ABSTRACT
Peroxisomes import their luminal proteins from the cytosol. Most substrates contain a C-terminal Ser-Lys-Leu (SKL) sequence that is recognized by the receptor Pex5. Pex5 binds to peroxisomes via a docking complex containing Pex14, and recycles back into the cytosol following its mono-ubiquitination at a conserved Cys residue. The mechanism of peroxisome protein import remains incompletely understood. Here, we developed an in vitro import system based on Xenopus egg extracts. Import is dependent on the SKL motif in the substrate and on the presence of Pex5 and Pex14, and is sustained by ATP hydrolysis. A protein lacking an SKL sequence can be coimported, providing strong evidence for import of a folded protein. The conserved cysteine in Pex5 is not essential for import or to clear import sites for subsequent rounds of translocation. This new in vitro assay will be useful for further dissecting the mechanism of peroxisome protein import.
Subject(s)
Cell Extracts/analysis , Oocytes/metabolism , Peroxisome-Targeting Signal 1 Receptor/metabolism , Peroxisomes/metabolism , Xenopus Proteins/metabolism , Xenopus laevis/metabolism , Animals , Cytosol/metabolism , Female , Oocytes/cytology , Peroxisome-Targeting Signal 1 Receptor/genetics , Protein Transport , Ubiquitination , Xenopus Proteins/genetics , Xenopus laevis/genetics , Xenopus laevis/growth & developmentABSTRACT
The Pex1 and Pex6 proteins are members of the AAA family of ATPases and are involved in peroxisome biogenesis. Recently, cryo-electron microscopy structures of the Pex1-Pex6 complex in different nucleotide states have been determined. This Structural Snapshot describes the structural features of the complex and their implications for its function, as well as questions that still await answers.
Subject(s)
Adenosine Triphosphatases/chemistry , Adenosine Triphosphatases/metabolism , Membrane Proteins/chemistry , Membrane Proteins/metabolism , Peroxisomes/enzymology , ATPases Associated with Diverse Cellular Activities , Cryoelectron Microscopy , Humans , Models, Molecular , Multienzyme Complexes/chemistry , Multienzyme Complexes/metabolism , Protein Conformation , Protein Interaction Domains and Motifs , Protein Subunits , Static ElectricityABSTRACT
Members of the AAA family of ATPases assemble into hexameric double rings and perform vital functions, yet their molecular mechanisms remain poorly understood. Here, we report structures of the Pex1/Pex6 complex; mutations in these proteins frequently cause peroxisomal diseases. The structures were determined in the presence of different nucleotides by cryo-electron microscopy. Models were generated using a computational approach that combines Monte Carlo placement of structurally homologous domains into density maps with energy minimization and refinement protocols. Pex1 and Pex6 alternate in an unprecedented hexameric double ring. Each protein has two N-terminal domains, N1 and N2, structurally related to the single N domains in p97 and N-ethylmaleimide sensitive factor (NSF); N1 of Pex1 is mobile, but the others are packed against the double ring. The N-terminal ATPase domains are inactive, forming a symmetric D1 ring, whereas the C-terminal domains are active, likely in different nucleotide states, and form an asymmetric D2 ring. These results suggest how subunit activity is coordinated and indicate striking similarities between Pex1/Pex6 and p97, supporting the hypothesis that the Pex1/Pex6 complex has a role in peroxisomal protein import analogous to p97 in ER-associated protein degradation.
Subject(s)
Adenosine Triphosphatases/chemistry , Membrane Proteins/chemistry , Saccharomyces cerevisiae Proteins/chemistry , Saccharomyces cerevisiae/chemistry , ATPases Associated with Diverse Cellular Activities , Adenosine Diphosphate/chemistry , Adenosine Triphosphate/analogs & derivatives , Adenosine Triphosphate/chemistry , Chromatography, Gel , Computer Simulation , Cryoelectron Microscopy , Endoplasmic Reticulum/chemistry , Hydrolysis , Monte Carlo Method , N-Ethylmaleimide-Sensitive Proteins/chemistry , Peptides/chemistry , Peroxisomes/chemistry , Protein Structure, TertiaryABSTRACT
Organophosphorus (OP) based pesticides are known powerful inhibitors of cholinesterases, thus the toxicity of this class of compounds causes serious environmental and human health concerns. We report that benzodipyrido[3,2-a:2',3'-c]phenazine (BDPPZ) and 3,6-dimethylbenzodipyrido-[3,2-a:2',3'-c]phenazine (DM-BDPPZ) provide independent fluorescent and electrochemical signal transductions in the presence of the organophosphorus (OP) pesticides; fenthion, malathion and ethion. The presence of the methyl groups at the 3 and 6 positions in DM-BDPPZ was found to significantly influence the sensor performance. The difference in the fluorescence and electrochemical signals produced by the interaction of the sensor compound with each of the OP pesticides provides a means for differentiating between the three pesticides. Detection limits of 10(-8)M, 10(-9) and 10(-12)M were obtained for fenthion, malathion and ethion, respectively. Due to the high sensitivity and ability to minimize false positives these new sensors will be useful for potential integration for future environmental use.
