In situ observations and modelling revealed environmental factors favouring occurrence of Vibrio in microbiome of the pelagic Sargassum responsible for strandings.

Historically, pelagic Sargassum were only found in the Sargasso Sea. Since 2011, blooms were regularly observed in warmer water, further south. Their developments in Central Atlantic are associated with mass strandings on the coasts, causing important damages and potentially dispersion of new bacteria. Microbiomes associated with pelagic Sargassum were analysed at large scale in Central Atlantic and near Caribbean Islands with a focus on pathogenic bacteria. Vibrio appeared widely distributed among pelagic Sargassum microbiome of our samples with higher occurrence than previously found in Mexico Gulf. Six out the 16 Vibrio-OTUs (Operational Taxonomic Unit), representing 81.2 ± 13.1% of the sequences, felt in cluster containing pathogens. Among the four different microbial profiles of pelagic Sargassum microbiome, Vibrio attained about 2% in two profiles whereas it peaked, in the two others, at 6.5 and 26.8% respectively, largely above the concentrations found in seawater surrounding raft (0.5%). In addition to sampling and measurements, we performed backward Lagrangian modelling of trajectories of rafts, and rebuilt the sampled rafts environmental history allowing us to estimate Sargassum growth rates along raft displacements. We found that Vibrio was favoured by high Sargassum growth rate and in situ ammonium and nitrite, modelled phosphate and nitrate concentrations, whereas zooplankters, benthic copepods, and calm wind (proxy of raft buoyancy near the sea surface) were less favourable for them. Relations between Vibrio and other main bacterial groups identified a competition with Alteromonas. According to forward Lagrangian tracking, part of rafts containing Vibrio could strand on the Caribbean coasts, however the strong decreases of modelled Sargassum growth rates along this displacement suggest unfavourable environment for Vibrio. For the conditions and areas observed, the sanitary risk seemed in consequence minor, but in other areas or conditions where high Sargassum growth rate occurred near coasts, it could be more important.

From In Situ to satellite observations of pelagic Sargassum distribution and aggregation in the Tropical North Atlantic Ocean.

The present study reports on observations carried out in the Tropical North Atlantic in summer and autumn 2017, documenting Sargassum aggregations using both ship-deck observations and satellite sensor observations at three resolutions (MSI-10 m, OLCI-300 m, VIIRS-750 m and MODIS-1 km). Both datasets reported that in summer, Sargassum aggregations were mainly observed off Brazil and near the Caribbean Islands, while they accumulated near the African coast in autumn. Based on in situ observations, we propose a five-class typology allowing standardisation of the description of in situ Sargassum raft shapes and sizes. The most commonly observed Sargassum raft type was windrows, but large rafts composed of a quasi-circular patch hundreds of meters wide were also observed. Satellite imagery showed that these rafts formed larger Sargassum aggregations over a wide range of scales, with smaller aggregations (of tens of m2 area) nested within larger ones (of hundreds of km2). Match-ups between different satellite sensors and in situ observations were limited for this dataset, mainly because of high cloud cover during the periods of observation. Nevertheless, comparisons between the two datasets showed that satellite sensors successfully detected Sargassum abundance and aggregation patterns consistent with in situ observations. MODIS and VIIRS sensors were better suited to describing the Sargassum aggregation distribution and dynamics at Atlantic scale, while the new sensors, OLCI and MSI, proved their ability to detect Sargassum aggregations and to describe their (sub-) mesoscale nested structure. The high variability in raft shape, size, thickness, depth and biomass density observed in situ means that caution is called for when using satellite maps of Sargassum distribution and biomass estimation. Improvements would require additional in situ and airborne observations or very high-resolution satellite imagery.

Tunable UV source based on an LED-pumped cavity-dumped Cr:LiSAF laser.

We developed a light-emitting diode (LED)-pumped Cr:LiSAF laser operating in Q-switched and cavity-dumped regimes. The laser produces 1.1 mJ pulses with a pulse duration of 8.5 ns at a repetition rate of 10 Hz on a broad spectrum centered at 840 nm with a full width at half maximum of 23 nm. After frequency tripling in two cascaded LBO crystals, we obtained 7 ns pulses with an energy of 13 µJ at 280 nm and with a spectral width of 0.5 nm, limited by the spectral acceptance of the phase matching process. By rotating both LBO crystals, UV emission is tuned from 276 nm to 284 nm taking advantage of the broad infrared spectrum of the Cr:LiSAF laser.

LED-pumped passively Q-switched Cr:LiSAF laser.

We report, to the best of our knowledge, the first light-emitting diode (LED)-pumped Cr:LiSAF laser, in both quasi-continuous-wave (QCW) and passively Q-switched operation. This Letter is based on the recent development of LED-pumped luminescent concentrators (LCs). Combining the capacity of high-density integration of blue LEDs with the excellent properties of Ce:YAG LCs, this new pump source can deliver high irradiance (7.3 kW/cm2) in the visible to pump Cr:LiSAF. The Cr:LiSAF laser demonstrates an energy of 8.4 mJ at 850 nm in QCW (250 µs pulses at 10 Hz). A small signal gain per roundtrip of 1.44 at 850 nm and a wavelength tunability between 810 and 960 nm have been performed. A passively Q-switched oscillator is also presented using a Cr:YAG saturable absorber. A peak power of 3.1 kW is obtained with a pulse energy of 130 µJ and duration of 41.6 ns.

New LED-based high-brightness incoherent light source in the SWIR.

The first LED-pumped luminescent concentrator (LC) emitting in the short-wave infrared (SWIR) is reported. Low cost LEDs (at 940 nm) are used to pump a Yb,Er:Glass LC emitting at 1550 nm. The optical conversion efficiency of the system is optimized and studied in detail for several optical configurations. A total of 128 LEDs having an emitting surface of 1 mm2 and an irradiance of 51.6 W/cm2, corresponding to a total pump power of 66 W, are used. Optimizing the output power out of a 100-mm-long LC in a continuous wave regime, a power of 850 mW is extracted from the 2.5 x 2 mm2 LC emitting surface area. The optical efficiency is then 1.29%. The performance of this luminescent concentrator is higher by one order of magnitude in term of radiance compared to an LED emitting at the same wavelength.

LED-pumped alexandrite laser oscillator and amplifier.

Taking advantage of light-emitting-diode (LED) performance breakthrough driven by the lighting market, we report, to the best of our knowledge, the first LED-pumped chromium-doped crystal laser oscillator and amplifier based on alexandrite crystals (Cr3+:BeAl2O4). We developed a Ce:YAG concentrator as the pumped source, illuminated by blue LEDs that can be easily power scaled. With 2200 LEDs (450 nm), the Ce:YAG concentrator can deliver to the gain medium up to 268 mJ at 10 Hz at 550 nm with a irradiance of 8.5 kW/cm2. We demonstrate, in oscillator configuration, an LED-pumped alexandrite laser delivering an energy of 2.9 mJ at 748 nm in free running operation. In the cavity, we measured a double-pass small signal gain of 1.28, which is in good agreement with numerical simulations. As an amplifier, the system demonstrated to boost a CW Ti:sapphire laser by a factor of 4 at 750 nm in eight passes with a large tuning range from 710 nm to 800 nm.

Identifying the drivers of abundance and size of the invasive ctenophore Mnemiopsis leidyi in Northwestern Mediterranean lagoons.

Acknowledged as among the worst invasive marine species, Mnemiopsis leidyi has spread through European Seas since the mid-1980's. Here we report a bimonthly survey conducted in 2010-11 in three lagoons (Bages-Sigean, Thau and Berre) and at two adjacent coastal stations (Sète and SOMLIT-Marseille) along the French Mediterranean coast. M. leidyi was present only in Berre and Bages-Sigean with maximum abundances observed in late summer. M. leidyi adults were present year round in Berre with the largest organisms (∼6 cm) observed in April. In Bages-Sigean, they occurred in sufficient abundance to be recorded by fishermen between August and November. Multiple linear regressions highlighted that abundance in both lagoons was mainly influenced by direct effects of salinity and chlorophyll-a, and temperature to a lesser extent. While M. leidyi has not yet been recorded in Thau, the lagoon is continually monitored to detect the potential establishment of M. leidyi.

Revisiting of LED pumped bulk laser: first demonstration of Nd:YVO4 LED pumped laser.

We describe here what is, to the best of our knowledge, the first LED pumped Nd:YVO4 laser. Near-IR LED arrays with a wavelength centered close to 850 nm were used to pump transversely the crystal. By pulsing LEDs, with a duration of the order of the laser transition lifetime, we obtained sufficient pump intensities to reach the laser threshold. At a frequency of 250 Hz, we obtained an output energy of 40 µJ at 1064 nm for an input pump energy of 7.4 mJ, which corresponds to an optical efficiency of 0.5%. Experimental results of small signal gain are compared with theoretical analysis.

Man-induced hydrological changes, metazooplankton communities and invasive species in the Berre Lagoon (Mediterranean Sea, France).

The Berre Lagoon has been under strong anthropogenic pressure since the early 1950s. The opening of the hydroelectric EDF power plant in 1966 led to large salinity drops. The zooplankton community was mainly composed of two common brackish species: Acartia tonsa and Brachionus plicatilis. Since 2006, European litigation has strongly constrained the input of freshwater, maintaining the salinity above 15. A study was performed between 2008 and 2010 to evaluate how these modifications have impacted the zooplankton community. Our results show that the community is more diverse and contains several coastal marine species (i.e., Centropages typicus, Paracalanus parvus and Acartia clausi). A. tonsa is still present but is less abundant, whereas B. plicatilis has completely disappeared. Strong predatory marine species, such as chaetognaths, the large conspicuous autochtonous jellyfish Aurelia aurita and the invasive ctenophore Mnemiopsis leidyi, are now very common as either seasonal or permanent features of the lagoon.

Incongruence between morphotypes and genetically delimited species in the coral genus Stylophora: phenotypic plasticity, morphological convergence, morphological stasis or interspecific hybridization?

BACKGROUND: Morphological data suggest that, unlike most other groups of marine organisms, scleractinian corals of the genus Stylophora are more diverse in the western Indian Ocean and in the Red Sea than in the central Indo-Pacific. However, the morphology of corals is often a poor predictor of their actual biodiversity: hence, we conducted a genetic survey of Stylophora corals collected in Madagascar, Okinawa, the Philippines and New Caledonia in an attempt to find out the true number of species in these various locations. RESULTS: A molecular phylogenetic analysis of the mitochondrial ORF and putative control region concurs with a haploweb analysis of nuclear ITS2 sequences in delimiting three species among our dataset: species A and B are found in Madagascar whereas species C occurs in Okinawa, the Philippines and New Caledonia. Comparison of ITS1 sequences from these three species with data available online suggests that species C is also found on the Great Barrier Reef, in Malaysia, in the South China Sea and in Taiwan, and that a distinct species D occurs in the Red Sea. Shallow-water morphs of species A correspond to the morphological description of Stylophora madagascarensis, species B presents the morphology of Stylophora mordax, whereas species C comprises various morphotypes including Stylophora pistillata and Stylophora mordax. CONCLUSIONS: Genetic analysis of the coral genus Stylophora reveals species boundaries that are not congruent with morphological traits. Of the four hypotheses that may explain such discrepancy (phenotypic plasticity, morphological stasis, morphological convergence, and interspecific hybridization), the first two appear likely to play a role but the fourth one is rejected since mitochondrial and nuclear markers yield congruent species delimitations. The position of the root in our molecular phylogenies suggests that the center of origin of Stylophora is located in the western Indian Ocean, which probably explains why this genus presents a higher biodiversity in the westernmost part of its area of distribution than in the "Coral Triangle".