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1.
Biomater Sci ; 11(12): 4073-4093, 2023 Jun 13.
Article in English | MEDLINE | ID: mdl-37060109

ABSTRACT

A painless and non-invasive method to deliver drugs using dermal and transdermal administration routes has been expanding for more than 30 years as it reduces the risk of drug overdoses that can be associated with oral administrations or injections. To understand the particularities of this drug delivery pathway, we will present a rapid review of the skin, including its structure and the parameters that influence drug diffusion into it, and then discuss the strategies that improve dermal drug delivery. Of the multitude of existing systems used for topical dermal and transdermal applications, this review will focus on the breakthroughs in drug delivery systems made of hydrogels. Specifically, we will firstly present the use of hydrogels as innovative drug delivery vehicles to carry the active ingredient and penetrate the skin barrier. We will discuss the structure of hydrogels and the physicochemical parameters to master for improving drug delivery, as well as the drug encapsulation and release processes from hydrogels. In the last part, we will review the use of hydrogels as pharmaceutical forms associated with other vehicles - as emulsions, lipid nanoparticles, vesicles, capsules and polymeric or inorganic nanoparticles - suitable for skin penetration enhancement and drug protection, as well as side effects that may limit their use.


Subject(s)
Hydrogels , Skin Absorption , Administration, Cutaneous , Hydrogels/chemistry , Skin , Drug Delivery Systems , Excipients
2.
Int J Mol Sci ; 24(6)2023 Mar 20.
Article in English | MEDLINE | ID: mdl-36982939

ABSTRACT

The impact of the polyunsaturated fatty acids (PUFAs) at physiological concentrations on the composition of eicosanoids transported within the extracellular vesicles (EVs) of rat bone marrow mesenchymal stem cells and cardiomyoblasts was reported by our group in 2020. The aim of this article was to extend this observation to cells from the cardiac microenvironment involved in the processes of inflammation, namely mouse J774 macrophages and rat heart mesenchymal stem cells cMSCs. Moreover, to enhance our capacity to understand the paracrine exchange between these orchestrators of cardiac inflammation, we investigated some machinery involved in the eicosanoid's synthesis transported by the EVs produced by these cells (including the two formerly described cells: bone marrow mesenchymal stem cells BM-MSC and cardiomyoblasts H9c2). We analyzed the oxylipin and the enzymatic content of the EVs collected from cell cultures supplemented (or not) with PUFAs. We prove that large eicosanoid profiles are exported in the EVs by the cardiac microenvironment cells, but also that these EVs carry some critical and functional biosynthetic enzymes, allowing them to synthesize inflammation bioactive compounds by sensing their environment. Moreover, we demonstrate that these are functional. This observation reinforces the hypothesis that EVs are key factors in paracrine signaling, even in the absence of the parent cell. We also reveal a macrophage-specific behavior, as we observed a radical change in the lipid mediator profile when small EVs derived from J774 cells were exposed to PUFAs. To summarize, we prove that the EVs, due to the carried functional enzymes, can alone produce bioactive compounds, in the absence of the parent cell, by sensing their environment. This makes them potential circulating monitoring entities.


Subject(s)
Extracellular Vesicles , Mice , Rats , Animals , Heart , Fatty Acids, Unsaturated , Eicosanoids , Inflammation
3.
Chem Phys Lipids ; 239: 105113, 2021 09.
Article in English | MEDLINE | ID: mdl-34216586

ABSTRACT

1,8-cineole is a monoterpene commonly used by the food, cosmetic, and pharmaceutical industries owing to its flavor and fragrances properties. In addition, this bioactive monoterpene has demonstrated bactericidal and fungicidal activities. However, such activities are limited due to its low aqueous solubility and stability. This study aimed to develop nanoemulsion containing cineole and assess its stability and antibacterial activity in this context. The spontaneous emulsification method was used to prepare nanoemulsion (NE) formulations (F1, F2, F3, F4, and F5). Following the development of NE formulations, we chose the F1 formulation that presented an average droplet size (in diameter) of about 100 nm with narrow size distribution (PdI <0.2) and negative zeta potential (∼ - 35 mV). According to the analytical centrifugation method with photometric detection, F1 and F5 formulations were considered the most stable NE with lower droplet migration velocities. In addition, F1 formulation showed high incorporation efficiency (> 80 %) and TEM analyses demonstrated nanosized oil droplets with irregular spherical shapes and without any aggregation tendency. Antibacterial activity assessment showed that F1 NE was able to enhance the cineole action against Staphylococcus aureus, Enterococcus faecalis, and Streptococcus pyogenes. Therefore, using a simple and reproducible method of low energy emulsification we designed a stable nanoemulsion containing 1,8-cineole with improved antibacterial activity against Gram-positive strains.


Subject(s)
Anti-Bacterial Agents/chemistry , Emulsions/chemistry , Eucalyptol/chemistry , Nanostructures/chemistry , Anti-Bacterial Agents/pharmacology , Drug Stability , Enterococcus faecalis/drug effects , Eucalyptol/pharmacology , Microbial Sensitivity Tests , Particle Size , Staphylococcus aureus/drug effects
4.
Pharmaceutics ; 12(12)2020 Dec 17.
Article in English | MEDLINE | ID: mdl-33348690

ABSTRACT

Skin is our biggest organ. It interfaces our body with its environment. It is an efficient barrier to control the loss of water, the regulation of temperature, and infections by skin-resident and environmental pathogens. The barrier function of the skin is played by the stratum corneum (SC). It is a lipid barrier associating corneocytes (the terminally differentiated keratinocytes) and multilamellar lipid bilayers. This intricate association constitutes a very cohesive system, fully adapted to its role. One consequence of this efficient organization is the virtual impossibility for active pharmaceutical ingredients (API) to cross the SC to reach the inner layers of the skin after topical deposition. There are several ways to help a drug to cross the SC. Physical methods and chemical enhancers of permeation are a possibility. These are invasive and irritating methods. Vectorization of the drugs through nanocarriers is another way to circumvent the SC. This mini-review focuses on supramolecular and macromolecular matrices designed and implemented for skin permeation, excluding vesicular nanocarriers. Examples highlight the entrapment of anti-inflammatory API to treat inflammatory disorders of the skin.

5.
Biochimie ; 178: 69-80, 2020 Nov.
Article in English | MEDLINE | ID: mdl-32835733

ABSTRACT

Recent works reported the relevance of cellular exosomes in the evolution of different pathologies. However, most of these studies focused on the ability of exosomes to convey mi-RNA from cell to cell. The level of knowledge concerning the transport of lipid mediators by these nanovesicles is more than fragmented. The role of lipid mediators in the inflammatory signaling is fairly well described, in particular concerning the derivatives of the arachidonic acid (AA), called eicosanoïds or lipid mediators. The aim of the present work was to study the transport of these lipids within the extracellular vesicles of rat bone marrow mesenchymal stem cells (BM-MSC) and the cardiomyoblast cell line H9c2. We were able to characterize, for the first time, complete profiles of oxilipins within these nanovesicles. We studied also the impact on these profiles, of the polyunsaturated fatty acids (PUFAs) know to be precursors of the inflammatory signaling molecules (AA, eicosapentaenoic acid EPA and Docosahexaenoic acid DHA), at physiological concentrations. By growing the progenitor cells under PUFAs supplementation, we provide a comprehensive assessment of the beneficial effect of ω-3 PUFA therapy. Actually, our results tend to support the resolving role of the inflammation that stromal cell-derived extracellular vesicles can have within the cardiac microenvironment.


Subject(s)
Eicosanoids/chemistry , Eicosanoids/metabolism , Extracellular Vesicles/chemistry , Extracellular Vesicles/metabolism , Mesenchymal Stem Cells/chemistry , Mesenchymal Stem Cells/metabolism , Myoblasts, Cardiac/chemistry , Myoblasts, Cardiac/metabolism , Animals , Bone Marrow/chemistry , Bone Marrow/drug effects , Bone Marrow/metabolism , Cell Line , Extracellular Vesicles/drug effects , Humans , Inflammation/metabolism , Inflammation Mediators/chemistry , Inflammation Mediators/metabolism , Lipid Metabolism , Mesenchymal Stem Cells/drug effects , Myoblasts, Cardiac/drug effects , Oxylipins/chemistry , Oxylipins/metabolism , Rats
6.
Biomolecules ; 10(6)2020 06 23.
Article in English | MEDLINE | ID: mdl-32586038

ABSTRACT

Dendrimers are nanosized, arborescent macromolecules synthesized in a stepwise fashion with attractive degrees of functionality and structure definition. This is one of the reasons why they are widely used for biomedical applications. Previously, we have shown that a poly(phosphorhydrazone) (PPH) dendrimer capped with anionic azabisphosphonate groups (so-called ABP dendrimer) has immuno-modulatory and anti-inflammatory properties towards human immune cells in vitro. Thereafter, we have shown that the ABP dendrimer has a promising therapeutic efficacy to treat models of acute and chronic inflammatory disorders in animal models. In these models, the active pharmaceutical ingredient was administered systematically (intravenous and oral administrations), but also loco-regionally in the vitreous tissue. Herein, we assessed the therapeutic efficacy of the ABP dendrimer in the preclinical mouse model of psoriasis induced by imiquimod. The ABP dendrimer was administered in phosphate-buffered saline solution via either systemic injection or topical application. We show that the topical application enabled the control of both the clinical and histopathological scores, and the control of the infiltration of macrophages in the skin of treated mice.


Subject(s)
Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Dendrimers/therapeutic use , Hydrazones/therapeutic use , Polymers/therapeutic use , Psoriasis/drug therapy , Animals , Anti-Inflammatory Agents, Non-Steroidal/chemical synthesis , Anti-Inflammatory Agents, Non-Steroidal/chemistry , Aza Compounds/chemistry , Aza Compounds/therapeutic use , Dendrimers/chemical synthesis , Dendrimers/chemistry , Diphosphonates/chemistry , Diphosphonates/therapeutic use , Disease Models, Animal , Hydrazones/chemical synthesis , Hydrazones/chemistry , Imiquimod , Male , Mice , Mice, Inbred BALB C , Molecular Structure , Polymers/chemical synthesis , Polymers/chemistry , Psoriasis/chemically induced , Psoriasis/pathology
7.
RSC Adv ; 11(1): 442-451, 2020 Dec 21.
Article in English | MEDLINE | ID: mdl-35423006

ABSTRACT

Dermal/transdermal drug delivery continues to grow in importance as a means of enhancing treatment activity while reducing toxicity by avoiding the systemic absorption of the drug. At the same time, this has led to the adjustment of a wide diversity of drug carriers. This paper begins with a review of the skin, including its structure and the parameters that influence drug diffusion, followed by strategies to improve dermal drug delivery. Of the multitude of existing carriers, we will focus on the most advanced vectors in dermal/transdermal delivery, and in particular, on vesicular systems. This review will present the state of the art as well as the new trends in this domain. Through the description of these systems, we will try to obtain information on the ideal properties that the carrier must have in order to improve the cutaneous and transcutaneous penetration of the drug.

8.
J Mol Cell Cardiol ; 138: 165-174, 2020 01.
Article in English | MEDLINE | ID: mdl-31836542

ABSTRACT

AIMS: Apelin and vitamin E have been proposed as signaling molecules, but their synergistic role is unknown. The aim of this work was to develop vitamin E TPGS/Apelin system to test their cardioprotective and metabolic efficacy in vitro and in vivo. METHODS: FDA-approved surfactant D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS-1000) and Apelin complex were characterized by physico-chemical methods (CMC determination, dynamic light scattering and circular dichroism). In vitro studies were carried out on H9C2 cardiomyoblasts and isolated murine cardiomyocytes. In vivo studies were performed in isoproterenol- and high-fat diet-induced cardiac remodeling models in mice. RESULTS: We found that vitamin E TPGS/Apelin provide cardioprotective and metabolic efficacy in vitro and in vivo. In vitro studies revealed that vitamin E TPGS/Apelin reduces hypoxia-induced mitochondrial ROS production in cultured cardiomyocytes and H9C2 cardiomyoblasts. In addition, vitamin E TPGS/Apelin confers apoptotic response to hypoxic stress in cells. In a mouse model of isoproterenol-induced cardiac injury, TPGS is not able to affect cardiac remodeling, however combination of vitamin E TPGS and Apelin counteracts myocardial apoptosis, oxidative stress, hypertrophy and fibrosis. Furthermore, combination treatment attenuated obesity-induced cardiometabolic and fibrotic remodeling in mice. CONCLUSION: Together, our data demonstrated the therapeutic benefits of vitamin E TPGS/Apelin complex to combat cardiovascular and metabolic disorders.


Subject(s)
Apelin/pharmacology , Cardiotonic Agents/pharmacology , Vitamin E/pharmacology , Animals , Apoptosis/drug effects , Cardiomegaly/complications , Cardiomegaly/pathology , Cell Hypoxia/drug effects , Cell Line , Diabetic Cardiomyopathies/complications , Diabetic Cardiomyopathies/pathology , Diet, High-Fat , Fibrosis , Isoproterenol , Mice, Inbred C57BL , Mitochondria/drug effects , Mitochondria/metabolism , Myocytes, Cardiac/drug effects , Myocytes, Cardiac/metabolism , Oxidative Stress/drug effects , Rats , Reactive Oxygen Species/metabolism , Vascular Remodeling/drug effects
9.
J Chromatogr A ; 1616: 460792, 2020 Apr 12.
Article in English | MEDLINE | ID: mdl-31866137

ABSTRACT

Polyglycerol esters (PGEs), produced by esterification of fatty acids on polyglycerols, were analysed by High Resolution Mass Spectrometry (HRMS), HPLC-MS and U-HPLC-MS. A structural study of PGEs in 4 samples synthesised by the Gattefossé company was carried out using an elemental analysis of HRMS spectra and modelling of all probable isomers and cyclic structures. The results were used to construct a structural database of all species present in the 4 samples. After an assessment of the selectivity of 5 reversed phase columns: Aeris Widepore XB-C8, 3.6 µm, 2.1 × 150 mm (Phenomenex), Acquity CSH C18 1.7 µm 2.1 × 50 mm, Acquity CSH Phenyl-Hexyl 1.7 µm 2.1 × 50 mm, Acquity CSH Fluoro-Phenyl 1.7 µm 2.1 × 50 mm (Waters Co.) and Kinetex F5 1.7 µm 2.1 × 100 mm (Phenomenex), HPLC-MS and U-HPLC-MS analyses were performed on an Aeris Widepore XB-C8 (Phenomenex) column (HPLC) and Acquity CSH Fluoro-Phenyl (Waters) column (U-HPLC) with aqueous formic acid /acetonitrile in gradient mode. The separation was optimised with 10 min (HPLC) and 5 min (U-HPLC) of gradient. The detection, performed on a QDA detector (Waters), produced extracted ion chromatograms (XICs) based on all adducts identified in the HRMS analysis. HPLC and U-HPLC analyses showed the different mono- and di-ester species and provided relative quantification of all identified constituents. The combined analyses of the HRMS, HPLC-MS and U-HPLC-MS results were used to compare the different PGE batches and quantify the molecular constituents according to their relative abundance, for these complex mixtures. With HPLC and U-HPLC analyses, using 2 different gradient times and 2 different selectivity columns, and comparing the retention factors and log P of the different species, it was possible to link structural identification and relative quantification of all PGEs identified in the samples.


Subject(s)
Chromatography, Liquid/methods , Esters/analysis , Esters/chemistry , Glycerol/analysis , Glycerol/chemistry , Mass Spectrometry/methods , Polymers/analysis , Polymers/chemistry , Acetonitriles/chemistry , Chromatography, High Pressure Liquid , Formates/chemistry , Models, Theoretical
10.
Biomolecules ; 9(9)2019 09 11.
Article in English | MEDLINE | ID: mdl-31514434

ABSTRACT

Dendrimers are nanosized, arborescent polymers of which size and structure are perfectly controlled. This is one reason why they are widely used for biomedical purposes. Previously, we showed that a phosphorus-based dendrimer capped with anionic azabisphosphonate groups (so-called ABP dendrimer) has immuno-modulatory and anti-inflammatory properties towards human immune cells in vitro. Thereafter, we have shown that the ABP dendrimer has a promising therapeutic efficacy to treat models of chronic inflammatory disorders. On the way to clinical translation, the biodistribution and the safety of this drug-candidate has to be thoroughly assessed. In this article, we present preliminary non-clinical data regarding biodistribution, hematological safety, genotoxicity, maximal tolerated doses, and early cardiac safety of the ABP dendrimer. One of the genotoxicity assays reveals a potential mutagen effect of the item at a concentration above 200 µM, i.e., up to 100 times the active dose in vitro on human immune cells. However, as the results obtained for all the other assays show that the ABP dendrimer has promising biodistribution and safety profiles, there is no red flag raised to hamper the regulatory pre-clinical development of the ABP dendrimer.


Subject(s)
Anti-Inflammatory Agents/adverse effects , Anti-Inflammatory Agents/pharmacokinetics , Dendrimers/adverse effects , Dendrimers/pharmacokinetics , Hydrazones/chemistry , Safety , Animals , Anti-Inflammatory Agents/chemistry , Dendrimers/chemistry , Female , Mice , Rats , Tissue Distribution
11.
Chemphyschem ; 16(18): 3877-85, 2015 Dec 21.
Article in English | MEDLINE | ID: mdl-26456707

ABSTRACT

We performed a systematic study on the spectroscopic and aggregation properties of stoichiometric mixtures (1:4) of the tetracationic meso-tetrakis(4-N-methylpyridinium)porphyrin (H2 TMPyP) and three sodium alkylsulfate surfactants (tetradecyl, hexadecyl, and octadecylsulfate) in an aqueous solution. The objective was to build a supramolecular aggregate, which would favor the internalization of tetracationic porphyrins in cells without chemical modification of the structure of the porphyrin. We show that stoichiometric H2 TMPyP/alkylsulfate (1:4) mixtures lead to the formation of large hollow spherical aggregates (60-160 nm). The TEM images show that the membrane of these aggregates are composed of smaller aggregates, which are probably rod-like micelles. These rod-like micelles have a hydrophobic core composed of the alkyl chains of the alkylsulfate surfactant, whereas the charged surface corresponds to the tetracationic porphyrins.


Subject(s)
Porphyrins/chemistry , Surface-Active Agents/chemistry , Anions , Cations , Microscopy, Electron, Transmission , Proton Magnetic Resonance Spectroscopy , Water/chemistry
12.
Nanoscale ; 7(42): 17672-84, 2015 Nov 14.
Article in English | MEDLINE | ID: mdl-26335052

ABSTRACT

Dendrimers are nano-materials with perfectly defined structure and size, and multivalency properties that confer substantial advantages for biomedical applications. Previous work has shown that phosphorus-based polyphosphorhydrazone (PPH) dendrimers capped with azabisphosphonate (ABP) end groups have immuno-modulatory and anti-inflammatory properties leading to efficient therapeutic control of inflammatory diseases in animal models. These properties are mainly prompted through activation of monocytes. Here, we disclose new insights into the molecular mechanisms underlying the anti-inflammatory activation of human monocytes by ABP-capped PPH dendrimers. Following an interdisciplinary approach, we have characterized the physicochemical and biological behavior of the lead ABP dendrimer with model and cell membranes, and compared this experimental set of data to predictive computational modelling studies. The behavior of the ABP dendrimer was compared to the one of an isosteric analog dendrimer capped with twelve azabiscarboxylate (ABC) end groups instead of twelve ABP end groups. The ABC dendrimer displayed no biological activity on human monocytes, therefore it was considered as a negative control. In detail, we show that the ABP dendrimer can bind both non-specifically and specifically to the membrane of human monocytes. The specific binding leads to the internalization of the ABP dendrimer by human monocytes. On the contrary, the ABC dendrimer only interacts non-specifically with human monocytes and is not internalized. These data indicate that the bioactive ABP dendrimer is recognized by specific receptor(s) at the surface of human monocytes.


Subject(s)
Anti-Inflammatory Agents/chemistry , Dendrimers/chemistry , Monocytes/metabolism , Anti-Inflammatory Agents/metabolism , Calorimetry, Differential Scanning , Cells, Cultured , Dendrimers/metabolism , Humans , Lipid Bilayers/chemistry , Lipid Bilayers/metabolism , Microscopy, Confocal , Molecular Dynamics Simulation , Monocytes/cytology , Phosphatidylcholines/chemistry , Phosphorus/chemistry
13.
Chemphyschem ; 16(16): 3433-7, 2015 Nov 16.
Article in English | MEDLINE | ID: mdl-26377841

ABSTRACT

The self-association properties of anti-HIV catanionic dendrimers as multivalent galactosylceramide (GalCer)-derived inhibitors are presented. The study was designed to elucidate the origin of the relatively high cytotoxicity values of these anti-HIV catanionic dendrimers, which have previously been found to exhibit in vitro anti-HIV activity in the submicromolar range. The physicochemical properties of these catanionic dendrimers were studied to tentatively correlate the structural parameters with self-association and biological properties. We can conclude from this study that the absence of correlation between the hydrophobicity and the cytotoxicity of the catanionic systems could be explained by the partial segregation of the different partners of the catanionic entities.


Subject(s)
Anti-HIV Agents/chemistry , Dendrimers/chemistry , Anti-HIV Agents/metabolism , Dendrimers/chemical synthesis , Dendrimers/metabolism , Dynamic Light Scattering , Galactosylceramides/chemistry , HIV Envelope Protein gp120/antagonists & inhibitors , HIV Envelope Protein gp120/metabolism , HIV-1/metabolism , Humans , Surface-Active Agents/chemistry
14.
Mol Pharm ; 12(1): 103-10, 2015 Jan 05.
Article in English | MEDLINE | ID: mdl-25310849

ABSTRACT

Lactose-derived catanionic vesicles offer unique opportunities to overcome cellular barriers. These potential nanovectors, very easy to formulate as drug delivery systems, are able to encapsulate drugs of various hydrophilicity. This article highlights versatile interaction mechanisms between these catanionic vesicles, labeled with hydrophilic and amphiphilic fluorescent probes, and a mammalian cell line, Chinese Hamster Ovary. Confocal microscopy and flow cytometry techniques show that these vesicles are internalized by cells through cellular energy dependent processes, as endocytosis, but are simultaneously able to spontaneously fuse with cell plasma membranes and release their hydrophilic content directly inside the cytosol. Such innovative and polyvalent nanovectors, able to deliver their content via different internalization pathways, would positively be a great progress for the coadministration of drugs of complementary efficiency.


Subject(s)
Endocytosis , Membrane Fusion , Membranes, Artificial , Animals , CHO Cells , Cations , Cell Line , Cell Membrane/metabolism , Cell Separation , Cricetulus , Cytosol , Drug Delivery Systems , Flow Cytometry , Fluorescent Dyes/chemistry , Glycolipids/chemistry , Kinetics , Lactose/chemistry , Microscopy, Confocal , Surface-Active Agents
15.
J Colloid Interface Sci ; 401: 155-60, 2013 Jul 01.
Article in English | MEDLINE | ID: mdl-23618324

ABSTRACT

Chloroaluminum phthalocyanine (ClAlPc) is a promising sensitizer molecule for photodynamic therapy, but its hydrophobicity makes it difficult to formulate. In this study, we have efficiently encapsulated ClAlPc into gelled soybean oil particles dispersed in water. 12-Hydroxystearic acid (HSA) and polyethyleneimine (PEI) were the gelling and stabilizing agents, respectively. The preparation process involved hot emulsification above the gelation temperature (Tgel), followed by cooling to room temperature, which gave a colloidal dispersion of gelled particles of oil in aqueous medium. The gelled particles containing ClAlPc had a medium diameter of 280 nm, homogeneous size distribution (polydispersity index ≈0.3) and large positive zeta potential (about +50 mV) and showed a spherical morphology. The gelled oil particle formulations exhibited good physical stability over a 6-month period. ClAlPc interfered with the HSA self-assembly only slightly, and decreased the gelation temperature to a small extent; however it did not affect gelation process of the oil droplets. The amounts of PEI and HSA employed during the preparation allowed us to control particle size and the dispersion stability, a phenomenon that results from complex electrostatic interactions between the positively charged PEI and the negatively charged HSA fibers present on the gelled particles surface. In summary, by using the right ClAlPc, HSA, and PEI proportions, we prepared very stable dispersions of gelled soybean oil particles with excellent ClAlPc encapsulation efficiency. The obtained colloidal formulation of gelled oil particles loaded with ClAlPc shall be very useful for photodynamic therapy protocols.


Subject(s)
Indoles/chemistry , Organometallic Compounds/chemistry , Soybean Oil/chemistry , Hydrophobic and Hydrophilic Interactions , Molecular Structure , Particle Size , Polyethyleneimine/chemistry , Stearic Acids/chemistry , Surface Properties , Temperature
16.
Chemphyschem ; 14(6): 1126-31, 2013 Apr 15.
Article in English | MEDLINE | ID: mdl-23436492

ABSTRACT

Skin deep: A bioactive formulation for dermal delivery of antihistamines is obtained by using the original properties of catanionic associations towards self-assembly in water. The drug, which participates in its own transport, is preserved from photodegradation when solubilised in the bioactive formulation. The drug release through the skin is also delayed.


Subject(s)
Monosaccharides/chemistry , Surface-Active Agents/chemistry , Anti-Allergic Agents/administration & dosage , Drug Carriers/chemistry , Furans/administration & dosage , Humans , Hypersensitivity/drug therapy , Promethazine/administration & dosage , Pyridones/administration & dosage , Skin Absorption
17.
Chem Commun (Camb) ; 48(53): 6648-50, 2012 Jul 07.
Article in English | MEDLINE | ID: mdl-22634609

ABSTRACT

Spontaneous receptor-free membrane fusion with pure lipid systems, used as a cell membrane model, is demonstrated with easy-to-handle lactose-derived catanionic vesicles. This fusion, mediated and controlled by phospholipids, emphasizes the great value of these nanovesicles for enhanced direct cytosolic drug delivery without the shortcomings linked with endocytic pathways.


Subject(s)
Cell Membrane/chemistry , Membrane Fusion , Models, Biological , Nanostructures/chemistry , Unilamellar Liposomes/chemistry , Cations , Molecular Structure , Surface-Active Agents/chemistry , Water/chemistry
18.
Int J Pharm ; 403(1-2): 230-6, 2011 Jan 17.
Article in English | MEDLINE | ID: mdl-20974236

ABSTRACT

Among drug delivery systems, catanionic vesicles now appear as powerful candidates for pharmaceutical applications because they are relatively cheap and easy to use, thus well corresponding to industrial requirements. Using labelled vesicles made of a tricatenar catanionic surfactant, the work reported here aims at exploring the mechanisms by which internalisation into a cell occurs. The study was performed on various cell types such as phagocytic as well as non-phagocytic cells using confocal laser scanning microscopy and flow cytometry. Using various inhibitors, endocytosis and also a passive process, as probably fusion, were highlighted as interaction phenomena between catanionic vesicles and cell membranes. Finally, the interaction modelled with giant liposomes as membrane models confirmed the hypothesis of the occurrence of a fusion phenomenon between the nanovectors and cell membranes. This process highlights the potential of catanionic vesicles for a future pharmaceutical application as a universal drug delivery system.


Subject(s)
Cell Membrane/drug effects , Drug Carriers/chemistry , Surface-Active Agents/chemistry , Animals , Anions/chemical synthesis , Anions/chemistry , Anions/pharmacokinetics , Cations/chemical synthesis , Cations/chemistry , Cations/pharmacokinetics , Cattle , Cell Membrane/metabolism , Cell Survival/drug effects , Cells, Cultured , Chromaffin Cells/drug effects , Chromaffin Cells/metabolism , Drug Carriers/chemical synthesis , Drug Carriers/pharmacokinetics , Drug Delivery Systems/methods , Endocytosis/drug effects , Flow Cytometry , Humans , Keratinocytes/drug effects , Keratinocytes/metabolism , Macrophages/drug effects , Macrophages/metabolism , Membrane Fusion/drug effects , Microscopy, Confocal , Microscopy, Electron, Transmission , Molecular Structure , Particle Size , Spectrometry, Fluorescence , Surface Properties , Surface-Active Agents/chemical synthesis , Surface-Active Agents/pharmacokinetics
19.
Chem Commun (Camb) ; 46(4): 586-8, 2010 Jan 28.
Article in English | MEDLINE | ID: mdl-20062870

ABSTRACT

An amino-calix[6]arene was combined with sugar-based surfactants, using an acid-base reaction, to obtain an original catanionic association. Physicochemical studies showed the spontaneous formation of stable vesicles in aqueous solution.


Subject(s)
Calixarenes/chemistry , Surface-Active Agents/chemistry , Amines/chemistry , Carbohydrates/chemistry , Models, Molecular , Molecular Structure
20.
Bioorg Med Chem ; 18(1): 242-8, 2010 Jan 01.
Article in English | MEDLINE | ID: mdl-19942442

ABSTRACT

The synthesis and characterization of a new series of catanionic multivalent analogs of GalCer is described. These systems are based on phosphonic acid terminated dendrimers and N-hexadecylamino lactitol moieties. Despite important structural differences that affect the dendrimers' outer-shell, these supramolecular assemblies showed a fairly comparable anti-HIV-1 activity. All compounds have submicromolar IC(50) in a cell-based HIV-infection model but also a high general cytotoxicity.


Subject(s)
Anti-HIV Agents/chemistry , Anti-HIV Agents/pharmacology , Galactosylceramides/chemistry , Galactosylceramides/pharmacology , HIV Infections/drug therapy , HIV-1/drug effects , Anti-HIV Agents/chemical synthesis , Cell Line , Cell Survival , Dendrimers/chemical synthesis , Dendrimers/chemistry , Dendrimers/pharmacology , Galactosylceramides/chemical synthesis , Humans , Microbial Sensitivity Tests , Organophosphonates/chemical synthesis , Organophosphonates/chemistry , Organophosphonates/pharmacology
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