ABSTRACT
Bacterial vaginosis (BV) is characterised by depletion of the normal Lactobacillus spp. and overgrowth of commensal anaerobic bacteria. We investigated the composition of vaginal microbiota and their association with BV in women of reproductive age. Vaginal samples from 1197 women were analysed, whereby n=451 patients had normal flora and n=614 were diagnosed with BV, the remaining patients were diagnosed with having either intermediate flora (n=42) or dysbacteriosis (n=90). The reported results show that pathogens are associated with BV. This knowledge will further expand our understanding of events leading to BV, which may lead to more effective prevention and treatment strategies.
Subject(s)
Candida/isolation & purification , Gastrointestinal Tract/microbiology , Gram-Positive Bacteria/isolation & purification , Sexually Transmitted Diseases/microbiology , Vaginosis, Bacterial/microbiology , Adolescent , Adult , Candida/classification , Candida/genetics , Candida/growth & development , Cross-Sectional Studies , Female , Gram-Positive Bacteria/classification , Gram-Positive Bacteria/genetics , Gram-Positive Bacteria/growth & development , Humans , Microbiota , Vagina/microbiology , Young AdultABSTRACT
A 53-year-old homosexual man presented at his general practitioner (GP) practice with a suspicion of sexually transmitted infection. Initial NAAT screening was performed for Chlamydia trachomatis and Neisseria gonorrhoeae. The patient was positive for Neisseria gonorrhoeae both for his urine and rectal sample. The subsequent confirmation test for Neisseria gonorrhoeae by a second laboratory was only confirmed for the urine sample and the rectal sample was negative. We report a case of a potential false-negative diagnosis of Neisseria gonorrhoeae due to mutations of DNA sequence in the probe region of opa-MGB assay of the rectal sample. The patient did not suffer any discomfort as diagnosis of Neisseria gonorrhoeae in his urine sample had already led to treatment by prescribing the patient with Ceftriaxone 500 mg IV dissolved in 1 ml lidocaine 2% and 4 mL saline. The patient also received a prescription for Azithromycin (2x500 mg).
Subject(s)
Bacterial Outer Membrane Proteins/genetics , Gonorrhea/diagnosis , Mutation , Neisseria gonorrhoeae/isolation & purification , Rectum/microbiology , Anti-Bacterial Agents/administration & dosage , Chlamydia Infections , Diagnostic Errors , False Positive Reactions , Gonorrhea/drug therapy , Gonorrhea/microbiology , Humans , Male , Middle Aged , Multiplex Polymerase Chain Reaction/methods , Neisseria gonorrhoeae/geneticsABSTRACT
We have developed and validated a multiplex-PCR method for detection of dermatophyte spp., Candida albicans and parapsilosis for routine diagnostics. Our m-PCR showed excellent concordance with culture results in 475 clinical samples. Through the rapid diagnosis by our m-PCR, clinicians are able to initiate adequate antimycotic therapy much earlier.
Subject(s)
Candida albicans/isolation & purification , Candida/isolation & purification , Candidiasis, Cutaneous/diagnosis , Multiplex Polymerase Chain Reaction/methods , Arthrodermataceae/genetics , Arthrodermataceae/isolation & purification , Candida/genetics , Candida albicans/genetics , DNA Primers , DNA, Fungal/isolation & purification , Hair/microbiology , High-Throughput Nucleotide Sequencing , Humans , Nails/microbiology , Sensitivity and Specificity , Sequence Alignment , SkinABSTRACT
The purpose of this study is to introduce a high-throughput system, Aurora FLOW, for the simultaneous detection of 3 clinically relevant pathogens of sexually transmitted infections. Comparative evaluation with other systems revealed an overall concordance of 97.9% for Chlamydia trachomatis and comparable performance for Neisseria gonorrhoeae and Trichomonas vaginalis.
