Subject(s)
Chromosomes, Human/genetics , Chromosomes/genetics , Pan troglodytes/genetics , Amino Acid Substitution , Animals , Base Sequence , DNA/genetics , Evolution, Molecular , Gene Expression , Genome , Genome, Human , Humans , Molecular Sequence Data , Promoter Regions, Genetic , Proteins/genetics , Receptor, Interferon alpha-beta , Receptors, Interferon/genetics , Sequence Homology, Nucleic Acid , Species SpecificityABSTRACT
We converted the small homodimeric four-helix bundle repressor of primer protein (Rop) into a monomeric four-helix bundle by introduction of connecting loops. Both left- and right-handed four-helix bundles were produced. The left-handed bundles were more stable and were used to introduce biologically interesting peptides in one of the loops.
Subject(s)
Bacterial Proteins/chemistry , RNA-Binding Proteins/chemistry , Amino Acid Sequence , Chromatography, Gel , Escherichia coli/metabolism , Models, Molecular , Molecular Sequence Data , Peptides/chemistry , Protein Conformation , Protein Folding , Protein Structure, SecondaryABSTRACT
Listeria monocytogenes is a food-borne pathogen with a high mortality rate that has also emerged as a paradigm for intracellular parasitism. We present and compare the genome sequences of L. monocytogenes (2,944,528 base pairs) and a nonpathogenic species, L. innocua (3,011,209 base pairs). We found a large number of predicted genes encoding surface and secreted proteins, transporters, and transcriptional regulators, consistent with the ability of both species to adapt to diverse environments. The presence of 270 L. monocytogenes and 149 L. innocua strain-specific genes (clustered in 100 and 63 islets, respectively) suggests that virulence in Listeria results from multiple gene acquisition and deletion events.
Subject(s)
Bacterial Proteins/genetics , Genome, Bacterial , Listeria monocytogenes/genetics , Listeria/genetics , Adaptation, Physiological , Amino Acid Motifs , Bacillus subtilis/genetics , Bacterial Proteins/chemistry , Bacterial Proteins/physiology , Base Composition , Carrier Proteins/chemistry , Carrier Proteins/genetics , Chromosomes, Bacterial/genetics , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Gene Transfer, Horizontal , Genes, Bacterial , Genomics , Listeria/chemistry , Listeria/physiology , Listeria monocytogenes/chemistry , Listeria monocytogenes/pathogenicity , Listeria monocytogenes/physiology , Membrane Proteins/chemistry , Membrane Proteins/genetics , Sequence Analysis, DNA , Staphylococcus aureus/genetics , Transcription Factors/chemistry , Transcription Factors/genetics , Virulence/geneticsABSTRACT
We have sequenced a region containing 32.5 kb of the right arm of chromosome IV of Saccharomyces cerevisiae. Twenty open reading frames (ORFs) greater than 100 amino acids could be identified in this region. Six ORFs correspond to known yeast genes, including DOA4, UBC5 and UBC3, the gene products of which are involved in ubiquitin metabolism. UBC5 is preceded by the two tRNA genes tRNA-Arg2 and tRNA-Asp. Six genes were discovered with homologies to non-yeast genes or with homologies to other yeast ORFs. One of these could be identified as ribosomal protein gene RPS13. The putative function of eight ORFs remains unclear because comparison to different DNA or protein databases revealed no significant patterns. The sequence from cosmid 2F21 was obtained entirely by a combined subcloning and walking primer strategy, and has been deposited in the EMBL data library under Accession Number X84162.
