ABSTRACT
OBJECTIVE: This study aimed to analyze in vitro cytotoxicity to cultured 3T3 fibroblasts and in vivo inflammatory reaction in rats by calcium hypochlorite (Ca(OCl)2) solutions compared with sodium hypochlorite (NaOCl) solutions. DESIGN: Cultured 3T3 fibroblasts were exposed to different concentrations of (Ca(OCl)2) and NaOCl solutions, and a scratch assay was performed. The viability rate was analyzed with trypan blue assay. Both solutions of 1% and 2.5% concentrations were injected into the subcutaneous tissue of 18 male Wistar rats aged 18 weeks. The inflammatory tissue reaction was evaluated at 2h, 24h, and 14days after the injections. The samples were qualitatively analyzed using a light microscope. Statistical analysis was performed with ANOVA and Tukey post hoc tests for in vitro assays and Kruskal-Wallis and Dunn post hoc tests for in vivo assays (α=0.05). RESULTS: In the scratch assay, Ca(OCl)2 showed no significant difference compared with the control group (culture medium) at 24h (p<0.05). Solutions of 0.0075% and 0.005% NaOCl and Ca(OCl)2 concentrations presented similar results compared with those in the positive control group (hydrogen peroxide) (p>0.05) in the trypan blue assay. In the in vivo assay, 1% Ca(OCl)2 group showed a significant decrease in neutrophils at 2h and 24h (p=0.041) and 2h and 14days (p=0.017). There was no statistically significant difference for lymphocyte/plasmocyte and macrophage counts among the different concentration groups. CONCLUSIONS: Ca(OCl)2 showed favorable results of viability and induced a low-level inflammatory response. Ca(OCl)2 presented acceptable cytotoxicity and biocompatibility as an irrigant solution.
Subject(s)
Calcium Compounds/pharmacology , Cell Movement/drug effects , Cell Survival/drug effects , Fibroblasts/drug effects , Root Canal Irrigants/pharmacology , Animals , Biocompatible Materials/pharmacology , Cells, Cultured , Male , Rats , Rats, Wistar , Sodium Hypochlorite/pharmacologyABSTRACT
Introduction: The study aimed to explore the profile of articles published on pulp revascularization by a bibliometric analysis. Material and methods: A search was conducted on the PubMed database and studies were independently categorized according to: (i) year of publication; (ii) country where the study was conducted; (iii) study design; (iv) main topic addressed; (v) main conclusion of the article; and (vi) clinical application/other results. Findings were reported descriptively. There was not restriction regarding to initial date and the established final date was December 2014. Results: 133 studies were found; but only 86 articles were included. The most of them was published recently (23.2% in 2014) and were conducted in 24 different countries. The most frequent study design was case report (37.2%), followed by narrative reviews (16.2%). The main topic addressed was the radiographic outcomes (38.3%) and tissue engineering (19.7%). Some of the articles point the clinical application of their results. Conclusion: Most of the publications highlight that the disinfection of the root canal (and the stimulation of residual stem cells) would induce formation of new hard tissue on the dentin wall and continue root development in length, improving the tooth survival. The number of publications on pulp revascularization has increased recently, but the majority of articles published are studies with low levels of evidence.
