[Results of treatment of acute leukemia in children using intensive therapy programs]. / Rezul'taty lecheniia ostrykh leikozov u detei s ispol'zovaniem programm intensivnoi terapii.

The authors report the results of the treatment according to the programs BFM-ALL-90 and BFM-AML-83 and 87. A total of 110 children with acute lymphoblastic leukemia (ALL) and 35 with acute myeloblastic leukemia (AML) were treated with remission rate 94.5% and 74.5%, respectively. Under programmed treatment of ALL the recurrences occurred in 12.2% against 46% of the cases in nonprogrammed treatment. 2-year survival made up 75% and 47.3%, respectively. Among AML cases there were frequently prognostically unfavorable ones and patients with neuroleukemia this dictating the necessity of the treatment intensification and irradiation of the skull in AML. Improvement of adjuvant therapy is a must in advance of acute leukemia treatment.

[Lymphocyte subpopulations in the bone marrow of children]. / Subpopuliatsii limfotsitov kostnogo mozga u detei.

Blood lymphocyte subpopulations, and bone marrow aspiration and biopsy materials were comparatively investigated in 10 children without hematologic or infectious diseases (the children had chest deformations and diaphragmatic hernias). Lymphocyte typing was conducted by the method of continuous cytofluorometry in a complete leucocyte suspension with the use of monoclonal antibodies. The results of the study have evidenced that the content of lymphocytes carrying T-cell markers in the aspiration material was twofold lower, and in the biopsy material fourfold lower than in the peripheral blood. The number of immature T-lymphocytes in the bone marrow is higher than their content in the peripheral blood. A total of 40% of aspiration cells and 60% of biopsy cells present in the lymphocytic window, do not carry lymphocytic markers. CD8+-lymphocytes prevail in the bone marrow.

[Immunohematologic characteristics and course of chronic neutropenia in children with severe decrease of granulocyte maturation]. / Immunogematologicheskaia kharakteristika i techenie khronicheskikh neitropenii u detei s vysokim obryvom sozrevaniia granulotsitov.

The clinical course and some immunohematologic parameters of the blood and bone marrow were investigated in chronic neutropenia children with a high break of granulocyte maturation. Heterogeneity of etiopathogenetic mechanisms of the disease has been recorded in this group of children.

[Dynamics of initial L-cell adhesion. II. Competition of albumin and serum activating factor]. / Dinamika nachal'nogo prikrepleniia L'kletok. II. Konkurentsiia al'bumina i aktiviruiushchego faktora syvorotki.

The initial attachment process of L cells is studied by the combined action of serum and albumin. Both the substances are added jointly in the Eagle medium or one of them was adsorbed on the substrate previously. The results show that there are two factors in the serum: one depressing the cell attachment, like albumin, and the other being just opposite. The simple kinetical competition model is suggested to describe the experimental dependence of final level of attachment on the concentrations of serum and albumin. The examination of the thermal resistance of the serum factor is made; the previous heating to 60-100 degrees C increases the depressing effect of serum and albumin.

[Dynamics of initial L-cell adhesion. I. The effect of serum and albumin]. / Dinamika nachal'nogo prikrepleniia L-kletok. I. Vliianie syvorotki i al'bumina.

The adhesion of neoplastic mouse fibroblasts of line L was investigated during the formation of initial cell-substrate contact after the settling of spherical cells from suspension. nu (t), part of cell population which remained attached to the substrate after the impulse action of detaching force in the moment t, is used as a quantitative measure of the initial attachment process. The effects of the detaching force value and of the experimental medium composition on the main parameters of sigmoidal function nu (t) are studied. It is shown that 1) with the change of the detachment force three main parameters of function nu (t) are changed; 2) with the increase of the concentration of serum added in the Eagle medium the cell attachment becomes monotonely weaker and slower; albumin has the similar effect.

[Investigation of initial stage of fibroblast attachment by means of interference reflection microscopy]. / Izuchenie nachal'noi stadii prikrepleniia fibroblastov metodom otrazhatel'noi interferentsionnoi mikroskopii.

The initial cell--substrate contach, arising before the beginning of fibroblast spreading is formed from the multitude of discrete microcontacts (MC). The number of these MC increases monotonously and at room temperature 20 degrees C reaches the constant maximum value three hours after the cell settling from the suspension. An increase of MC number is accompanied by two phenomena: 1) expansion of the MC zone from the cell pole to periphery; 2) the following formation of the central continuous contact as a consequence of the fusion of the adjacent discrete MC. The zone of continuous contact expands also from the pole to periphery. The quantitative characteristics of this process are presented and the model of initial attachment as the successive attachments of the cell surface projections are discussed.

[Kinematics of fibroblast spreading. II. Individual locomotor activity]. / Kinematika rasplastyvaniia fibroblastov. II. Individual'naia lokomotornaia aktivnost'.

An analysis of area changes of normal mouse fibroblasts during their spreading is made with time intervals of 5-50 s. A mean increase of the area takes place simultaneously with area fluctuations of various rhythms, which result from accidental undulations of the lamella edge. The fluctuations are also the same during intermediate temporary stopping of cell spreading. The central cell region--endoplasm--is stretched passively by centrifugal lamella forces and is an active antagonist of forces in squeeze processes. A description is made of the lamella edge undulations like the phase states change of protraction and retraction, and it is shown by two-dimensional phase diagrams that edge-activity has a zone organisation. These zones can migrate upon the cell perimeter as a wave in stohastic excitable medium.

[Cinematics of fibroblast spreading. I. Analysis of a population]. / Kinematika rasplastyvaniia fibroblastov. I. Analiz populiatsii.

Normal mouse fibroblasts were studied by means of time-lapse microcinematography under phase-contrast microscope. For 31 cells the area measurements during spreading were made with the intervals of 2.5--5 minutes. The following results have been obtained. The maximal area of spreaded cell is 3--4 times greater than the surface area of the sphere with the spherical cell radius. There is a tendency of the cell area to increase linearly in the middle, that is in a regime of a constant middle rate. Most cells spread either in one regime, or in two, when a more rapid regime is followed by a shower one. The change of the spreading regime is a result of the control of mean rate of spreading.

[Kinetics of fibroblast spreading]. / Kinetika rasplastyvaniia fibroblastov

For normal mouse fibroblasts four stages of their spreading in vitro are distinguished: the spherical cell stage; the stage of early appearance of processes, Lamellae formation; the stage of maximal cell spreading. Measurements of the duration of every stage were performed by means of time-lapse microcinematography under phase-contrast microscope. It is proved that the first and second stages are elementary ones of Puasson's kinetics and the following ones are complex stages of the same kinetic model. The mean time at these stages is respectively: 25+/-3.2, 12.5+/-1.5; 123+/-8; 162+/-25 minutes.