[Disruption of nerve tissue cholesterol metabolism in the case of an excess of vitamin A in the growing body]. / Narushenie obmena kholesterina nervnoi tkani pri izbytke vitamina A v rastushchem organizme.

Subcellular distribution and the rate of labelled precursors incorporation into fractions of cholesterol esters were studied in brain and spinal cord of growing rats consuming overdoses of vitamin A. Per os administration of retinyl acetate during 10-16 days of postnatal period at a dose of 1,000 IU resulted in intracellular imbalance of cholesterol content and in distinct increase of cholesterol esters in all the subcellular fractions studied. A decrease of 2-(14)C- acetate incorporation into cholesterol esters of nervous tissue caused by vitamin A overloading was also detected. Analysis of gas chromatograms showed an increase in content of palmitic acid as well as a decrease of unsaturated acids in fractions of cholesterol esters of these animals brain tissues. The impairments of cholesterol metabolism found in nervous tissue of growing body under conditions of retinol overloading may be responsible for deterioration of myelinization.

[Fatty acid composition of separate fractions of lipids from tumor tissue in breast cancer]. / Zhironokislotnyi sostav otdel'nykh fraktsii lipidov opukholevoi tkani pri rake molochnoi zhelezy.

The distribution of major fatty acids in neutral lipids and phospholipids was studied in breast tumor tissue. The most pronounced differences in the content of individual fatty acids were found in the fractions of diacylglycerol and phosphatidylethanolamine, which mainly applies to unsaturated acids. Possible impairments of transacetylation mechanisms in carcinogenesis are discussed.

[The action of dipyridamole on megakaryocytopoiesis in regenerating and stationary bone marrow cell populations]. / Deistvie dipiridamola na megakariotsitopoéz v regeneriruiushchei i statsionarnoi populiatsiiakh kletok kostnogo mozga.

The effect of dipyridamole on megakaryocytopoiesis in regenerating and stationary populations of mouse bone marrow cells has been studied by heterotopic transplantation of the bone marrow using histological, electron microscopic and biochemical techniques. It is shown that drug administration induced destruction of megakaryocytes. In megakaryocytic cytoplasm giant lipid granules were found whose growth and number increase resulted in megakaryocytes kill. Gas-liquid chromatography was used to evaluate the effect of dipyridamole on distribution of lipid fatty acids of the stationary and regenerating populations of the bone marrow cells. A marked increase of the percentage of docosahexaenoic acid was found in lipids of the stationary population. Chronic dipyridamole administration caused an increase of percentage of myristic, palmitic oleic acids, and decrease of percentage of arachidonic and eicosapentaenoic acids in lipids of regenerating bone marrow cells population.

[Effect of thyrotoxicosis on lipid fatty acid composition in various subcellular fractions of the rat brain]. / Deistvie tireotoksikoza na zhirnokislotnyi sostav lipidov razlichnykhsubkletochnykh fraktsii golovnogo mozga krys.

Thyrotoxicosis, which developed after subcutaneous administration of L-thyroxin at a dose of 400 micrograms/100 g of body mass, was accompanied by distinct alteration of fatty acid composition in lipids of adult rat brain myelin, synaptosomes, mitochondria and microsomes. The most significant alterations were detected in synaptosomes and mitochondria. Under conditions of thyrotoxicosis the ratio of polyunsaturated docosahexaenic and docosatetraenic acids was decreased in synaptosome lipids, whereas the content of palmitic, oleic and neuronic acids was increased. The ratio of unsaturated arachidonic and docosahexaenic fatty acids was increased but content of palmitic and linoleic acids was decreased in mitochondrial lipids.

[Fatty acid composition of total lipids and phospholipids of membrane preparations of transport ATPases]. / Zhirnokislotnyi sostav summarnykh lipidov i fosfolipidov membrannykh preparatov transportnykh ATPaz.

The fatty acid composition of total lipids and phospholipids of duck salt gland Na,K-ATPase (outer plasma membrane) and of rabbit skeletal muscle Ca-ATPase (intracellular membrane) was investigated. The bulk of Na,K-ATPase fatty acids is represented by palmitic (16:0), oleic (18:1), stearic (18:0) and arachidonic (20:4) acids. The duck salt gland is characterized by rather a high content of unsaturated fatty acids, especially of arachidonic acid. The unsaturation index of total-lipid fatty acids increases during purification of these preparations in the following order: homogenate greater than microsomal fraction greater than purified enzyme. The fatty acid composition of Na,K-ATPase total lipids and phospholipids reveals certain differences. Phospholipids contain more stearic and liholeic (18:2) acids than total lipids, but the level of arachidonic acid in them is twice as low. Besides, phospholipids were found to contain polyunsaturated docosohexaenic (22:6) acid. The bulk of fatty acids of rabbit skeletal muscle Ca-ATPase total lipids and phospholipids is represented by 16:0, 18:0, 18:1 and 18:2 acids. The content of polyunsaturated fatty acids in this preparation is much lower than in duck salt gland Na,K-ATPase. The fatty acid composition of total lipids and phospholipids in rabbit skeletal muscle Ca-ATPase differ insignificantly. The differences in the fatty acid composition of membrane preparations under study is conditioned mainly by the fractional composition of their lipids.