ABSTRACT
The effects of the small doses of the preparation Gistochrome, containing natural polyhydroxynaphtoquinoine echinochrom A from flat sea urchin Scaphechinus mirabilis, on blood biochemical parameters have been studied in patients with cardiovascular diseases. Gistochrome administration influenced the LPO-antioxidant protection system, indicating reinforcement of antioxidant protection mechanisms. Gistochrome modulated the immune status and the plasma cytokine profile. Thus, Gistochrome may be recommended as means of additional therapy for patients with cardiovascular diseases for correcting the metabolic, immunological and redox impairments.
Subject(s)
Antioxidants/therapeutic use , Atherosclerosis/blood , Hypertension/blood , Immunologic Factors/therapeutic use , Myocardial Ischemia/blood , Naphthoquinones/therapeutic use , Animals , Antigens, CD/metabolism , Antioxidants/isolation & purification , Atherosclerosis/drug therapy , Atherosclerosis/physiopathology , Blood Cell Count , Case-Control Studies , Cholesterol, HDL/blood , Cholesterol, LDL/blood , Cholesterol, VLDL/blood , Cytokines/metabolism , Humans , Hypertension/drug therapy , Hypertension/physiopathology , Immunologic Factors/isolation & purification , Injections, Intramuscular , Lipid Peroxidation/drug effects , Malondialdehyde/blood , Middle Aged , Myocardial Ischemia/drug therapy , Myocardial Ischemia/physiopathology , Naphthoquinones/isolation & purification , Oxidation-Reduction/drug effects , Sea Urchins/chemistry , Triglycerides/bloodABSTRACT
Development of exudative skin necrosis in female rats of Vistar line was caused by Selye method (with help of subcutaneons injections of sodium chlorate). The number of rats which got skin necrosis was bigger than when animals were fed on food containing butter or sardine fat, at that in the second case bigger than first one. The necrosis area in rats, which were fed on different rations, was the some.
Subject(s)
Fatty Acids, Omega-6/administration & dosage , Skin Diseases/prevention & control , Animals , Exudates and Transudates , Fatty Acids, Unsaturated/administration & dosage , Female , Necrosis/chemically induced , Necrosis/prevention & control , Rats , Rats, Wistar , Skin Diseases/chemically induced , Sodium Chloride/toxicityABSTRACT
We obtained a new food preservative from marine fish lipids possessing pronounced activity in relation to bacteria and microscopic fungi. The effects of this preparation on enzymes of microorganisms and muscle tissue of marine hydrobionts were studied. In vitro the preparation irreversibly inhibited acid and alkaline proteases and proteolytic and lipolytic enzymes of microorganisms and reduced enzyme activity in fish muscle tissue. The inhibitory effect of this preparation on enzymes contributes to stabilization of hydrolytic processes in meat of hydrobionts and suppresses microorganism growth in storage.
Subject(s)
Anti-Infective Agents/pharmacology , Bacteria/drug effects , Enzyme Inhibitors/pharmacology , Lipids/chemistry , Mitosporic Fungi/drug effects , Muscle, Skeletal/drug effects , Animals , Anti-Infective Agents/chemistry , Dose-Response Relationship, Drug , Endopeptidases/analysis , Enzyme Inhibitors/chemistry , Fishes/metabolism , Food Preservatives/chemistry , Food Preservatives/pharmacology , Gelatinases/analysis , Lipid Metabolism , Metalloendopeptidases/analysis , Muscle, Skeletal/enzymology , Oceans and Seas , Peptide Hydrolases/analysis , Phospholipases/analysisABSTRACT
A mixture of water-soluble oxidation products of Sardinops melanosticta sardine oil was found to contain (2E)-4-hydroxy-2-nonenal. This was isolated by column chromatography on silica gel and reversed-phase HPLC. Its structure was elucidated by physicochemical methods. The activity of (2E)-4-hydroxy-2-nonenal against test cultures of Escherichia coli, Staphylococcus aureus, and Bacillus subtilis was about 20% of the total antimicrobial activity of the preparation.
