Chemical enhancement of footwear impressions in urine on fabric.

A range of chemical techniques were utilised for the enhancement of footwear impressions deposited on a variety of fabric types of different colours with urine as a contaminant. A semi-automated stamping device was used to deliver test impressions at a set force to minimise the variability between impressions; multiple impressions were produced and enhanced by each reagent to determine the repeatability of the enhancement. Urine samples from different donors were analysed using a spectrofluorophotometer revealing differences between individuals. Results indicated that the enhancement of footwear impressions in urine was possible using amino acid staining techniques whereas protein stains failed to achieve successful enhancement.

Spectroscopic imaging of latent fingermarks collected with the aid of a gelatin tape.

This work introduces a new and nondestructive methodology for the collection and chemical identification of latent fingermarks. The main challenges of this work were (a) to find an appropriate medium to lift fingermarks from various surfaces and (b) to develop an analytical approach for the identification of small quantities of sample while avoiding spectroscopic interference from the lifting media. Two different lifting media were evaluated and analyzed by ATR-FT-IR spectroscopic imaging, which affords inherent chemical specificity with rapid acquisition of data. This is the first time that chemical images of latent fingermarks collected with gel lifters from different surfaces have been obtained. Spatially resolved chemical images from different depths within the same sample were obtained using ATR-FT-IR imaging with a variable angle ATR accessory to minimize interference from the substrate. The possibility of obtaining, through the developed methodology, three-dimensional depth profiles of surface contaminants collected with the lifting gel shows great potential for the investigation of samples for forensic interest.