ABSTRACT
Potentiometric and spectrophotometric titrations, isoelectric focusing and amino acid analyses, have been made on the hemocyanin from Jasus edwardsii. Counts of acidic neutral and alkaline groups were made from the titrations, enabling comparisons to be made with the amino acid analysis. Thermodynamic analysis of the data indicated that changes in the native protein structure took place at pH 4.0, 8.4 and 10.7. These observations are discussed in terms of dissociation, shifts in pK and conformational changes in the protein.
Subject(s)
Hemocyanins , Amino Acids/analysis , Animals , Guanidines , Hydrogen-Ion Concentration , Nephropidae , Potentiometry , Spectrophotometry, UltravioletABSTRACT
Digoxin levels in post-mortem specimens are reported for two unusual cases. The blood level of digoxin in one of these cases was five times greater than any previously reported. The other case involved specimens from an embalmed body.
