[Effect of gene db dose on the development of beta-cytotropic and immunotropic effects of long-term glibenclamide administration in C57BL/KsJY mice]. / Vliianie dozy gena db na razvitie beta-tsitotropnogo i immunotropnogo éffektov dlitel'nogo vvedeniia glibenklamida u myshei C57BL/KsJY.

The studies have revealed a modifying influence of gene db on the expressivity of damaging beta-cytotropic effect of long-term sulphanilamide therapy and on the development of autoimmune cell response to antigens of pancreatic islets. It is substantiated as promising to study a significance of individual genes and their doses in determining the variations of reactivity to beta-cytotropic and immunotropic effects of the chemical nature factors.

[Expression of the db (diabetes) gene in mice with hereditary load by generalized autoimmune pathology]. / Ekspressiia gena db (diabetes) u myshei s nasledstvennoi otiagoshchennost'iu po generalizovannoi autoimmunnoi patologii.

A model of genetically determinate diabetes mellitus in hybrid db/db mice with hereditary load by generalized autoimmune pathology has been described. The data on the character of hormonal-metabolic disturbances permit a conclusion on more serious course of diabetes mellitus in mice (C57Bl/Ks x NZB)F2 db/db as against (C57BL/Ks x NZW)F2 db/db, that is correlated with expression of autoimmune pathology in parent lines of New Zealand mice NZB and NZW. It is stated that diabetic syndrome in males proceeds in more serious form than in females.

[The effect of glibenclamide on the spontaneous evolution of genetically determined diabetes mellitus in C57BL/KsJY db+/+db mice]. / Vliianie glibenklamida na spontannuiu évoliutsiiu geneticheski determinirovannogo sakharnogo diabeta u myshei linii C57BL/KsJY db+/+db.

The study was carried out on 25 mice of the mutant C57BL/KsJY line carrying the autosomal-recessive gene db (diabetes) in the homozygous state with basal normo- and hyperglycemia by the beginning of the treatment with glybenclamide (the latent and manifest stages of insulin-independent diabetes mellitus). It was found that long-term oral administration of the drug in the therapeutic dose (20 micrograms per mice a day for 3-3.5 months) enhanced the genetically determined disturbances of glucose homeostasis and the insulin-producing apparatus of the pancreas irrespective of the stage of spontaneous diabetes genesis. The development of the organ-specific autoimmune reactions directed to antigens of the pancreatic islands was found.

[Effect of mutant genes db(diabetes) and m(misty) on the severity of high-dose streptozotocin diabetes in C57bl/KsJY mice]. / Vliianie mutantnykh genov db(diabetes) i m(misty) na tiazhest' vysokodozovogo streptozototsinovogo diabeta u myshei linii C57bl/KsJY.

It is stated that high-dose streptozotocin diabetes is more serious in gene db-homozygous mice of mutant line C57BL/KsJY than in heterozygotes db +/+ m and mice M+/+m. Manifestation of spontaneous insulin-independent diabetes mellitus in homozygotes db decreases the level of hyperglycemic streptozotocin-induced reactions.

[Study of diabetogenic and lympholytic effects during single and fractional administration of streptozotocin to mice of the mutant strain C57BL/KsIY-db/m]. / Issledovanie diabetogennykh i limfoliticheskikh éffektov pri odnokratnom i drobnom vvedenii streptozototsina mysham mutantnoi linii C57BL/KsIY-db/m.

Single administration of streptozotocin at a dose of 200 mg/kg into mice males of the C57BL/KsJY db/m strain induced severe form of insulin-dependent diabetes within the earlier periods as compared with fractional injection of the drug (40 mg/kg daily within 5 days). Streptozotocin-induced diabetes, developed after single administration of the drug, caused pronounced destructions in lymphoid tissue, primarily in thymus as content of DNA was drastically decreased in the tissue and level of DNA breakdown products was increased. The lympholytic effect of streptozotocin was less distinct after fractional administration.

[The effect of irradiation on the development of streptozotocin diabetes in C57BL/KsJY mice heterozygous and homozygous for gene db (diabetes)]. / Vliianie oblucheniia na razvitie streptozototsinovogo diabeta u geterozigotnykh i gomozigotnykh po genu db (diabetes) myshei linii C57BL/KsJY.

In experiments with C57BL/KsJY mice carrying a mutant db gene at the heterozygous and homozygous states it has been found that db+/+db mice are much more sensitive to gamma radiation with respect to the survival rate. In homozygotes, in contrast to heterozygotes, irradiation with a dose of 6 Gy does not reduce the severity of the diabetogenic effect of low doses of streptozotocin.

[The effect of long-term glibenclamide administration on pancreatic beta cells and on autoimmunity indices]. / Vliianie dlitel'nogo vvedeniia glibenklamida na pankreaticheskie beta-kletki i nekotorye pokazateli autoimmuniteta.

It was found that administration of glybenclamide in the therapeutic dose to rats exerts the damaging effect on insulin-producing apparatus of the pancreas (inhibition of the secretory reaction of beta-cells, disturbance of their morphological structure, decrease of glucose tolerance test), initiates the development of lymphocellular infiltration of islands (autoimmune insulitis), increases the level of antibodies to blood serum DNA. The inverse temporal dependence between the appearance and degree of diabetogenic effects of chronic sulfanylamide therapy and changes in autoimmunity (the latters are characteristic of earlier treatment with the drug) can be traced.

[Importance of db gene for the development of low dose streptozotocin diabetes in C57BL/KsJ mice]. / Znachenie gena db dlia razvitiia nizkodozovogo streptozototsinovogo diabeta u myshei linii C57BL/KsJ.

The paper is concerned with some data on the effect of the diabetic gene (db) on mouse sensitivity to streptozotocin (SC). Male mice aged 2-3 mos. of mutant C57BL/KsJ strain (genotypes: m+/+m, db+/+m, db+/+db) were used for investigation. Insulin-dependent diabetes mellitus was induced by intraperitoneal injections of streptozotocin at a daily dose of 40 mg/kg for 5 days. The structure and function of the insular apparatus were histologically assessed as well as by the blood level of insulin and glucose within 15 days after the start of the experiment. The earliest hyperglycemic reaction to SC was typical of mice, homozygous by the diabetic gene; they had normoglycemia at the time of treatment. In mice, heterozygous by the diabetic gene, a hyperglycemic reaction developed later after treatment. However by the end of the investigation it reached values which were typical of mice, homozygous by the diabetic gene, with basal normoglycemia. Mice, not carrying the diabetic gene, as well as homozygotes by this gene with basal hyperglycemia, possessed lesser sensitivity to SC. The expression of hyperglycemic reactions showed correlation with a degree of dystrophic changes and the development of lymphocellular infiltration in the pancreatic islets of mice with basal normoglycemia in low dose streptozotocin diabetes. The development of spontaneous hyperglycemia in homozygotes by the diabetic gene lowered their sensitivity to SC diabetogenic effects.

[Microbiological determination of DNA levels in blood leukocytes]. / Mikrobiologicheskoe opredelenie kontsentratsii DNK v leikotsitakh krovi.

A procedure is developed for estimation of DNA microquantities in biological samples, which involved acid hydrolysis of DNA and the following microbiological estimation of its concentration. A sensitivity of the procedure was as little as 0.25 micrograms DNA per ml. At the DNA concentration 0.25-10 micrograms/ml growth of the indicator strain of bacteria exhibited the linear shape. As shown by means of the procedure developed one rat leukocyte contained 6.3 +/- 0.6 X 10(-12) g of DNA.

[Possibility of biological indication of radiation injury from the blood levels of deoxynucleosides and deoxynucleotides in combined radiation-mechanical injuries]. / Vozmozhnost' biologicheskoi indikatsii luchevogo porazheniia po soderzhaniiu dezoksinukleozidov i dezoksinukleotidov v krovi pri radiatsionno-mekhanicheskom porazhenii.

A study was made of the dependence of a total content of deoxynucleosides and deoxynucleotides in blood serum and blood clot leukocytes upon radiation dose and time lapsed after radiation- and radiation-mechanical affection. It was established that after radiation-mechanical affection this dependence was different from that observed after the effect of radiation alone. From the analysis of the data obtained it was inferred that deoxynucleosides and deoxynucleotides could be used for biological indication of radiation damage in conditions of radiation-mechanical affection.

[RNA and nuclear protein synthesis in regenerating and uninjured rabbit tissues]. / Sintez RNK i iadernykh belkov v regeneriruiushchei i netravmirovannykh tkaniakh krolikov.

Synthesis of RNA and nuclear proteins was activated in regenerating rabbit muscles within 2-8 days after trauma; within the subsequent periods (13-31 days) it was comparable with control values in most cases. The trauma affected the RNA synthesis in several other nontraumatized tissues of animals.