young turkeys.

Two 12-day experiments were conducted with Large White turkeys to determine which amino acids are deficient in a diet containing dehulled soybean meal as the sole source of protein. A 22% protein basal diet composed of 43.3% glucose monohydrate, 45.4% dehulled soybean meal, .5% DL-methionine, 6% stabilized fat, and added minerals and vitamins served as the negative control. Two positive control diets were formed by substituting either 16.5% dehulled soybean meal or a mixture containing amounts of essential amino acids equivalent to those in the added dehulled soybean meal in place of an equal amount of glucose monohydrate in the basal diet. Nine additional diets were formed by removing one or more amino acids from the mixture. Each of the 12 diets in a block design was fed to two pens of males and two pens of females with 8 birds per pen from 7 to 19 days of age in each experiment. Average body weight gain of poults fed the 22% protein diet with added amino acids approached that of poults fed the 30% protein diet (288 vs. 300 g, respectively). Removal of the amino acid mixture from the 22% protein diet depressed body weight gain by 19.0%. Depressions of 19, 16, 11, 7, and 6% in body weight gains resulted from the removal of valine, threonine, lysine, phenylalanine (or tyrosine or glycine), and isoleucine, respectively. A decrease of 5% was required for significance (P less than or equal to .05). When evaluated by this deletion technique, effects of valine and threonine deficiency were more pronounced than effects of lysine deficiency in dehulled soybean meal for young turkeys.