[The effect of Opisthorchis on the manifestations of herpesvirus type 2 in an experiment and their possible participation in the mechanism of the occurrence of primary liver cancer]. / Vliianie opistorkhisov na proiavleniia virusov gerpesa vtorogo tipa v éksperimente i ikh vozmozhnoe uchastie v mekhanizme vozniknoveniia pervichnogo raka pecheni.

The impact of opisthorchiasis on the manifestations of herpes simplex viruses type 2 (HSV-2) was studied. The parasites were established to tend to cause the body's hypersensitivity to HSV-2, particularly after repeated invasions and in summer, the process being characterized by urogenital and bile duct lesions coupled with high proliferation rate in their epithelium. Opisthorchiasis became a reservoir and an additional source of viral infection. There were also a definite damage to their reproductive system. The possible involvement of HSV-2 in the development of primary liver carcinoma in opisthorchiasis is discussed in the paper.

[A comparison of the results of analyzing the clinical data from gynecological, ophthalmological and dental patients using a set of rapid methods for the diagnosis of herpesvirus infection]. / Sopostavlenie rezul'tatov analiza klinicheskikh materialov ginekologicheskogo, oftal'mologicheskogo i stomatologicheskogo profilei naborom ékspress-metodov diagnostiki gerpesvirusnoi infektsii.

Analysis of biological specimens from gynecological, ophthalmological, and stomatological patients was carried out using current express methods for diagnosis of herpes virus infection developed by the authors: enzyme immunoassay (EIA), time-resolved fluoroimmunoassay (tr-FIA), molecular hybridization and conventional immunofluorescence technique. Monoclonal antibodies (MCA) generated by us with epitope specificity for gpD HSV-2 were used in EIA and tr-FIA. F(ab')2-fragments of MCA used as sorbents and for preparation of conjugates were found to be by 250% more sensitive than intact IgG used for similar purposes. The employment of EIA based on MCA F(ab')2-fragments contributed to detection of 0.2-0.6 ng of virus-specific protein in a specimen. Comparison of the results of analysis of clinical specimens of broad spectrum (mucous secreta from the cervical canal of the uterus and posterior vault of the vagina, saliva, lacrimal fluid) by the above express methods for diagnosis of herpes virus infection revealed a high percentage of positive findings, none of the methods providing 100% diagnosis. The use of a set of methods makes the resultant information more objective. Therefore, in future the data obtained by a number of current express methods for herpes diagnosis should be compared.

[The immunotherapy of herpetic stomatitis in children]. / Immunoterapiia gerpeticheskogo stomatita u detei.

Immunoglobulin with a high titer of antiherpetic antibodies (1:640 to 1:1280) was used for the treatment of children with acute and recurrent herpetic stomatitis. The agent was injected intramuscularly, 2 to 4 injections, depending on the disease severity. The results evidence a favorable effect of the drug on the clinical and immunologic parameters of patients suffering from the acute condition and permit a conclusion that this immunoglobulin prevented the development of recurrent forms in the children with the acute disease.

[Development of a solid-phase immunoenzyme method for determining the antibody level in the blood sera of persons vaccinated with influenza vaccine]. / Razrabotka tverdofaznogo immunofermentnogo metoda dlia opredeleniia urovnia antitel v syvorotkakh krovi liudei, vaktsinirovannykh grippoznoi vaktsinoi.

To control the effectiveness of vaccination against influenza, the optimum conditions for making the enzyme-linked immunosorbent assay (ELISA) with a view to determine the level of anti-influenza antibodies in human blood sera have been established. The kinetics of influenza virus adsorption in the wells of ELISA polystyrene plates and the kinetics of the interaction between the immobilized antigen and species-specific peroxidase-labeled antibodies have been studied. The method has been shown to be more sensitive than the hemagglutination inhibition test in the determination of seroconversion in persons immunized with influenza vaccine.

[Immunoenzyme analysis. Quantitative determination of antibodies to the influenza virus]. / Immunofermentnyi analiz. Usloviia kolichestvennogo opredeleniia antitel k virusu grippa.

Solid-phase immunoenzymatic procedure for quantitative estimation of antibodies to virus of influenza A was developed. A relationship was found between the rate of influenza virus adsorption on polystyrene and concentration of the cells, duration of the adsorption, temperature and the nature of buffer systems. Optimal conditions were developed for binding of the virus with polystyrene, immune sera and the conjugate IgG-peroxidase. The suitable procedure is described for immunoenzymatic analysis in serologic diagnosis of influenza.

[Immune ascitic fluids in rats to the Sindbis virus]. / Immunye astsiticheskie zhidkosti krys k virusu Sindbis.

Data are presented concerning preparation of universal immune ascitic fluid (IAF) to Sindbis virus possessing a high activity and specificity in different serological tests: CFT, HI, NT, AGDPT, and direct immunofluorescence test. The advantages of rat IAF over the serum of these animals consists in a higher specific activity, lower amount of nonspecific hemagglutination inhibitors and waste protein substances. Rat IAF may be used for production of highly specific diagnostic preparations.

[Obtaining group-specific fluorescent immunoglobulin for diagnosis of adenovirus infection]. / Poluchenie gruppospetsificheskogo fluorestsiruiushchego immunoglobulina dlia diagnostiki adenovirusnoi infektsii.

The authors present the results of a comparative study of several schemes of rabbit immunization with mixtures consisting of 4 and 7 (mixture No. 1) nad 2, 4, and 7 (mixture No. 2) adenovirus types for the purpose of obtaining group-specific fluorescent immunoglobulins. Since a direct correlative dependence of the activity of fluorescent immunoglobulins on the antibody level to the adenoviruses of homologous type was revealed in the immune sera a combined scheme of mixture No. 2 administration was elaborated for the immunization of animals.