ABSTRACT
Peroxidase-labelled Helix pomatia A hemagglutinin (HPH) was used as a T-cell marker for neuraminidase-treated human lymphocytes from blood or cerebrospinal fluid (CSF). Lymphocytes from the blood of 22 patients with noninflammatory diseases of the central nervous system and from the CSF of 16 patients with noninfectious diseases and 29 patients suffering from meningitis or meningoencephalitis were studied. Most HPH-binding cells were found in normal CSF. The variance in the number of reactive lymphocytes was higher in the CSF from patients with inflammatory diseases than in the other types of samples.
Subject(s)
Blood Cells/cytology , Cerebrospinal Fluid/cytology , Lectins/metabolism , T-Lymphocytes , Cell Separation , Central Nervous System Diseases/blood , Central Nervous System Diseases/cerebrospinal fluid , Humans , Leukocyte Count , Receptors, Mitogen/metabolismABSTRACT
In 208 patients, the IgG-synthesizing lymphocytes of the CSF were demonstrated with immunocytochemical methods. These cells were found in 59 of 111 patients with multiple sclerosis. Their frequency of appearance was dependent on the duration of the disease and its clinical severity. A comparative laser-nephelometric examination of serum and CSF, together with CSF cell diagnosis, demonstrated that IgG-synthesizing lymphocytes were present in 12% of the patients without simultaneous local IgG production within the CNS. These cases showed mostly very short clinical courses. IgG-synthesizing lymphocytes were absent from the CSF of 97 patients with non-inflammatory diseases of the nervous system.
Subject(s)
Immunoglobulin G/biosynthesis , Lymphocytes/metabolism , Multiple Sclerosis/cerebrospinal fluid , Nervous System Diseases/cerebrospinal fluid , Adolescent , Adult , Female , Histocytochemistry , Humans , Immunochemistry , Immunoglobulin G/cerebrospinal fluid , Male , Middle AgedABSTRACT
OKT8-binding lymphocytes, which consist mainly of suppressor-cytotoxic T cells, were demonstrated in blood and CSF by an immunochemical method. In patients with non-inflammatory diseases, the mean value was 18% in blood and 19% in CSF. In acute viral infections of the CNS, on the other hand, the percentage was significantly lower in the initial days of the disease but then increased up to the 5th week. Eleven patients suffering from multiple sclerosis had similar percentages of OKT8-binding cells, whether or not they had acute symptoms.
