ABSTRACT
Alcohol abuse has a high impact on the mortality and morbidity related to a great number of diseases and is responsible for the development of alcoholic liver disease (ALD). It remains challenging to detect and evaluate its severity, which is crucial for prognosis. In this work, we studied if urinary EVs (uEVs) could serve in diagnose and evaluate cirrhosis in ALD. To this purpose, uEVs characterization by cryo-electron microscopy (Cryo-EM), Nanoparticle Tracking Analysis (NTA) and Western blotting (WB) was performed in a cohort of 21 controls and 21 cirrhotic patients. Then, proteomics of uEVs was carried out in a second cohort of 6 controls and 8 patients in order to identify new putative biomarkers for cirrhosis in ALD. Interestingly, uEVs concentration, size and protein composition were altered in cirrhotic patients. From a total of 1304 proteins identified in uEVs, 90 of them were found to be altered in cirrhotic patients. The results suggest that uEVs could be considered as a tool and a supplier of new biomarkers for cirrhosis in ALD, whose application would be especially relevant in chronic patients. Yet, further research is necessary to obtain more relevant result in clinical terms.
Subject(s)
Extracellular Vesicles/metabolism , Liver Cirrhosis , Liver Diseases, Alcoholic , Urinalysis/methods , Urinary Tract/metabolism , Biomarkers/metabolism , Blotting, Western/methods , Cryoelectron Microscopy/methods , Early Diagnosis , Humans , Liquid Biopsy/methods , Liver Cirrhosis/diagnosis , Liver Cirrhosis/etiology , Liver Cirrhosis/urine , Liver Diseases, Alcoholic/complications , Liver Diseases, Alcoholic/diagnosis , Liver Diseases, Alcoholic/urine , Male , Middle Aged , Pilot Projects , Proteomics/methods , Proteomics/trends , Reproducibility of ResultsABSTRACT
No disponible
Subject(s)
Humans , Female , Middle Aged , Echinococcosis, Hepatic/diagnostic imaging , Echinococcosis, Hepatic/surgery , Albendazole/administration & dosage , Procalcitonin , Abdomen/diagnostic imaging , Abdomen/pathology , Duodenum/pathology , Duodenum/surgery , Echinococcosis, Hepatic/complicationsABSTRACT
No disponible
Subject(s)
Humans , Female , Adult , Common Variable Immunodeficiency/complications , Cytomegalovirus Infections/diagnosis , Cytomegalovirus Infections/drug therapy , Infliximab/therapeutic use , B-Lymphocytes , T-Lymphocytes , Tumor Necrosis Factor-alpha/therapeutic useABSTRACT
No disponible
