ABSTRACT
Metabolism of 5 microM benzylamine (BZ) by rat aortic cultured smooth muscle cells (SMC) is inhibited almost completely by 10(-3) M semicarbazide and 10(-6) M propargylamine, but is little affected by 10(-4) M and 10(-3) M pargyline and clorgyline, indicating BZ metabolism predominantly by the semicarbazide-sensitive amine oxidase (SSAO) previously characterized in rat aortic homogenates. Km values of 7-9 microM for BZ metabolism by SSAO in SMC cultures, indicate similar, if not identical properties, to the enzyme in the parent blood vessel.
Subject(s)
Amine Oxidase (Copper-Containing)/metabolism , Muscle, Smooth, Vascular/enzymology , Amine Oxidase (Copper-Containing)/antagonists & inhibitors , Animals , Aorta, Thoracic/cytology , Aorta, Thoracic/enzymology , Benzylamines/metabolism , Cells, Cultured , Clorgyline/pharmacology , In Vitro Techniques , Kinetics , Male , Monoamine Oxidase Inhibitors/pharmacology , Muscle, Smooth, Vascular/cytology , Pargyline/pharmacology , RatsSubject(s)
Hematology/history , Bibliographies as Topic , History, 20th Century , Poland , Schools, Medical/historySubject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Interferon Type I/administration & dosage , Multiple Myeloma/drug therapy , Clinical Trials as Topic , Cyclophosphamide/administration & dosage , Humans , Melphalan/administration & dosage , Prednisone/administration & dosage , Recombinant Proteins , Vincristine/administration & dosageABSTRACT
During the last 2 years, we have treated 14 hairy cell leukemia (HCL) patients with human recombinant interferon-2 alpha (Boehringer Ingelheim). The above group consisted of eight nonsplenectomized and six previously splenectomized progressive HCL patients. The patients received daily doses of 5 x 10(6) units of IFN for 3 months and two doses per week for the next 3 months thereafter by i.m. route. The therapy resulted in the complete (nine cases, = 64.3%) or partial (two cases = 14.3%) clinical and hematological remission (response rate 78.6%), with either disappearance or marked reduction in circulating and bone marrow hairy cells, decreased spleen size, and recovery of normal hemopoiesis. Apart from a transient flulike syndrome during the first 2 weeks of therapy, no other side effects were observed.
Subject(s)
Interferon Type I/therapeutic use , Interferon-alpha/therapeutic use , Leukemia, Hairy Cell/therapy , Adult , Erythrocyte Count , Female , Humans , Interferon alpha-2 , Male , Microscopy, Electron , Middle Aged , Platelet Count , Receptors, Antigen, B-Cell/analysis , Recombinant Proteins , SplenectomyABSTRACT
Activity of acid phosphatase, beta-glucuronidase and N-acetyl-beta-D-glucosaminidase in peripheral blood neutrophils of 30 women with breast cancer has been studied by means of semiquantitative cytochemical methods. In comparison to 20 healthy women the patients showed an increased activity of acid phosphatase and beta-glucuronidase whereas that of N-acetyl-beta-D-glucosaminidase was significantly lowered. It has been suggested that observed intracellular enzymatic deficiency is of importance with regard to lowered antitumor activity of neutrophils. Analogous deficiency of N-acetyl-beta-D-glucosaminidase within the neutrophils has previously been observed by the authors in women with malignant tumors of reproductive organs.
Subject(s)
Breast Neoplasms/blood , Neutrophils/enzymology , Acetylglucosaminidase/metabolism , Acid Phosphatase/metabolism , Adult , Female , Glucuronidase/metabolism , Humans , Middle AgedABSTRACT
In 55 patients with diabetes mellitus metabolically balanced the activity and intracellular localization of acid phosphatase has been estimated by means of semiquantitative method of Barka and Anderson [3] in peripheral blood lymphocytes. It has been shown that patients in question, both insulin-dependent and those with overweight, in comparison to control group, exhibit more than twofold lower numbers of lymphocytes having intact enzyme positive lysosomes and more than twofold higher numbers of that cells with damaged lysosomes and extralysosomal localisation of the enzyme. According to authors suggestion the above changes may be associated with disturbed lymphocyte functions in the patients with diabetes mellitus.
